P76077 · PAAA_ECOLI
- Protein1,2-phenylacetyl-CoA epoxidase, subunit A
- GenepaaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids309 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA.
Catalytic activity
- H+ + NADPH + O2 + phenylacetyl-CoA = 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
Cofactor
Pathway
Aromatic compound metabolism; phenylacetate degradation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 33 | substrate | ||||
Sequence: R | ||||||
Binding site | 37 | substrate | ||||
Sequence: Q | ||||||
Binding site | 103-106 | substrate | ||||
Sequence: KYSS | ||||||
Binding site | 132 | substrate | ||||
Sequence: N | ||||||
Binding site | 193 | substrate | ||||
Sequence: M | ||||||
Binding site | 202-204 | substrate | ||||
Sequence: SPN | ||||||
Binding site | 214 | substrate | ||||
Sequence: K | ||||||
Binding site | 218 | substrate | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | phenylacetyl-CoA 1,2-epoxidase complex | |
Molecular Function | phenylacetyl-CoA 1,2-epoxidase activity | |
Biological Process | phenylacetate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name1,2-phenylacetyl-CoA epoxidase, subunit A
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP76077
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 210 | in strain: W | ||||
Sequence: T → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000058159 | 1-309 | 1,2-phenylacetyl-CoA epoxidase, subunit A | |||
Sequence: MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIVGMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRMKYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQRQGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLTWKIKRFTNDELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQERLDAKRKAWEEGTWVREAALAHAQKQHARKVA |
Proteomic databases
Expression
Induction
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.
Interaction
Subunit
Forms a stable heterotetramer (dimer of heterodimers) with PaaC.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P76077 | paaC P76079 | 6 | EBI-1119536, EBI-1131666 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length309
- Mass (Da)35,499
- Last updated1997-02-01 v1
- Checksum83AEC6D7AC084073
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X97452 EMBL· GenBank· DDBJ | CAA66090.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
U00096 EMBL· GenBank· DDBJ | AAC74470.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76423.1 EMBL· GenBank· DDBJ | Genomic DNA |