P83886 · ASAL_ALLSA

  • Protein
    Mannose-specific lectin
  • Gene
    LECASAL
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Mannose-specific lectin (PubMed:17265166, PubMed:9037141).
Shows agglutinating activity towards rabbit erythrocytes (PubMed:35398454, PubMed:9037141).
However, it does not show agglutinating activity towards human erythrocytes (PubMed:9037141).
Has insecticidal activity against the cotton leafworm S.littoralis and the peach potato aphid M.persicae (PubMed:17147631, PubMed:17265166).
Also displays antiviral activity and therefore may contribute to defense against infections (PubMed:35398454).

Miscellaneous

Has an inhibitory effect on SARS-CoV-2 in Vero E6 cells (IC50=4 ug/ml) (PubMed:35398454).
Viral replication is inhibited by 80% at a concentration of 20 ug/ml, and 50% inhibition is observed at concentrations of 5 ug/ml and 10 ug/ml (PubMed:35398454).

Features

Showing features for binding site.

118120406080100120140160180
TypeIDPosition(s)Description
Binding site56alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site58alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site60alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site64alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site71alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site72alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site74alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site88alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site90alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site92alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site96alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site103alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site104alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site107alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site114alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site120alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site122alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site124alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site128alpha-D-mannopyranose (UniProtKB | ChEBI)
Binding site133alpha-D-mannopyranose (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular FunctionD-mannose binding
Molecular Functionprotein homodimerization activity
Biological Processcell-cell adhesion
Biological Processdefense response to insect
Biological Processdefense response to virus
Biological Processregulation of defense response to virus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Mannose-specific lectin
  • Alternative names
    • ASAL
    • ASARI
    • Allimin
    • Leaf agglutinin
    • Root agglutinin

Gene names

    • Name
      LECASAL
    • Synonyms
      LECASARI

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Asparagales > Amaryllidaceae > Allioideae > Allieae > Allium

Accessions

  • Primary accession
    P83886
  • Secondary accessions
    • P92930
    • P92931
    • Q19K88
    • Q5I1X6
    • Q679P1

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-30
ChainPRO_000006456431-181Mannose-specific lectin
Disulfide bond59↔83

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain31-140Bulb-type lectin

Keywords

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

P83886-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    181
  • Mass (Da)
    19,282
  • Last updated
    2008-01-15 v3
  • Checksum
    4D89A957F75A753D
MGRTTSSPKAMMRIATVAAILTILASTCMARNVLTNGEGLYAGQSLDVEQYKFIMQDDCNLVLYEYSTPIWASNTGVTGKNGCRAVMQRDGNFVVYDVNGRPVWASNSVRGNGNYILVLQKDRNVVIYGSDIWSTGTYRRSVGGAVVMAMNGTVDGGSVIGPVVVNQNVTAAIRKVGTGAA

P83886-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence AAW48531.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict3in Ref. 2; ABF70332
Sequence conflict32-34in Ref. 5; AA sequence
Sequence conflict33in Ref. 1; AAB64237, 3; AAW48531 and 4; AAR23523
Sequence conflict47in Ref. 1; AA sequence
Sequence conflict56-59in Ref. 5; AA sequence
Sequence conflict98in Ref. 6; AA sequence
Sequence conflict114in Ref. 4; AAR23523
Sequence conflict118in Ref. 1; AAB64237 and 4; AAR23523
Sequence conflict121in Ref. 1; AAB64237 and 4; AAR23523
Alternative sequenceVSP_030211138-148in isoform 2
Alternative sequenceVSP_030212149-181in isoform 2
Sequence conflict168-169in Ref. 2; ABF70332
Sequence conflict178in Ref. 1; AAB64237

Mass Spectrometry

Molecular mass is 12,382 Da. Determined by MALDI. Leaf agglutinin.
Molecular mass is 15,682 Da. Determined by MALDI. Root agglutinin.
Molecular mass is 12,500 Da. Determined by Electrospray. Leaf agglutinin.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U58947
EMBL· GenBank· DDBJ
AAB64237.1
EMBL· GenBank· DDBJ
mRNA
U58948
EMBL· GenBank· DDBJ
AAB64238.1
EMBL· GenBank· DDBJ
mRNA
DQ525625
EMBL· GenBank· DDBJ
ABF70332.1
EMBL· GenBank· DDBJ
mRNA
AY866499
EMBL· GenBank· DDBJ
AAW48531.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AY376827
EMBL· GenBank· DDBJ
AAR23523.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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