P83953 · IMA5_RAT

  • Protein
    Importin subunit alpha-5
  • Gene
    Kpna1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed:18303947).
Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed:18303947).
Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:18303947).
At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed:18303947).
The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:18303947).
Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA2 and Transportin-1/TNPO1 (By similarity).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentdendrite
Cellular Componentglutamatergic synapse
Cellular ComponentNLS-dependent protein nuclear import complex
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentpostsynaptic density
Molecular Functionnuclear import signal receptor activity
Molecular Functionnuclear localization sequence binding
Biological ProcessNLS-bearing protein import into nucleus
Biological Processpostsynapse to nucleus signaling pathway
Biological Processregulation of apoptotic process
Biological Processregulation of canonical Wnt signaling pathway
Biological Processsatellite cell activation involved in skeletal muscle regeneration
Biological Processskeletal muscle satellite cell proliferation
Biological Processskeletal muscle tissue regeneration

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Importin subunit alpha-5
  • Alternative names
    • Importin alpha-5
    • Karyopherin subunit alpha-1

Gene names

    • Name
      Kpna1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    P83953

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00001207211-538Importin subunit alpha-5
Modified residue3Phosphothreonine
Modified residue63Phosphoserine

Post-translational modification

Polyubiquitinated in the presence of RAG1 (in vitro).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed significantly in the heart, kidney, placenta, pancreas and spleen with lower levels detected in the brain, liver and skeletal muscle.

Induction

Injection of 6-hydroxydopamine into the ascending medial forebrain bundle causes the formation of lesions and leads to up-regulation of KPNA1 in the denervated striatum in a time-dependent manner, reaching a maximum two weeks post-lesion.

Interaction

Subunit

Heterodimer; with KPNB1 (By similarity).
Interacts with APEX1 (via its N-terminus) (By similarity).
Interacts with CTNNBL1 (via its N-terminal) (By similarity).
Interacts with AICDA (via its NLS) (By similarity).
Interacts with ANP32E and ZIC3 (By similarity).
Interacts with SNAI1 (via zinc fingers) (By similarity).
Interacts with NSMF; the interaction occurs in a calcium-independent manner after synaptic NMDA receptor stimulation and is required for nuclear import of NSMF but is competed by CABP1 (PubMed:18303947).
Interacts with DCAF8 (By similarity).
Interacts with ITSN1 isoform 2 (By similarity).
Interacts with TALDO1 (By similarity).
Interacts with the AMPK-mediated 'Ser-659' phosphorylated form of ACSS2; this interaction results in nuclear translocation of ACSS2 (By similarity).
Interacts with BAP1 (via C-terminus); the interaction contributes to BAP1 nuclear localization (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain, compositional bias, motif, repeat.

TypeIDPosition(s)Description
Region1-41Disordered
Domain1-57IBB
Compositional bias10-41Basic and acidic residues
Motif42-51Nuclear localization signal
Repeat77-117ARM 1; truncated
Repeat118-161ARM 2
Region149-241NLS binding site (major)
Repeat162-206ARM 3
Repeat207-245ARM 4
Region245-437Binding to RAG1
Repeat246-290ARM 5
Repeat291-330ARM 6
Region318-406NLS binding site (minor)
Repeat331-372ARM 7
Repeat373-412ARM 8
Repeat413-457ARM 9
Repeat460-504ARM 10; atypical

Domain

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).

Sequence similarities

Belongs to the importin alpha family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    538
  • Mass (Da)
    60,137
  • Last updated
    2004-06-07 v1
  • Checksum
    B5B66B860D96EC5B
MSTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEAQINNMEMAPGGVITSDMTEMIFSNSPEQQLSATQKFRKLLSKEPNPPIDEVINTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWTISNITAGNRAQIQTVIDANMFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDAKIVQVALNGLENILRLGEQEAKRNGSGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDLSQQQYIFQQCEAPMEGSQL

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I6AKK8A0A8I6AKK8_RATKpna1547
A0A0G2K0T0A0A0G2K0T0_RATKpna1544
A0A8I6A8S8A0A8I6A8S8_RATKpna1145
Q56R20Q56R20_RATKpna1538

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias10-41Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY351984
EMBL· GenBank· DDBJ
AAQ56727.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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