P93111 · HEM11_CUCSA

  • Protein
    Glutamyl-tRNA reductase 1, chloroplastic
  • Gene
    HEMA1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    3/5

Function

function

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site150-153substrate
Active site151Nucleophile
Site200Important for activity
Binding site210substrate
Binding site215-217substrate
Binding site221substrate
Binding site292-297NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functionglutamyl-tRNA reductase activity
Molecular FunctionNADP binding
Biological Processchlorophyll biosynthetic process
Biological Processprotoporphyrinogen IX biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamyl-tRNA reductase 1, chloroplastic
  • EC number
  • Short names
    GluTR

Gene names

    • Name
      HEMA1

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Aonagajibai
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Cucurbitales > Cucurbitaceae > Benincaseae > Cucumis

Accessions

  • Primary accession
    P93111

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, transit peptide.

TypeIDPosition(s)Description
ChainPRO_0000013309?-552Glutamyl-tRNA reductase 1, chloroplastic
Transit peptide1-?Chloroplast

Expression

Tissue specificity

Primarily in cotyledons and hypocotyls of greening cucumber seedlings.

Structure

Family & Domains

Sequence similarities

Belongs to the glutamyl-tRNA reductase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    552
  • Mass (Da)
    60,895
  • Last updated
    1997-05-01 v1
  • Checksum
    9C7A72AF24FF00F5
MAVSTSFSGAKLEALLFKSASNSSSTRNLSSSHLPGFCKSIRTRRILFQRTGVSSFTPFKCELASSDVLVQNDEIDPPKSSNLSALEQLKTSAVDRYTKERSSIVVIGLSIHTTPVEMREKLAIPEAEWPRAIGELCGLNHIEEAAVLSTCNRMEIYVVALSQHRGVKEVTEWMSKTSGIPVSEICQHRFLLYNNDATQHIFEVSAGLDSLVLGEGQILAQVKQVVKVGQGVAGFGRNISGLFKHAITVGKRVRTETNIAAGAVSVSSAAVELALMKLPEPSHATARMLVIGAGKMGKLVIKHLVAKGCTKMVVVNRSEERVTAIREEMKDVEIIYKPLTEMLSCTAEADVIFTSTASESLLFTKEQVKDLPPVGHDVGGLRLFIDISVPRNVGACINNLEDVRVYNVDDLKEVVAANKEDRLRKAMEAQSIITEESKQFEAWRDSLETVPTIKKLRAYAERIRTAELEKCLSKMGDDIPKKTRRAVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALNRMFSLETEIAVLEQKIRAKVEQNQK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D50407
EMBL· GenBank· DDBJ
BAA08910.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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