P93460 · RNS5_PYRPY
- ProteinRibonuclease S-5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids227 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Self-incompatibility (SI) is the inherited ability of a flowering plant to prevent self-fertilization by discriminating between self and non-self pollen during pollination. In many species, self-incompatibility is controlled by the single, multiallelic locus S.
Catalytic activity
- a ribonucleotidyl-ribonucleotide-RNA + H2O = a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 36 | a 3'-terminal ribonucleotide 3'-phosphate residue (UniProtKB | ChEBI) of RNA (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 60 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 60 | a 3'-terminal ribonucleotide 3'-phosphate residue (UniProtKB | ChEBI) of RNA (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 97-98 | a 3'-terminal ribonucleotide 3'-phosphate residue (UniProtKB | ChEBI) of RNA (UniProtKB | ChEBI) | ||||
Sequence: NV | ||||||
Binding site | 107 | a 3'-terminal ribonucleotide 3'-phosphate residue (UniProtKB | ChEBI) of RNA (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 110-111 | a 3'-terminal ribonucleotide 3'-phosphate residue (UniProtKB | ChEBI) of RNA (UniProtKB | ChEBI) | ||||
Sequence: KE | ||||||
Active site | 111 | |||||
Sequence: E | ||||||
Binding site | 114-115 | a 3'-terminal ribonucleotide 3'-phosphate residue (UniProtKB | ChEBI) of RNA (UniProtKB | ChEBI) | ||||
Sequence: KH | ||||||
Active site | 115 | Proton acceptor | ||||
Sequence: H |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | ribonuclease T2 activity | |
Molecular Function | RNA binding | |
Biological Process | RNA catabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease S-5
- EC number
- Alternative names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Rosales > Rosaceae > Amygdaloideae > Maleae > Pyrus
Accessions
- Primary accessionP93460
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-27 | |||||
Sequence: MGITGMVYVVTMVFLLIVLILSSSTVG | ||||||
Chain | PRO_0000030981 | 28-227 | Ribonuclease S-5 | |||
Sequence: YDYFQFTQQYQLAVCNSNRTPCKDPPDKLFTVHGLWPSSMAGPDPSNCPIRNIRKREKLLEPQLAIIWPNVFDRTKNKLFWDKEWMKHGTCGYPTIDNENHYFETVIKMYISKKQNVSRILSKAKIEPDGKKRALLDIENAIRNGADNKKPKLKCQKKGTTTELVEITLCSDKSGEHFIDCPHPFEPISPHYCPTNNIKY | ||||||
Disulfide bond | 42↔49 | |||||
Sequence: CNSNRTPC | ||||||
Glycosylation | 45 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 75↔118 | |||||
Sequence: CPIRNIRKREKLLEPQLAIIWPNVFDRTKNKLFWDKEWMKHGTC | ||||||
Glycosylation | 143 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 182↔220 | |||||
Sequence: CQKKGTTTELVEITLCSDKSGEHFIDCPHPFEPISPHYC | ||||||
Disulfide bond | 197↔208 | |||||
Sequence: CSDKSGEHFIDC |
Post-translational modification
N-glycan at Asn-45 consists of disaccharide (GlcNAc-GlcNAc). N-linked core structure at Asn-143 contains xylose.
Keywords
- PTM
PTM databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length227
- Mass (Da)26,059
- Last updated1997-05-01 v1
- Checksum44BC2A9E9E738FCA
Keywords
- Technical term