P85103 · VMXP_PHIPA

Function

function

Snake venom zinc metalloprotease that hydrolyzes the alpha chain of fibrinogen (FGA) but not the beta or gamma chains. Has caseinolytic activity. Induces hemorrhage after intradermal injection into mice. Intramuscular injection into the gastrocnemius muscle of mice causes hemorrhage, edema, myonecrosis, increase in serum creatine kinase levels and multifocal lung hemorrhage. Inhibits collagen-induced platelet aggregation, but not thrombin- or ristocetin-induced platelet aggregation.

Activity regulation

Inhibited by DTT, EDTA and L-cysteine, but not by the serine protease inhibitor PMSF. Activated by calcium, unaffected by magnesium, inhibited by zinc.

pH Dependence

Optimum pH is 7.5 with azocasein as substrate. Incubation at a pH of below 6.0 or above 9.5 results in an abrupt decrease in activity.

Temperature Dependence

Optimum temperature is 37 degrees Celsius with azocasein as substrate. Incubation at 55 degrees Celsius for 90 minutes reduces activity to 20% of its maximum.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site10Ca2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionmetal ion binding
Molecular Functionmetallopeptidase activity
Molecular Functiontoxin activity
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Snake venom metalloproteinase patagonfibrase
  • EC number
  • Short names
    SVMP

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Dipsadidae > Philodryas

Accessions

  • Primary accession
    P85103

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002847731-10Snake venom metalloproteinase patagonfibrase

Expression

Tissue specificity

Expressed by the venom gland.

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    10
  • Mass (Da)
    1,011
  • Last updated
    2010-06-15 v2
  • Checksum
    80560B6DC2C05AB1
LSTDIVAPPV

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue10

Mass Spectrometry

Molecular mass is 53,224 Da. Determined by MALDI.

Keywords

Similar Proteins

Disclaimer

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