P92514 · COX3_ARATH
- ProteinCytochrome c oxidase subunit 3
- GeneCOX3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids265 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Miscellaneous
A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of this duplicated gene (At2g07687) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.
Catalytic activity
- 4 Fe(II)-[cytochrome c] + 8 H+(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H+(out) + 2 H2OThis reaction proceeds in the forward direction.
4 RHEA-COMP:10350 + 8 H+ (in)CHEBI:15378+ CHEBI:15379 = 4 RHEA-COMP:14399 + 4 H+ (out)CHEBI:15378+ 2 CHEBI:15377
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Molecular Function | cytochrome-c oxidase activity | |
Biological Process | mitochondrial electron transport, cytochrome c to oxygen |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome c oxidase subunit 3
- EC number
- Alternative names
Gene names
Encoded on
- Mitochondrion
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP92514
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 41-61 | Helical | ||||
Sequence: GGARLLSLGLIFILYTMFVWW | ||||||
Transmembrane | 85-105 | Helical | ||||
Sequence: GFILFIVSEVMFFFAFFWAFF | ||||||
Transmembrane | 137-157 | Helical | ||||
Sequence: TLILLSSGAAVTWAHHAILAG | ||||||
Transmembrane | 162-182 | Helical | ||||
Sequence: AVYALVATVLLALVFTGFQGM | ||||||
Transmembrane | 200-220 | Helical | ||||
Sequence: FFLATGFHGFHVIIGTLFLII | ||||||
Transmembrane | 245-265 | Helical | ||||
Sequence: WHFVDVVWLFLFVSIYWWGGI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000183737 | 1-265 | Cytochrome c oxidase subunit 3 | |||
Sequence: MIESQRHSYHLVDPSPWPISGSLGALATTVGGVMYMHSFQGGARLLSLGLIFILYTMFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFFFAFFWAFFHSSLAPAVEIGGIWPPKGIEVLDPWEIPFLNTLILLSSGAAVTWAHHAILAGKEKRAVYALVATVLLALVFTGFQGMEYYQAPFTISDSIYGSTFFLATGFHGFHVIIGTLFLIICGIRQYLGHLTKEHHVGFEAAAWYWHFVDVVWLFLFVSIYWWGGI |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length265
- Mass (Da)29,961
- Last updated2001-05-04 v2
- Checksum753978DE1CAA13F1
RNA Editing
Edited at positions 38 82 86 104 105 138 141
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y08501 EMBL· GenBank· DDBJ | CAA69818.3 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
JF729200 EMBL· GenBank· DDBJ | AEK01262.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
JF729201 EMBL· GenBank· DDBJ | AEK01298.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
JF729202 EMBL· GenBank· DDBJ | AEK01326.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BK010421 EMBL· GenBank· DDBJ | DAB41522.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC007143 EMBL· GenBank· DDBJ | AAM15412.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002685 EMBL· GenBank· DDBJ | AEC06078.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
EF488906 EMBL· GenBank· DDBJ | ABS50618.1 EMBL· GenBank· DDBJ | mRNA | ||
EF488907 EMBL· GenBank· DDBJ | ABS50619.1 EMBL· GenBank· DDBJ | mRNA |