P93111 · HEM11_CUCSA
- ProteinGlutamyl-tRNA reductase 1, chloroplastic
- GeneHEMA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids552 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).
Miscellaneous
During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).
Catalytic activity
- (S)-4-amino-5-oxopentanoate + NADP+ + tRNA(Glu) = H+ + L-glutamyl-tRNA(Glu) + NADPH
Pathway
Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 150-153 | substrate | ||||
Sequence: TCNR | ||||||
Active site | 151 | Nucleophile | ||||
Sequence: C | ||||||
Site | 200 | Important for activity | ||||
Sequence: H | ||||||
Binding site | 210 | substrate | ||||
Sequence: S | ||||||
Binding site | 215-217 | substrate | ||||
Sequence: EGQ | ||||||
Binding site | 221 | substrate | ||||
Sequence: Q | ||||||
Binding site | 292-297 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GAGKMG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | glutamyl-tRNA reductase activity | |
Molecular Function | NADP binding | |
Biological Process | chlorophyll biosynthetic process | |
Biological Process | protoporphyrinogen IX biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA reductase 1, chloroplastic
- EC number
- Short namesGluTR
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Cucurbitales > Cucurbitaceae > Benincaseae > Cucumis
Accessions
- Primary accessionP93111
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, transit peptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000013309 | ?-552 | Glutamyl-tRNA reductase 1, chloroplastic | |||
Sequence: MAVSTSFSGAKLEALLFKSASNSSSTRNLSSSHLPGFCKSIRTRRILFQRTGVSSFTPFKCELASSDVLVQNDEIDPPKSSNLSALEQLKTSAVDRYTKERSSIVVIGLSIHTTPVEMREKLAIPEAEWPRAIGELCGLNHIEEAAVLSTCNRMEIYVVALSQHRGVKEVTEWMSKTSGIPVSEICQHRFLLYNNDATQHIFEVSAGLDSLVLGEGQILAQVKQVVKVGQGVAGFGRNISGLFKHAITVGKRVRTETNIAAGAVSVSSAAVELALMKLPEPSHATARMLVIGAGKMGKLVIKHLVAKGCTKMVVVNRSEERVTAIREEMKDVEIIYKPLTEMLSCTAEADVIFTSTASESLLFTKEQVKDLPPVGHDVGGLRLFIDISVPRNVGACINNLEDVRVYNVDDLKEVVAANKEDRLRKAMEAQSIITEESKQFEAWRDSLETVPTIKKLRAYAERIRTAELEKCLSKMGDDIPKKTRRAVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALNRMFSLETEIAVLEQKIRAKVEQNQK | ||||||
Transit peptide | 1-? | Chloroplast |
Expression
Tissue specificity
Primarily in cotyledons and hypocotyls of greening cucumber seedlings.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length552
- Mass (Da)60,895
- Last updated1997-05-01 v1
- Checksum9C7A72AF24FF00F5
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D50407 EMBL· GenBank· DDBJ | BAA08910.1 EMBL· GenBank· DDBJ | mRNA |