P81054 · PLMP_GRIFR

Function

Catalytic activity

  • Preferential cleavage in proteins: -Xaa-|-Lys- (in which Xaa may be Pro).
    EC:3.4.24.20 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Activity regulation

Inhibited by chelating agents such as EDTA and 1,10-phenanthroline.

pH Dependence

Optimum pH is 9.5. Active from pH 6 to pH 10.5. Stable from pH 5 to pH 10.

Temperature Dependence

Thermostable for 3 hours up to 80 degrees Celsius.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site298Zn2+ (UniProtKB | ChEBI); catalytic
Active site299
Binding site302Zn2+ (UniProtKB | ChEBI); catalytic
Binding site311Zn2+ (UniProtKB | ChEBI); catalytic
Site314Transition state stabilizer

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionmetal ion binding
Molecular Functionmetalloendopeptidase activity
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Peptidyl-Lys metalloendopeptidase
  • EC number
  • Short names
    MEP
  • Alternative names
    • GfMEP

Gene names

    • Name
      MEP

Organism names

Accessions

  • Primary accession
    P81054
  • Secondary accessions
    • Q8WZH2

Subcellular Location

Secreted
Note: Binds strongly to beta-1,3-glucan and chitin, major polysaccharides constituting the fungal cell wall.

Keywords

PTM/Processing

Features

Showing features for signal, propeptide, chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
Signal1-18
PropeptidePRO_000004339919-181
ChainPRO_0000043400182-348Peptidyl-Lys metalloendopeptidase
Disulfide bond186↔256
Glycosylation223O-linked (Man) threonine; partial
Disulfide bond258↔278

Keywords

PTM databases

Family & Domains

Sequence similarities

Belongs to the peptidase M35 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    348
  • Mass (Da)
    36,879
  • Last updated
    2005-12-06 v2
  • Checksum
    5F3859F10B423C79
MFSSVMVALVSLAVAVSANPGLSLKVSGPEAVDGVNNLKVVTTITNTGDETLKLLNDPRGALHTMPTDTFAITNESGETPSFIGVKVKYVPSMAAKSTGENVFAVIAPGQSVNVEHDLSAAYNFTSSGAGTYALEALNVFNYIDPETNEPVEIWADAEAHTTAVSGKLAVVRATPTLTRPVTYNGCSSSEQSALAAAASAAQSYVAESLSYLQTHTAATPRYTTWFGSYISSRHSTVLQHYTDMNSNDFSSYSFDCTCTAAGTFAYVYPNRFGTVYLCGAFWKAPTTGTDSQAGTLVHESSHFTRNGGTKDYAYGQAAAKSLATMDPDKAVMNADNHEYFSENNPAQS

Mass Spectrometry

Molecular mass is 18,028 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB076805
EMBL· GenBank· DDBJ
BAB82381.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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