P74250 · GPX1_SYNY3
- ProteinHydroperoxy fatty acid reductase gpx1
- Genegpx1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids169 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Hydroperoxy fatty acid reductase essential for the removal of lipid hydroperoxides under normal and stress conditions, leading to the protection of membrane integrity.
Catalytic activity
- a hydroperoxy polyunsaturated fatty acid + H+ + NADPH = a hydroxy polyunsaturated fatty acid + H2O + NADP+
Activity regulation
Mercaptosuccinate, pCMB, and nethylmaleimide act as inhibitors of the catalytic activity.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
83.1 μM | NADPH | |||||
215 μM | alpha-linolenic acid hydroperoxide |
pH Dependence
Optimum pH is 8.2.
Temperature Dependence
Optimum temperature is 37 degrees Celsius.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 41 | |||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | peroxidase activity | |
Biological Process | cellular response to oxidative stress |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameHydroperoxy fatty acid reductase gpx1
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Synechococcales > Merismopediaceae > Synechocystis
Accessions
- Primary accessionP74250
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000066663 | 1-169 | Hydroperoxy fatty acid reductase gpx1 | |||
Sequence: MTAQANNTIYGFSANALDGSPVALRDFEGKVLLIVNTASQCGFTPQYQGLQALYNRFGDRGFTVLGFPCNQFGQQEPGGSGEIKNFCETRYGVTFPLFEKVEVNGPNAHPLFKFLTAASPGMAIPFLGGAEDIKWNFTKFLVDRQGKVVKRYGSIAKPDEIAADIEKLL |
Proteomic databases
Expression
Induction
High light, methylviologen, t-butyl hydroperoxide, and salt stress conditions increase the expression level.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length169
- Mass (Da)18,452
- Last updated1997-02-01 v1
- Checksum0DC382089FCE39E2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000022 EMBL· GenBank· DDBJ | BAA18344.1 EMBL· GenBank· DDBJ | Genomic DNA |