P77165 · PAOA_ECOLI

  • Protein
    Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA
  • Gene
    paoA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Oxidizes aldehydes to the corresponding carboxylic acids with a preference for aromatic aldehydes. It might play a role in the detoxification of aldehydes to avoid cell damage.

Catalytic activity

Cofactor

[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 2 [2Fe-2S] clusters.

Activity regulation

The complex requires PaoD for activity.

Features

Showing features for binding site.

122920406080100120140160180200220
TypeIDPosition(s)Description
Binding site99[2Fe-2S] cluster 1 (UniProtKB | ChEBI)
Binding site104[2Fe-2S] cluster 1 (UniProtKB | ChEBI)
Binding site105[2Fe-2S] cluster 1 (UniProtKB | ChEBI)
Binding site107[2Fe-2S] cluster 1 (UniProtKB | ChEBI)
Binding site119[2Fe-2S] cluster 1 (UniProtKB | ChEBI)
Binding site158[2Fe-2S] cluster 2 (UniProtKB | ChEBI)
Binding site161[2Fe-2S] cluster 2 (UniProtKB | ChEBI)
Binding site208[2Fe-2S] cluster 2 (UniProtKB | ChEBI)
Binding site210[2Fe-2S] cluster 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentouter membrane-bounded periplasmic space
Cellular Componentoxidoreductase complex
Cellular Componentperiplasmic space
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Functioncarboxylate reductase activity
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity, acting on the aldehyde or oxo group of donors
Biological Processcatabolic process
Biological Processcellular detoxification of aldehyde

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA
  • EC number

Gene names

    • Name
      paoA
    • Synonyms
      yagT
    • Ordered locus names
      b0286, JW0280

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P77165
  • Secondary accessions
    • Q2MCD6

Proteomes

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Mutation results in complete impairment of cell growth in the presence of cinnamaldehyde.

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-53Tat-type signal
ChainPRO_000018941454-229Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA

Post-translational modification

Exported by the Tat system (PubMed:17218314).
The position of the signal peptide cleavage has not been experimentally proven (Probable)

Proteomic databases

Interaction

Subunit

Heterotrimer composed of PaoA, PaoB and PaoC.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P77165proX P0AFM22EBI-1115563, EBI-1129961
View interactors in UniProtKB
View CPX-4281 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-21Disordered
Domain61-1372Fe-2S ferredoxin-type

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    229
  • Mass (Da)
    24,343
  • Last updated
    1997-02-01 v1
  • Checksum
    216961D7BD21836C
MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDTLRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYCTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U73857
EMBL· GenBank· DDBJ
AAB18015.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC73389.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE76070.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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