P77399 · FADJ_ECOLI

Function

function

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids.

Catalytic activity

Pathway

Lipid metabolism; fatty acid beta-oxidation.

Features

Showing features for site.

TypeIDPosition(s)Description
Site118Important for catalytic activity
Site140Important for catalytic activity

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentfatty acid beta-oxidation multienzyme complex
Molecular Function3-hydroxyacyl-CoA dehydrogenase activity
Molecular Function3-hydroxybutyryl-CoA epimerase activity
Molecular Functionenoyl-CoA hydratase activity
Molecular Functionlong-chain-3-hydroxyacyl-CoA dehydrogenase activity
Molecular FunctionNAD+ binding
Biological Processfatty acid beta-oxidation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Fatty acid oxidation complex subunit alpha

Including 2 domains:

  • Recommended name
    3-hydroxyacyl-CoA dehydrogenase
  • EC number

Gene names

    • Name
      fadJ
    • Synonyms
      yfcX
    • Ordered locus names
      b2341, JW2338

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P77399
  • Secondary accessions
    • Q6KCX2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001092981-714Fatty acid oxidation complex subunit alpha

Proteomic databases

Expression

Induction

Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.

Interaction

Subunit

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P77399yfdH P772932EBI-545361, EBI-545379
View interactors in UniProtKB
View CPX-3966 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-190Enoyl-CoA hydratase
Region306-7143-hydroxyacyl-CoA dehydrogenase

Sequence similarities

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    714
  • Mass (Da)
    77,072
  • Last updated
    1997-02-01 v1
  • Checksum
    F4E0A75680039A0D
MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U00096
EMBL· GenBank· DDBJ
AAC75401.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA16195.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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