P76474 · ARNF_ECOLI
- ProteinProbable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF
- GenearnF
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids128 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the periplasmic side of the inner membrane.
Pathway
Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | carbohydrate derivative transmembrane transporter activity | |
Molecular Function | transmembrane transporter activity | |
Biological Process | carbohydrate derivative transport | |
Biological Process | lipid A biosynthetic process | |
Biological Process | lipopolysaccharide biosynthetic process | |
Biological Process | response to iron(III) ion | |
Biological Process | transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProbable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF
- Short namesL-Ara4N-phosphoundecaprenol flippase subunit ArnF
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP76474
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-2 | Cytoplasmic | ||||
Sequence: MG | ||||||
Transmembrane | 3-23 | Helical | ||||
Sequence: LMWGLFSVIIASVAQLSLGFA | ||||||
Topological domain | 24-35 | Periplasmic | ||||
Sequence: ASHLPPMTHLWD | ||||||
Transmembrane | 36-56 | Helical | ||||
Sequence: FIAALLAFGLDARILLLGLLG | ||||||
Topological domain | 57-76 | Cytoplasmic | ||||
Sequence: YLLSVFCWYKTLHKLALSKA | ||||||
Transmembrane | 77-97 | Helical | ||||
Sequence: YALLSMSYVLVWIASMVLPGW | ||||||
Topological domain | 98-100 | Periplasmic | ||||
Sequence: EGT | ||||||
Transmembrane | 101-121 | Helical | ||||
Sequence: FSLKALLGVACIMSGLMLIFL | ||||||
Topological domain | 122-128 | Cytoplasmic | ||||
Sequence: PTTKQRY |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene are polymyxin sensitive. Lipid A is no longer modified with L-Ara4N even though the level of the lipid-linked donor of the L-Ara4N moiety, alpha-L-Ara4N-phosphoundecaprenol, is not reduced. However, the alpha-L-Ara4N-phosphoundecaprenol is less concentrated on the periplasmic surface of the inner membrane when compared to wild-type.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000218152 | 1-128 | Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF | |||
Sequence: MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFGLDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFSLKALLGVACIMSGLMLIFLPTTKQRY |
Proteomic databases
Expression
Induction
Induced by BasR.
Structure
Sequence
- Sequence statusComplete
- Length128
- Mass (Da)14,085
- Last updated2002-02-21 v2
- Checksum1ACEEE17FDF6B446
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L35031 EMBL· GenBank· DDBJ | AAB04894.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
U00096 EMBL· GenBank· DDBJ | AAC75318.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76676.1 EMBL· GenBank· DDBJ | Genomic DNA |