P77667 · SUFA_ECOLI

Function

function

Member of gene cluster sufABCDSE that mediates iron-sulfur cluster assembly under oxidative stress and iron limitation conditions (PubMed:17941825).
Binds [2Fe-2S] and [4Fe-4S] clusters by mobilizing sulfur atoms provided by the SufS-SufE cysteine desulfurase system and then transfers the assembled Fe-S clusters to target proteins including ferredoxin and aconitase (PubMed:17350000, PubMed:19366265).
Seems to act as a Fe-S cluster carrier rather than a scaffold, this role being performed by SufB and SufC (PubMed:19810706, PubMed:23018275).

Features

Showing features for binding site.

1122102030405060708090100110120
TypeIDPosition(s)Description
Binding site50[2Fe-2S] cluster (UniProtKB | ChEBI); sulfuration is probably achieved via an internal sulfur transfer from C-114 or C-116
Binding site50[4Fe-4S] cluster (UniProtKB | ChEBI); sulfuration is probably achieved via an internal sulfur transfer from C-114 or C-116
Binding site114[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site114[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site116[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site116[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentiron-sulfur cluster transfer complex
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionprotein homodimerization activity
Biological Processiron-sulfur cluster assembly
Biological Processprotein maturation by iron-sulfur cluster transfer
Biological Processresponse to oxidative stress

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Iron-sulfur cluster assembly protein SufA

Gene names

    • Name
      sufA
    • Synonyms
      ydiC
    • Ordered locus names
      b1684, JW1674

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P77667

Proteomes

Phenotypes & Variants

Disruption phenotype

Deletion has only a mild effect on cell growth (PubMed:17941825).
However, deletion of both IscA and SufA results in a severe growth phenotype in minimal medium under aerobic growth conditions (PubMed:17941825).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis50Decrease of sulfur binding activity.
Mutagenesis114Strong decrease of sulfur binding activity.
Mutagenesis116Strong decrease of sulfur binding activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000769871-122Iron-sulfur cluster assembly protein SufA

Proteomic databases

Interaction

Subunit

Homodimer (PubMed:16298366).
Interacts with SufB and SufC (PubMed:19810706).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY P77667sufB P775225EBI-1125011, EBI-562758
BINARY P77667sufC P774995EBI-1125011, EBI-561601

Complex viewer

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the HesB/IscA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    122
  • Mass (Da)
    13,300
  • Last updated
    1997-02-01 v1
  • MD5 Checksum
    03AE0C2783E02563669BDFA97CA19A67
MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLGVKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQIFKFHNPKAQNECGCGESFGV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U00096
EMBL· GenBank· DDBJ
AAC74754.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA15453.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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