P96041 · SYA_SACS2

Function

function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

1900100200300400500600700800900
TypeIDPosition(s)Description
Binding site604Zn2+ (UniProtKB | ChEBI)
Binding site608Zn2+ (UniProtKB | ChEBI)
Binding site708Zn2+ (UniProtKB | ChEBI)
Binding site712Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionalanine-tRNA ligase activity
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processalanyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alanine--tRNA ligase
  • EC number
  • Alternative names
    • Alanyl-tRNA synthetase
      (AlaRS
      )

Gene names

    • Name
      alaS
    • Ordered locus names
      SSO0341

Organism names

Accessions

  • Primary accession
    P96041

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000752771-900Alanine--tRNA ligase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    900
  • Mass (Da)
    102,980
  • Last updated
    2001-06-01 v2
  • Checksum
    6CB15A31210E153F
MKASEEEYKLNFFIKNDFKRKICKSCQTPFWTKDDKKEYCSDIPCTDYYFFDINIKSQPLTVKEAREKFLSFFEKRGHTRIPPKPVLARWREDLYLTIASIVDFQPHVTSGLVPPPANPLVVSQPSIRLEDIDNVGVTFGRHLTTFEMAAHHAFNYPDRYVYWKDETTAYATEFFTKELGIPEEELNFKESWWEGGGNAGPCLEVTVGGLELATLVFMQYKITDNGDYIPLKLKIVDTGYGVERIAWVTQKTPSAFHAIYGNLVYKFFDKIGVAYIDETLLKVASRFAGKIDPDNPDTIKIHRQMVSKELGIDIKNVEEELDRAAKVFQILDHTKTIMLMLADGLVPSNSGEGYLGRLVIRRALKVLRLLKSDVRLYELVKEQIGFWKEDFPQVLKNKDYILDAVELEQQRFEKILEKVPSIASTLARKSEITTEDLIQVYDSNGVPPDLLEEELKKKRVKFELPRNFYALVAKRHQTSTIKNVYDKVKLPKDLLEYITTLQPTEKLYYKDQYMRSFEGKVLGIYKNYLILDKTTFYPEGGGQLGDTGLIIDEKSSKRYEVVDTQKVNDVIIHVLKEEPSTIKVGDNVRGEINWERRYRLMRHHTVTHVILAAAKKVLGDHIWQAGAEKTPEKGRLDITHHKALTEEEVKLIENYANSVISDRRPVKPLEMNRMEAEMKYGVSIYEGGVPNSATIRLLEIKDWDIESCGGTHVSNTSEIGAVKIINVERIQDGIIRLEYVAGPALVDYIRETEAKIAEASKIIGTSPDQLPSRLRRILNEVEKKNNLIIQYRRIVETELLNSLKPYEINGNKIYIIEGLNDEEENKEILRKLTSTDNTIAISISDNRLQIATSKNMRVDKIVEELLKGGGKGGGKGTFANVILSSKKSKEEIIDIVRKSL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE006641
EMBL· GenBank· DDBJ
AAK40671.1
EMBL· GenBank· DDBJ
Genomic DNA
U85262
EMBL· GenBank· DDBJ
AAB99528.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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