P85064 · SPI_SCHGR
- ProteinGreglin
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Serine protease inhibitor (PubMed:16839309, PubMed:23075397).
Inhibits porcine pancreatic elastase with a Ki of 58.3 nM, human neutrophil elastase with a Ki of 3.6 nM, cathepsin G with a Ki of 153.5 nM, chymotrypsin with a Ki of 26.7 nM and subtilisin with a Ki of 0.68 nM. Does not inhibit neutrophil protease 3 or pancreatic trypsin (PubMed:16839309).
Inhibits porcine pancreatic elastase with a Ki of 58.3 nM, human neutrophil elastase with a Ki of 3.6 nM, cathepsin G with a Ki of 153.5 nM, chymotrypsin with a Ki of 26.7 nM and subtilisin with a Ki of 0.68 nM. Does not inhibit neutrophil protease 3 or pancreatic trypsin (PubMed:16839309).
pH Dependence
No decrease in activity observed after incubating at pH 2.5, pH 7.4 and at pH 11.0 for 1 hour to overnight (PubMed:16839309, PubMed:23075397).
Temperature Dependence
Thermostable (PubMed:16839309, PubMed:23075397).
No decrease in activity was observed after heating for 1 hour at up to 95 degrees Celsius (PubMed:16839309, PubMed:23075397).
No decrease in activity was observed after heating for 1 hour at up to 95 degrees Celsius (PubMed:16839309, PubMed:23075397).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | serine-type endopeptidase inhibitor activity | |
Biological Process | negative regulation of peptidase activity |
Keywords
- Molecular function
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGreglin
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Polyneoptera > Orthoptera > Caelifera > Acrididea > Acridomorpha > Acridoidea > Acrididae > Cyrtacanthacridinae > Schistocerca
Accessions
- Primary accessionP85064
PTM/Processing
Features
Showing features for chain, modified residue, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000271408 | 1-83 | Greglin | ||
Modified residue | 8 | Phosphoserine | |||
Modified residue | 11 | Phosphoserine | |||
Modified residue | 15 | Phosphoserine | |||
Disulfide bond | 21↔55 | ||||
Disulfide bond | 25↔48 | ||||
Disulfide bond | 33↔69 | ||||
Disulfide bond | 53↔76 | ||||
Keywords
- PTM
PTM databases
Structure
Sequence
- Sequence statusComplete
- Length83
- Mass (Da)9,195
- Last updated2015-04-29 v3
- ChecksumCFEAA69ABDCC39EF
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 7 | in Ref. 1; AA sequence | |||
Sequence conflict | 17-25 | in Ref. 1; AA sequence | |||
Sequence conflict | 39 | in Ref. 1; AA sequence | |||
Mass Spectrometry
Keywords
- Technical term