P93313 · NU4M_ARATH
- ProteinNADH-ubiquinone oxidoreductase chain 4
- GeneND4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids495 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Miscellaneous
A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of the duplicated gene (At2g07717) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.
Catalytic activity
- a ubiquinone + 5 H+(in) + NADH = a ubiquinol + 4 H+(out) + NAD+
a ubiquinone RHEA-COMP:9565 + 5 H+ (in)CHEBI:15378+ CHEBI:57945 = a ubiquinol RHEA-COMP:9566 + 4 H+ (out)CHEBI:15378+ CHEBI:57540
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial respiratory chain complex I | |
Cellular Component | plastid | |
Molecular Function | NADH dehydrogenase (ubiquinone) activity | |
Molecular Function | ubiquinone binding | |
Biological Process | aerobic respiration | |
Biological Process | ATP synthesis coupled electron transport | |
Biological Process | electron transport coupled proton transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-ubiquinone oxidoreductase chain 4
- EC number
- Alternative names
Gene names
Encoded on
- Mitochondrion
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP93313
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 9-29 | Helical | ||||
Sequence: YFNLSGLILCPVLGSIILLFI | ||||||
Transmembrane | 39-59 | Helical | ||||
Sequence: LIGLCASLITFLYSLVLWIQF | ||||||
Transmembrane | 89-109 | Helical | ||||
Sequence: ISLFFVILTTFLIPICILVGW | ||||||
Transmembrane | 118-138 | Helical | ||||
Sequence: EYIIAFLICEFLMIAVFCMLD | ||||||
Transmembrane | 173-193 | Helical | ||||
Sequence: FFLYTLLGSLFMLLAILLILF | ||||||
Transmembrane | 214-234 | Helical | ||||
Sequence: IFLWIAFFASFAVKVPMVPVH | ||||||
Transmembrane | 245-265 | Helical | ||||
Sequence: PTAGSVILAGILLKFGTYGFL | ||||||
Transmembrane | 272-292 | Helical | ||||
Sequence: FPEATLCFTPFIYTLSAIAII | ||||||
Transmembrane | 335-355 | Helical | ||||
Sequence: ILLMLSHGLVSSALFLCVGVL | ||||||
Transmembrane | 367-387 | Helical | ||||
Sequence: YGGLVSTMPNFSTIFFFFTLA | ||||||
Transmembrane | 413-433 | Helical | ||||
Sequence: LVATLAALGMILGAAYSLWLY | ||||||
Transmembrane | 457-477 | Helical | ||||
Sequence: VFIFIPFLVGVVWMGVYPKVF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000117889 | 1-495 | NADH-ubiquinone oxidoreductase chain 4 | |||
Sequence: MLEHFCECYFNLSGLILCPVLGSIILLFIPNSRIRLIRLIGLCASLITFLYSLVLWIQFDSSTAKFQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYIIAFLICEFLMIAVFCMLDLLLFYVFFESVLIPMFIIIGVWGSRQRKIKAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQILLTTEFSERRQIFLWIAFFASFAVKVPMVPVHIWLPEAHVEAPTAGSVILAGILLKFGTYGFLRFSIPMFPEATLCFTPFIYTLSAIAIIYTSLTTLRQIDLKKIIAYSSVAHMNLVTIGMFSLNIQGIGGSILLMLSHGLVSSALFLCVGVLYDRHKTRLVRYYGGLVSTMPNFSTIFFFFTLANMSLPGTSSFIGEFLILVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDLNGREVFIFIPFLVGVVWMGVYPKVFLDCMHTSVSNLVQHGKFH |
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Sequence similarities
Belongs to the complex I subunit 4 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length495
- Mass (Da)55,942
- Last updated2023-09-13 v3
- Checksum7B70B7EB8BF5EC6B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 146 | in Ref. 1; CAA69742 | ||||
Sequence: F → P | ||||||
Sequence conflict | 326 | in Ref. 1; CAA69742 | ||||
Sequence: L → P | ||||||
Sequence conflict | 383 | in Ref. 1; CAA69742 | ||||
Sequence: F → S | ||||||
Sequence conflict | 467 | in Ref. 1; CAA69742 | ||||
Sequence: V → L |
RNA Editing
Edited at positions 10 25 36 53 55 56 66 106 121 126 135 150 203 220 256 262 279 299 337 345 377 391 452 458 469 473 478
Keywords
- Coding sequence diversity
- Technical term