P76108 · YDCS_ECOLI
- ProteinBifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding protein
- GeneydcS
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids381 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the formation of short polymers of R-3-hydroxybutyrate (cPHB) (PubMed:18640095).
Involved in natural transformation (PubMed:26826386).
Probably part of the ABC transporter complex YdcSTUV. During natural transformation, may bind dsDNA and convey it to the inner membrane channel formed by YdcV (Probable)
Involved in natural transformation (PubMed:26826386).
Probably part of the ABC transporter complex YdcSTUV. During natural transformation, may bind dsDNA and convey it to the inner membrane channel formed by YdcV (Probable)
Catalytic activity
- (3R)-3-hydroxybutanoyl-CoA + [(3R)-hydroxybutanoate](n) = [(3R)-hydroxybutanoate](n+1) + CoA
CHEBI:57315 + [(3R)-hydroxybutanoate](n) RHEA-COMP:14464 = [(3R)-hydroxybutanoate](n+1) RHEA-COMP:14465 + CHEBI:57287
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.14 mM | 3-hydroxybutyryl-CoA |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
18.7 nmol/min/mg |
pH Dependence
Optimum pH is 6.8-7.8.
Temperature Dependence
Optimum temperature is 37 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing | |
Cellular Component | membrane | |
Cellular Component | outer membrane-bounded periplasmic space | |
Molecular Function | acyltransferase activity | |
Molecular Function | polyamine binding | |
Biological Process | DNA import into cell involved in transformation | |
Biological Process | poly-hydroxybutyrate biosynthetic process | |
Biological Process | polyamine transport | |
Biological Process | transmembrane transport |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding protein
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP76108
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MSKTFARSSLCALSMTIMTAHA | ||||||
Chain | PRO_0000031843 | 23-381 | Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding protein | |||
Sequence: AEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLIMGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPPDSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQYQAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFPKEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPLLGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR |
Proteomic databases
Expression
Induction
Induced under nitrogen-limited growth.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length381
- Mass (Da)42,295
- Last updated1997-02-01 v1
- ChecksumFC5072B0B52CFB05
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U00096 EMBL· GenBank· DDBJ | AAC74522.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76440.1 EMBL· GenBank· DDBJ | Genomic DNA |