P81066 · IRX2_MOUSE
- ProteinIroquois-class homeodomain protein IRX-2
- GeneIrx2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids474 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 115-177 | Homeobox; TALE-type | ||||
Sequence: DPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKEN |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | DNA-binding transcription repressor activity, RNA polymerase II-specific | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Biological Process | cell development | |
Biological Process | metanephros development | |
Biological Process | neuron differentiation | |
Biological Process | proximal/distal pattern formation involved in metanephric nephron development | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | specification of loop of Henle identity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameIroquois-class homeodomain protein IRX-2
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP81066
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000049154 | 1-474 | Iroquois-class homeodomain protein IRX-2 | |||
Sequence: MSYPQGYLYQAPGSLALYSCPAYGASALAAPRSEELARSASGSAFSPYPGSAAFTAQAATGFGSPLQYSADAAAAAAAGFPSYVGSPYDTHTTGMTGAISYHPYGSAAYPYQLNDPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWAPRNKSEDEDEDEGDASRSKEESSDKAQDGTETSAEDEGISLHVDSLTDHSCSAESDGEKLPCRAGDALCESGSECKDKFEDLEDEEDEEDECERDLAPPKPVTSSPLTGVEAPLLSPAPEAAPRGGSGGKTPLGSRTSPGAPPPASKPKLWSLAEIATSDLKQPSLGPGCGPPGLPAAAAPASTGAPPGGSPYSASPLLGRHLYYTSPFYGNYTNYGNLNAALQGQGLLRYNTAASSPGETLHAMPKAASDTGKAGSHSLESHYRPPGGGYEPKKDTSEGCAVVGAGVQTYL | ||||||
Modified residue | 187 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in specific and overlapping patterns with Irx1 and Irx3 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule.
Developmental stage
First expressed at 8.0 dpc. During neural tube closure (8.5 dpc), expression appears for the first time in the rhombencephalon in the presumptive region of future rhombomere 4. During neurogenesis (9.5 dpc to 10.5 dpc), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon and rhombencephalon. Expression is strong in the tectum of the mesencephalon and in the hindbrain, expression is restricted to rhombomeres. Expression in the spinal cord is weak and confined to the alar plate. Beginning at 9.5 dpc, expressed in the epithelial component of the branchial arches and foregut. At 10.5 dpc, expression extends rostrally into the dorsal diencephalon. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from 10.5 dpc onwards. From 10.5 dpc onwards, weak expression begins in the limb bud. Also expressed in other tissues during organogenesis; at 9.5 dpc, expressed in the superficial ectoderm surrounding the body and in the region of the foregut, which will form the pharynx and the lung bud. at 10.5 dpc, found in the cephalic mesenchyme around the optic vesicle. By 12.5 dpc, still expressed in the mesenchyme, and expression begins in specific subsets of post-mitotic cells in the neuroretina. As development ensues, expression increases in the neuroretina and mesenchymal expression gradually decreases. At 16.5 dpc, expressed exclusively in the inner neuroblast layers of the neuroretina. Expressed in the developing heart in the ventricular septum from the onset of its formation (10.5 dpc) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 177-220 | Disordered | ||||
Sequence: NKMTWAPRNKSEDEDEDEGDASRSKEESSDKAQDGTETSAEDEG | ||||||
Compositional bias | 198-220 | Basic and acidic residues | ||||
Sequence: SRSKEESSDKAQDGTETSAEDEG | ||||||
Region | 262-373 | Disordered | ||||
Sequence: EDLEDEEDEEDECERDLAPPKPVTSSPLTGVEAPLLSPAPEAAPRGGSGGKTPLGSRTSPGAPPPASKPKLWSLAEIATSDLKQPSLGPGCGPPGLPAAAAPASTGAPPGGS | ||||||
Region | 420-461 | Disordered | ||||
Sequence: PGETLHAMPKAASDTGKAGSHSLESHYRPPGGGYEPKKDTSE |
Sequence similarities
Belongs to the TALE/IRO homeobox family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length474
- Mass (Da)49,484
- Last updated2002-04-16 v2
- Checksum04E39C42E717F98B
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 27-33 | in Ref. 2; AAF63956 | ||||
Sequence: ALAAPRS → GGARA | ||||||
Sequence conflict | 185-186 | in Ref. 5 | ||||
Sequence: NK → EQ | ||||||
Compositional bias | 198-220 | Basic and acidic residues | ||||
Sequence: SRSKEESSDKAQDGTETSAEDEG |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF295369 EMBL· GenBank· DDBJ | AAG10083.1 EMBL· GenBank· DDBJ | mRNA | ||
AF165986 EMBL· GenBank· DDBJ | AAF63956.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK148520 EMBL· GenBank· DDBJ | BAE28598.1 EMBL· GenBank· DDBJ | mRNA | ||
BC029750 EMBL· GenBank· DDBJ | AAH29750.1 EMBL· GenBank· DDBJ | mRNA | ||
Y15000 EMBL· GenBank· DDBJ | CAA75232.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. |