P83469 · DIS_MACLO

Function

function

Is a potent and selective inhibitor of alpha-1/beta-1 (ITGA1/ITGB1) integrin. It blocks the adhesion of alpha-1/beta-1-expressing K562 cells to immobilized collagens IV and I with IC50 of 2 and 0.5 nM, respectively. Potently inhibits angiogenesis in chicken and in mouse model and reduces tumor development by half. Is 25-fold less potent than viperistatin.

Miscellaneous

Negative results: does not show inhibitory activity toward alpha-2/beta-1 (ITGA2/ITGB1), alpha-IIb/beta-3 (ITGA2B/ITGB3), alpha-V/beta-3 (ITGAV/ITGB3), alpha-4/beta-1 (ITGA4/ITGB1), alpha-5/beta-1 (ITGA5/ITGB1), alpha-6/beta-1 (ITGA6/ITGB1), and alpha-9/beta-1 (ITGA9/ITGB1), alpha-4/beta-7 (ITGA4/ITGB7) integrins.

Features

Showing features for site.

141510152025303540
CTTGPCCRQCKLKPAGTTCWKTSLTSHYCTGKSCDCPLYPG
TypeIDPosition(s)Description
Site22Indirectly maintains the active conformation of the loop

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functiontoxin activity
Biological Processangiogenesis
Biological Processcell differentiation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Disintegrin obtustatin

Organism names

Accessions

  • Primary accession
    P83469

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis24Increase in inhibitory activity towards alpha-1/beta-1 integrin.

PTM/Processing

Features

Showing features for disulfide bond, chain.

TypeIDPosition(s)Description
Disulfide bond1↔10
ChainPRO_00001018141-41Disintegrin obtustatin
Disulfide bond6↔29
Disulfide bond7↔34
Disulfide bond19↔36

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain1-41Disintegrin
Motif21-23Cell attachment site; atypical (KTS)

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    41
  • Mass (Da)
    4,401
  • Last updated
    2002-11-01 v1
  • Checksum
    AC81277E3E8DD536
CTTGPCCRQCKLKPAGTTCWKTSLTSHYCTGKSCDCPLYPG

Keywords

Similar Proteins

Disclaimer

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