P77338 · MSCK_ECOLI
- ProteinMechanosensitive channel MscK
- GenemscK
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1120 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Mechanosensitive channel that opens in response to membrane tension and specific ionic conditions. Requires high concentrations of external K+, NH4+, Rb+ or Cs+ to gate. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Forms an ion channel of 1.0 nanosiemens conductance. The channel can remain active for between 30 seconds and over 3 minutes; it does not desensitize upon extended pressure. Its activity is masked in wild-type cells by the MscS channel.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | mechanosensitive monoatomic ion channel activity | |
Biological Process | intracellular water homeostasis | |
Biological Process | potassium ion transport | |
Biological Process | response to potassium ion |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMechanosensitive channel MscK
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP77338
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 34-499 | Periplasmic | ||||
Sequence: RASSNGDLPTKADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKMRQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVSLQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQRKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPDEAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKEQIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAFVNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSVSKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKA | ||||||
Transmembrane | 500-520 | Helical | ||||
Sequence: WPAVFIAFLAGLPLLLIAGLI | ||||||
Topological domain | 521-560 | Cytoplasmic | ||||
Sequence: HWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRA | ||||||
Transmembrane | 561-581 | Helical | ||||
Sequence: LPVCLIILAVGLILLTMQLNI | ||||||
Topological domain | 582 | Periplasmic | ||||
Sequence: S | ||||||
Transmembrane | 583-603 | Helical | ||||
Sequence: ELLWSFSKKLAIFWLVFGLCW | ||||||
Topological domain | 604-634 | Cytoplasmic | ||||
Sequence: KVLEKNGVAVRHFGMPEQQTSHWRRQIVRIS | ||||||
Transmembrane | 635-655 | Helical | ||||
Sequence: LALLPIHFWSVVAELSPLHLM | ||||||
Topological domain | 656-657 | Periplasmic | ||||
Sequence: DD | ||||||
Transmembrane | 658-678 | Helical | ||||
Sequence: VLGQAMIFFNLLLIAFLVWPM | ||||||
Topological domain | 679-692 | Cytoplasmic | ||||
Sequence: CRESWRDKESHTMR | ||||||
Transmembrane | 693-713 | Helical | ||||
Sequence: LVTITVLSIIPIALMVLTATG | ||||||
Topological domain | 714-728 | Periplasmic | ||||
Sequence: YFYTTLRLAGRWIET | ||||||
Transmembrane | 729-749 | Helical | ||||
Sequence: VYLVIIWNLLYQTVLRGLSVA | ||||||
Topological domain | 750-796 | Cytoplasmic | ||||
Sequence: ARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITML | ||||||
Transmembrane | 797-817 | Helical | ||||
Sequence: LMFALFGVMFWAIWSDLITVF | ||||||
Topological domain | 818-839 | Periplasmic | ||||
Sequence: SYLDSITLWHYNGTEAGAAVVK | ||||||
Transmembrane | 840-860 | Helical | ||||
Sequence: NVTMGSLLFAIIASMVAWALI | ||||||
Topological domain | 861-886 | Cytoplasmic | ||||
Sequence: RNLPGLLEVLVLSRLNMRQGASYAIT | ||||||
Transmembrane | 887-907 | Helical | ||||
Sequence: TILNYIIIAVGAMTVFGSLGV | ||||||
Topological domain | 908-921 | Periplasmic | ||||
Sequence: SWDKLQWLAAALSV | ||||||
Transmembrane | 922-942 | Helical | ||||
Sequence: GLGFGLQEIFGNFVSGLIILF | ||||||
Topological domain | 943-1120 | Cytoplasmic | ||||
Sequence: ERPVRIGDTVTIGSFSGTVSKIRIRATTITDFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHPRVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAFNQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 922 | Prevents growth in medium containing high levels of potassium in the presence of betaine. | ||||
Sequence: G → S |
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-33 | |||||
Sequence: MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFA | ||||||
Chain | PRO_0000043366 | 34-1120 | Mechanosensitive channel MscK | |||
Sequence: RASSNGDLPTKADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKMRQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVSLQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQRKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPDEAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKEQIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAFVNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSVSKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFLAGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLIILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTSHWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRESWRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQTVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLMFALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVAWALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQWLAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTITDFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHPRVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAFNQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG |
Proteomic databases
Structure
Family & Domains
Features
Showing features for coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 43-98 | |||||
Sequence: TKADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEA | ||||||
Coiled coil | 126-266 | |||||
Sequence: STLSLRQLETRVAQALDDLQNAQNDLASYNSQLVSLQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQRKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTE | ||||||
Coiled coil | 360-422 | |||||
Sequence: DELENMTNRIADLRLEQFEVNQQRDALFQSDAFVNKLEEGHTNEVNSEVHDALLQVVDMRREL | ||||||
Coiled coil | 1057-1081 | |||||
Sequence: YVRELRDRSRTVDELNRTIDQLCRE |
Sequence similarities
Belongs to the MscS (TC 1.A.23) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length1,120
- Mass (Da)127,215
- Last updated1997-02-01 v1
- Checksum809895660D2BD444
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y07802 EMBL· GenBank· DDBJ | CAA69140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U82664 EMBL· GenBank· DDBJ | AAB40219.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC73567.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76244.1 EMBL· GenBank· DDBJ | Genomic DNA |