P85298 · RHG08_HUMAN
- ProteinRho GTPase-activating protein 8
- GeneARHGAP8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids464 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Molecular Function | GTPase activator activity | |
Biological Process | negative regulation of endocytic recycling | |
Biological Process | positive regulation of ERK1 and ERK2 cascade | |
Biological Process | regulation of small GTPase mediated signal transduction | |
Biological Process | small GTPase-mediated signal transduction |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRho GTPase-activating protein 8
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP85298
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_049138 | 172 | in dbSNP:rs16992915 | |||
Sequence: Y → C | ||||||
Natural variant | VAR_049139 | 333 | in dbSNP:rs6007344 | |||
Sequence: G → R | ||||||
Natural variant | VAR_049140 | 415 | in dbSNP:rs2071762 | |||
Sequence: P → L | ||||||
Natural variant | VAR_061182 | 418 | in dbSNP:rs9614957 | |||
Sequence: P → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 869 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000308161 | 1-464 | UniProt | Rho GTPase-activating protein 8 | |||
Sequence: MAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDYTIVYFHYGLNSRNKPSLGWLQSAYKEFDRKDGDLTMWPRLVSNSKLKRSSHLSLPKYWDYRYKKNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPTKTPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELPQPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLRYLMGFLHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIFSTPEAPGEHGLAPWEQGSRAAPLQEAVPRTQATGLTKPTLPPSPLMAARRRL | |||||||
Modified residue (large scale data) | 206 | PRIDE | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 455 | PRIDE | Phosphoserine | ||||
Sequence: S |
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in kidney and placenta. Also expressed in colon, skeletal muscle, small intestine, stomach, and testis. Not detected in brain, liver or spleen. Overexpressed in the majority of colorectal tumors examined.
Gene expression databases
Organism-specific databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P85298-4 | CYGB Q8WWM9 | 3 | EBI-9523517, EBI-6309037 | |
XENO | P85298-4 | Map2k2 Q63932 | 5 | EBI-9523517, EBI-397724 | |
BINARY | P85298-4 | PIN1 Q13526 | 25 | EBI-9523517, EBI-714158 | |
BINARY | P85298-4 | SH3GL1 Q99961 | 3 | EBI-9523517, EBI-697911 | |
BINARY | P85298-4 | ZMYND12 Q9H0C1 | 3 | EBI-9523517, EBI-12030590 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 13-199 | CRAL-TRIO | ||||
Sequence: PFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDYTIVYFHYGLNSRNKPSLGWLQSAYKEFDRKDGDLTMWPRLVSNSKLKRSSHLSLPKYWDYRYKKNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEVLRYDEKLQ | ||||||
Domain | 226-412 | Rho-GAP | ||||
Sequence: VSLQYLKDKNQGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELPQPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLRYLMGFLHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIF | ||||||
Region | 439-464 | Disordered | ||||
Sequence: VPRTQATGLTKPTLPPSPLMAARRRL |
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 5 isoforms produced by Alternative splicing.
P85298-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length464
- Mass (Da)53,484
- Last updated2007-10-23 v1
- ChecksumF003A341AE5271D2
P85298-2
- Name2
P85298-3
- Name3
- Differences from canonical
- 127-131: Missing
P85298-4
- Name4
- Differences from canonical
- 101-131: Missing
P85298-5
- Name5
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_001652 | 101-131 | in isoform 2, isoform 4 and isoform 5 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_028883 | 127-131 | in isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_001656 | 325 | in isoform 2 | |||
Sequence: V → A | ||||||
Alternative sequence | VSP_054100 | 326-336 | in isoform 5 | |||
Sequence: ESSLRVTGCRQ → PGEHLQQNEQL | ||||||
Alternative sequence | VSP_001657 | 326-464 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_054101 | 337-464 | in isoform 5 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK001306 EMBL· GenBank· DDBJ | BAA91614.1 EMBL· GenBank· DDBJ | mRNA | ||
AK022305 EMBL· GenBank· DDBJ | BAB14008.1 EMBL· GenBank· DDBJ | mRNA | ||
Z83838 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
Z93244 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
Z98743 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC067824 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |