P95867 · TREZ_SACS2

Function

Catalytic activity

  • hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-[(1->4)-alpha-D-glucanosyl]n trehalose to yield trehalose and (1->4)-alpha-D-glucan.
    EC:3.2.1.141 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
7.22 mMmaltotriosyltrehalose
5.66 mMmaltotetraosyltrehalose
5.89 mMmaltopentaosyltrehalose
5.86 mMmaltopentaose
5.63 mMmaltohexaose
2.63 mMmaltoheptaose

pH Dependence

Optimum pH is 5.0.

Temperature Dependence

Optimum temperature is 85 degrees Celsius.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site253-258substrate
Active site255Nucleophile
Active site286Proton donor
Binding site311-315substrate
Binding site379-384substrate
Site380Transition state stabilizer

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity
Biological Processtrehalose biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • CBM48Carbohydrate-Binding Module Family 48
    • GH13Glycoside Hydrolase Family 13

Names & Taxonomy

Protein names

  • Recommended name
    Malto-oligosyltrehalose trehalohydrolase
  • EC number
  • Short names
    MTHase
  • Alternative names
    • 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
    • Maltooligosyl trehalose trehalohydrolase

Gene names

    • Name
      treZ
    • Ordered locus names
      SSO2093

Organism names

Accessions

  • Primary accession
    P95867

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis21815-fold and 150-fold reduction in specific activity, respectively.
Mutagenesis255660-fold reduction in specific activity.
Mutagenesis259Insignificant change in specific activity.
Mutagenesis2861100-fold reduction in specific activity.
Mutagenesis3282-fold reduction in specific activity.
Mutagenesis355Very small decrease in specific activity.
Mutagenesis3562-fold reduction in specific activity.
Mutagenesis3808140-fold reduction in specific activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003937521-561Malto-oligosyltrehalose trehalohydrolase

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    561
  • Mass (Da)
    64,371
  • Last updated
    1997-05-01 v1
  • Checksum
    B00E403020F6B242
MTFGYKLDEDGVTFNLWAPYQRKVKLKILNRGIYEMERDDKGYFTITLDNVRVGDRYKYILDDNSEVPDPASRYQPEGVHGYSEIISPDFEWDDENSVKVKREDLVIYELHIGTFTSEGTFEGVIKKLNYLKELGVTAIEIMPIAQFPGKKDWGYDGVYLYAVQNSYGGPSGFRKLVNEAHKLGLAVILDVVYNHVGPEGNYMVKLGPYFSEKYKTPWGLTFNFDDAGSDEVRKFILENVEYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYDKIVIAESDLNDPRVVNPKEKCGYNIDAQWVDDFHHAIHAFLTGERQGYYSDFGSIGDIVKSYKDVFIYDGKYSNFRRKTHGKSVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESYKIAAALYILSPYIPMIFMGEEYGEENPFYYFSDFSDPKLIQGVREGRRRENGQETDPQSDCTFNDSKLSWKINDDILSFYKSLIKIRKEYGLACNRKLSVENGNYWLTVKGNGCLAVYVFSKSVIEMKYSGTLVLSSNSSFPSQITESKYELDKGFALYKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y08256
EMBL· GenBank· DDBJ
CAA69503.1
EMBL· GenBank· DDBJ
Genomic DNA
AE006641
EMBL· GenBank· DDBJ
AAK42272.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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