P91277 · HRPK1_CAEEL
- ProteinHeterogeneous nuclear ribonucleoprotein K homolog
- Genehrpk-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids397 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
RNA-binding protein which functions together with alg-1, a component of the miRNA loading complex, to modulate the processing and activity of specific miRNAs such as miR-58 and let-7 to regulate gene expression at the post-transcriptional level during embryonic, hypodermal and neuronal development (PubMed:31584932).
Promotes the lsy-6-mediated repression of cog-1 in uterine cells (PubMed:31584932).
In embryos, may play a role in the DNA damage response (PubMed:15654100).
Promotes the lsy-6-mediated repression of cog-1 in uterine cells (PubMed:31584932).
In embryos, may play a role in the DNA damage response (PubMed:15654100).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | mRNA binding | |
Biological Process | DNA damage response | |
Biological Process | embryo development ending in birth or egg hatching | |
Biological Process | regulation of mRNA splicing, via spliceosome | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | regulatory ncRNA-mediated gene silencing |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHeterogeneous nuclear ribonucleoprotein K homolog
- Short nameshnRNP K
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionP91277
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Temperature sensitive with 35% embryonic lethality at 20 degrees Celsius and 80% at 25 degrees Celsius (PubMed:31584932).
Of the surviving offspring, 30% are sterile at 20 degrees Celsius and 76% are sterile at 25 degrees Celsius (PubMed:31584932).
The majority of non-sterile animals have a reduced brood size (PubMed:31584932).
In addition, animals have gonadal formation defects and display a bursting vulva phenotype (PubMed:31584932).
Reduced number of miRNAs including miR-58 in embryos and L4 larvae (PubMed:31584932).
Defective neuronal cell fate switching, whereby fewer ASEL neurons adopt an ASER cell fate in an lsy-6 mutant background (PubMed:31584932).
In young adults, increased number of seam cells and enhanced hypodermal development defects in the double miR-48 and miR-241 mutant (PubMed:31584932).
The majority of mutants are embryonic lethal in a miR-35-42 mutant background (PubMed:31584932).
Enhanced defects in hypodermal development and alae formation in a let-7 mutant background (PubMed:31584932).
RNAi-mediated knockdown relieves the lys-6-mediated repression of cog-1 in uterine cells (PubMed:31584932).
RNAi-mediated knockdown results in increased sensitivity to the DNA-damage agent methyl methanesulfonate in embryos, but does not disrupt the morphology of the nucleus (PubMed:15654100).
RNAi-mediated knockdown enhances the bursting vulva phenotype in a let-7 mutant background (PubMed:31584932).
Of the surviving offspring, 30% are sterile at 20 degrees Celsius and 76% are sterile at 25 degrees Celsius (PubMed:31584932).
The majority of non-sterile animals have a reduced brood size (PubMed:31584932).
In addition, animals have gonadal formation defects and display a bursting vulva phenotype (PubMed:31584932).
Reduced number of miRNAs including miR-58 in embryos and L4 larvae (PubMed:31584932).
Defective neuronal cell fate switching, whereby fewer ASEL neurons adopt an ASER cell fate in an lsy-6 mutant background (PubMed:31584932).
In young adults, increased number of seam cells and enhanced hypodermal development defects in the double miR-48 and miR-241 mutant (PubMed:31584932).
The majority of mutants are embryonic lethal in a miR-35-42 mutant background (PubMed:31584932).
Enhanced defects in hypodermal development and alae formation in a let-7 mutant background (PubMed:31584932).
RNAi-mediated knockdown relieves the lys-6-mediated repression of cog-1 in uterine cells (PubMed:31584932).
RNAi-mediated knockdown results in increased sensitivity to the DNA-damage agent methyl methanesulfonate in embryos, but does not disrupt the morphology of the nucleus (PubMed:15654100).
RNAi-mediated knockdown enhances the bursting vulva phenotype in a let-7 mutant background (PubMed:31584932).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000448779 | 1-397 | Heterogeneous nuclear ribonucleoprotein K homolog | |||
Sequence: MMIKVGAAINGTDSPKAMKREHDNDDGDRTGRHKRPKTDGFTEAIQQGKFEVRLLVSSKSAGAIIGKGGENIKRLRAEFNAHVQVPDSNTPERVCTVTADEKTVLNILKDVLPRLEDNFSERDPCEVRMLVHQSHAGALIGRNGSKIKELREKCSARLKIFTGCAPGSTDRVLITSGEQKNVLGIIEEVMKELKEIPIKGSATPYLPAFNYDPSNISDYGGFPGNMPAGGPPNNRGPAPQRGGQGPPGGPRSYGGAITQGGGQRSFEAGDFQQFRGGPGPVPGYAMSAPGYPPQQGQFGAPNNAGYGYGPGGGGPVTTAQVTIPSDLGGTIIGRGGERIARIRQESGAQITLEQSNGQPERIITIKGTEQQIHSAQYLLQQCVRNSTQGRERFGGSV |
Proteomic databases
Expression
Tissue specificity
Expressed in gut, muscle, neuronal and hypodermal tissues (PubMed:31584932).
Highly expressed in the germline and oocytes (PubMed:31584932).
Highly expressed in the germline and oocytes (PubMed:31584932).
Developmental stage
Ubiquitously expressed throughout development (PubMed:31584932).
Highly expressed in early embryos (PubMed:31584932).
Highly expressed in early embryos (PubMed:31584932).
Gene expression databases
Interaction
Subunit
Interacts with alg-1; the interaction is direct and may be strengthened through RNA-protein association.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P91277 | nova-1 Q9GRY9 | 3 | EBI-320476, EBI-320508 | |
BINARY | P91277 | sup-46 Q9XVS2 | 3 | EBI-320476, EBI-311961 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-41 | Disordered | ||||
Sequence: MMIKVGAAINGTDSPKAMKREHDNDDGDRTGRHKRPKTDGF | ||||||
Compositional bias | 15-40 | Basic and acidic residues | ||||
Sequence: PKAMKREHDNDDGDRTGRHKRPKTDG | ||||||
Domain | 49-111 | KH 1 | ||||
Sequence: KFEVRLLVSSKSAGAIIGKGGENIKRLRAEFNAHVQVPDSNTPERVCTVTADEKTVLNILKDV | ||||||
Domain | 124-189 | KH 2 | ||||
Sequence: PCEVRMLVHQSHAGALIGRNGSKIKELREKCSARLKIFTGCAPGSTDRVLITSGEQKNVLGIIEEV | ||||||
Region | 220-279 | Disordered | ||||
Sequence: GGFPGNMPAGGPPNNRGPAPQRGGQGPPGGPRSYGGAITQGGGQRSFEAGDFQQFRGGPG | ||||||
Domain | 316-379 | KH 3 | ||||
Sequence: VTTAQVTIPSDLGGTIIGRGGERIARIRQESGAQITLEQSNGQPERIITIKGTEQQIHSAQYLL |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 5 isoforms produced by Alternative splicing.
P91277-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length397
- Mass (Da)42,211
- Last updated1997-05-01 v1
- ChecksumDC00A065F2753E70
P91277-2
- Nameb
P91277-3
- Namec
- Differences from canonical
- 1-17: Missing
P91277-4
- Named
P91277-5
- Namee
- Differences from canonical
- 94-397: Missing
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_060454 | 1-17 | in isoform b, isoform c and isoform d | |||
Sequence: Missing | ||||||
Compositional bias | 15-40 | Basic and acidic residues | ||||
Sequence: PKAMKREHDNDDGDRTGRHKRPKTDG | ||||||
Alternative sequence | VSP_060456 | 79-84 | in isoform b | |||
Sequence: FNAHVQ → RLHRYC | ||||||
Alternative sequence | VSP_060455 | 79-88 | in isoform d | |||
Sequence: FNAHVQVPDS → VSFTGILLCF | ||||||
Alternative sequence | VSP_060457 | 85-397 | in isoform b | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_060458 | 89-397 | in isoform d | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_060459 | 94-397 | in isoform e | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284601 EMBL· GenBank· DDBJ | CCD64191.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD64192.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD64193.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD64194.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD64195.1 EMBL· GenBank· DDBJ | Genomic DNA |