P85103 · VMXP_PHIPA
- ProteinSnake venom metalloproteinase patagonfibrase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Snake venom zinc metalloprotease that hydrolyzes the alpha chain of fibrinogen (FGA) but not the beta or gamma chains. Has caseinolytic activity. Induces hemorrhage after intradermal injection into mice. Intramuscular injection into the gastrocnemius muscle of mice causes hemorrhage, edema, myonecrosis, increase in serum creatine kinase levels and multifocal lung hemorrhage. Inhibits collagen-induced platelet aggregation, but not thrombin- or ristocetin-induced platelet aggregation.
Activity regulation
Inhibited by DTT, EDTA and L-cysteine, but not by the serine protease inhibitor PMSF. Activated by calcium, unaffected by magnesium, inhibited by zinc.
pH Dependence
Optimum pH is 7.5 with azocasein as substrate. Incubation at a pH of below 6.0 or above 9.5 results in an abrupt decrease in activity.
Temperature Dependence
Optimum temperature is 37 degrees Celsius with azocasein as substrate. Incubation at 55 degrees Celsius for 90 minutes reduces activity to 20% of its maximum.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 10 | Ca2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | metal ion binding | |
Molecular Function | metallopeptidase activity | |
Molecular Function | toxin activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSnake venom metalloproteinase patagonfibrase
- EC number
- Short namesSVMP
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Dipsadidae > Philodryas
Accessions
- Primary accessionP85103
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000284773 | 1-10 | Snake venom metalloproteinase patagonfibrase | ||
Expression
Tissue specificity
Expressed by the venom gland.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Sequence similarities
Belongs to the venom metalloproteinase (M12B) family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length10
- Mass (Da)1,011
- Last updated2010-06-15 v2
- Checksum80560B6DC2C05AB1
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 1 | ||||
Non-terminal residue | 10 | ||||
Mass Spectrometry
Keywords
- Technical term