P90994 · DJ11_CAEEL
- ProteinGlutathione-independent glyoxalase DJR-1.1
- Genedjr-1.1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids187 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of methylglyoxal (MG) or glyoxal (GO) to D-lactate or glycolic acid respectively in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against glyoxal-induced cell death.
Catalytic activity
- H2O + methylglyoxal = (R)-lactate + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.3 mM | methylglyoxal | |||||
3.61 mM | glyoxal |
kcat is 13.8 min-1 with methylglyoxal as substrate and 356.4 min-1 with glyoxal as substrate.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 19 | |||||
Sequence: E | ||||||
Active site | 106 | |||||
Sequence: C | ||||||
Active site | 125 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | glyoxalase III activity | |
Biological Process | cellular response to glyoxal | |
Biological Process | cellular response to methylglyoxal | |
Biological Process | glycolate biosynthetic process | |
Biological Process | glyoxal metabolic process | |
Biological Process | lactate biosynthetic process | |
Biological Process | methylglyoxal metabolic process | |
Biological Process | negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway | |
Biological Process | response to oxidative stress | |
Biological Process | response to toxic substance |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutathione-independent glyoxalase DJR-1.1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionP90994
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 106 | Completely abolishes catalytic activity. | ||||
Sequence: C → S |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000432108 | 1-187 | Glutathione-independent glyoxalase DJR-1.1 | |||
Sequence: MAQKSALIILAAEGAEEMEVIITGDVLARGEIRVVYAGLDGAEPVKCARGAHIVPDVKLEDVETEKFDIVILPGGQPGSNTLAESLLVRDVLKSQVESGGLIGAICAAPIALLSHGVKAELVTSHPSVKEKLEKGGYKYSEDRVVVSGKIITSRGPGTAFEFALKIVELLEGKDKATSLIAPMLLKL |
Proteomic databases
Expression
Structure
Sequence
- Sequence statusComplete
- Length187
- Mass (Da)19,669
- Last updated1997-05-01 v1
- ChecksumA6D124C7E8BB1AF1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FO080203 EMBL· GenBank· DDBJ | CCD61936.1 EMBL· GenBank· DDBJ | Genomic DNA |