P83257 · T3D1B_PIRLC
- ProteinDelta-amaurobitoxin-Pl1b
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Insecticidal toxin. Binds to site 4 of insect voltage-gated sodium channel (Nav) and inhibits channel inactivation. In vivo, it lethal to lepidopteran larvae. Has no adverse affects when intracerebroventricularly injected in mice at a dose of 0.2 ug, but causes reversible paralysis of legs when injected intracerebroventricularly in mice at a dose of 2.0 ug.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 8 | Pharmacophore | ||||
Sequence: R | ||||||
Site | 12 | Pharmacophore | ||||
Sequence: W | ||||||
Site | 19 | May be involved in voltage sensor trapping upon activation of sodium channel | ||||
Sequence: D | ||||||
Site | 22 | Pharmacophore | ||||
Sequence: Y | ||||||
Site | 24 | Pharmacophore | ||||
Sequence: S | ||||||
Site | 26 | Pharmacophore | ||||
Sequence: R | ||||||
Site | 28 | Pharmacophore | ||||
Sequence: M | ||||||
Site | 30 | Pharmacophore | ||||
Sequence: Y | ||||||
Site | 32 | Pharmacophore | ||||
Sequence: R | ||||||
Site | 34 | Pharmacophore | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | sodium channel regulator activity | |
Molecular Function | toxin activity | |
Biological Process | modulation of process of another organism |
Keywords
- Molecular function
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDelta-amaurobitoxin-Pl1b
- Short namesDelta-AMATX-Pl1b
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Araneae > Araneomorphae > Entelegynae > Agelenidae > Pireneitega
Accessions
- Primary accessionP83257
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Toxic dose
LD50 is 24.2 ng/mg body weight of lepidoptera larvae.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 3 | No change in binding affinity and in lethal dose. | ||||
Sequence: V → A | ||||||
Mutagenesis | 5 | No change in binding affinity and in lethal dose. | ||||
Sequence: D → A | ||||||
Mutagenesis | 7 | No change in binding affinity and in lethal dose. | ||||
Sequence: Q → A | ||||||
Mutagenesis | 8 | 51-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: R → A | ||||||
Mutagenesis | 8 | More than 80-fold decrease in binding affinity and 5-fold decrease in toxicity. | ||||
Sequence: R → D | ||||||
Mutagenesis | 11 | No change in binding affinity and in lethal dose. | ||||
Sequence: S → A | ||||||
Mutagenesis | 12 | More than 160-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: W → A | ||||||
Mutagenesis | 12 | 13.5-fold decrease in binding affinity and more than 8-fold decrease in toxicity. | ||||
Sequence: W → F | ||||||
Mutagenesis | 13 | No change in binding affinity and in lethal dose. | ||||
Sequence: S → A | ||||||
Mutagenesis | 16 | No change in binding affinity and in lethal dose. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 19 | No change in binding affinity and 2.9-fold decrease in toxicity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 21 | No change in binding affinity and in lethal dose. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 22 | 54-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 24 | 40-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: S → A | ||||||
Mutagenesis | 26 | 16-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: R → A | ||||||
Mutagenesis | 28 | 28-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: M → A | ||||||
Mutagenesis | 30 | 58-fold decrease in binding affinity and 5.1-fold decrease in toxicity. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 30 | 16-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 32 | 24-fold decrease in binding affinity and 2.3-fold decrease in toxicity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 34 | 18-fold decrease in binding affinity and no change in lethal dose. | ||||
Sequence: R → A | ||||||
Mutagenesis | 35 | No change in binding affinity and in lethal dose. | ||||
Sequence: N → A | ||||||
Mutagenesis | 36 | No change in binding affinity and in lethal dose. | ||||
Sequence: N → A | ||||||
Mutagenesis | 37 | No change in binding affinity and in lethal dose. | ||||
Sequence: S → A |
PTM/Processing
Features
Showing features for peptide, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Peptide | PRO_0000044962 | 1-37 | Delta-amaurobitoxin-Pl1b | |||
Sequence: ACVGDGQRCASWSGPYCCDGYYCSCRSMPYCRCRNNS | ||||||
Disulfide bond | 2↔18 | |||||
Sequence: CVGDGQRCASWSGPYCC | ||||||
Disulfide bond | 9↔23 | |||||
Sequence: CASWSGPYCCDGYYC | ||||||
Disulfide bond | 17↔33 | |||||
Sequence: CCDGYYCSCRSMPYCRC | ||||||
Disulfide bond | 25↔31 | |||||
Sequence: CRSMPYC | ||||||
Modified residue | 37 | Serine amide | ||||
Sequence: S |
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Domain
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Sequence similarities
Belongs to the neurotoxin 07 (Beta/delta-agtx) family. 02 (aga-3) subfamily.
Keywords
- Domain