P84466 · LSS_BOVIN
- ProteinLanosterol synthase
- GeneLSS
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids732 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus (PubMed:14678783).
Through the production of lanosterol may regulate lens protein aggregation and increase transparency (By similarity).
Through the production of lanosterol may regulate lens protein aggregation and increase transparency (By similarity).
Catalytic activity
- (S)-2,3-epoxysqualene = lanosterol
Activity regulation
Inhibited by the benzophenone containing OSC inhibitor Ro48-8071 and to a lesser extent by other benzophene containing inhibitors.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
11 μM | (3S)-2,3-oxidosqualine |
pH Dependence
Optimum pH is 7.4. Active from pH 5.0 to 10.0.
Temperature Dependence
Optimum temperature is 37 degrees Celsius. Active from 25 to 50 degrees Celsius.
Pathway
Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 455 | Proton donor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | lipid droplet | |
Molecular Function | lanosterol synthase activity | |
Biological Process | cholesterol biosynthetic process | |
Biological Process | steroid biosynthetic process | |
Biological Process | triterpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLanosterol synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionP84466
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylthreonine | ||||
Sequence: T | ||||||
Chain | PRO_0000072658 | 2-732 | Lanosterol synthase | |||
Sequence: TEGTCLRRRGGPYKTEPATDLSRWRLSNQVGRQTWTYSQEEDPVREQSGLEAHLLGLDTKSFFKDLPKAHTACRGALNGVTFYAALQTEDGHWAGDYGGPLFLLPGLLITCHVANIPLPAGYREEIIRYLRSVQLPDGGWGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNLLHKKGGAVFIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDWMPAHPSTIWCHCRQVYLPMAYCYSTRLSAEEGPLVQSLRQELYLEDYSCIDWAAHRNSVAPDDLYTPHSWLLHVVYAILNLYERHHSTSLRQWATQKLYEHIAADDRFTKCISIGPISKTINMLVRWHVDGPASAVFQEHVSRIPDYLWLGLDGMKMQGTNGSQIWDTAFAIQALLEARAQHRPEFWSCLRKAHEYLRISQVPDNFPDYQKYYRHMSKGGFSFSTLDCGWIVADCTAEALKSILLLQEKCPFVSNHVPRERLFDTVAVLLSLRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKTFHKQFPDHRAGEIRETLEQGLQFCRQKQRPDGSWEGSWGVCFTYGAWFGLEAFACMGHTYHNGVACAEISRACDFLLSRQMADGGWGEDFESCKQRRYVQSAQSQIHNTCWALMGLMAVRHPDVAALERGVSYLLEKQLPNGDWPQENISGVFNKSCAISYTSYRNVFPIWTLGRFSRLHPDPALAGHP |
Post-translational modification
The N-terminus is blocked.
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Detected in the liver (at protein level).
Interaction
Structure
Family & Domains
Features
Showing features for repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 124-165 | PFTB 1 | ||||
Sequence: REEIIRYLRSVQLPDGGWGLHIEDKSTVFGTALNYVSLRILG | ||||||
Repeat | 483-528 | PFTB 2 | ||||
Sequence: LFDTVAVLLSLRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDY | ||||||
Repeat | 560-600 | PFTB 3 | ||||
Sequence: LEQGLQFCRQKQRPDGSWEGSWGVCFTYGAWFGLEAFACMG | ||||||
Repeat | 612-663 | PFTB 4 | ||||
Sequence: ISRACDFLLSRQMADGGWGEDFESCKQRRYVQSAQSQIHNTCWALMGLMAVR | ||||||
Repeat | 670-712 | PFTB 5 | ||||
Sequence: LERGVSYLLEKQLPNGDWPQENISGVFNKSCAISYTSYRNVFP |
Sequence similarities
Belongs to the terpene cyclase/mutase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length732
- Mass (Da)83,184
- Last updated2006-06-27 v2
- ChecksumB06289C2FE03B4B2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ372933 EMBL· GenBank· DDBJ | ABD24094.1 EMBL· GenBank· DDBJ | mRNA |