P73257 · ARLY_SYNY3

Function

function

Catalyzes the last step of arginine biosynthesis, the conversion of argininosuccinate into L-arginine and fumarate.

Miscellaneous

A mutant overexpressing this enzyme grows faster than the wild-type strain under photoautotrophic conditions with 5 mM NaNO3 as a nitrogen source (PubMed:38478146).
In contrast, the growth of the mutant is similar under photoautotrophic conditions with 5 mM arginine as a nitrogen source (PubMed:38478146).

Catalytic activity

Activity regulation

Strongly inhibited by L-arginine (PubMed:35583703).
Inhibitory effects are lowered at pH 7.0 compared to those at pH 8.0 (PubMed:35583703).
At 45 degrees Celsius and pH 8.0, activity decreases to 94%, 74% and 37% in the presence of 0.6 mM, 2.8 mM and 10 mM arginine, respectively (PubMed:35583703).
Activity also decreases to 86% in the presence of 10 mM sodium succinate or sodium citrate (PubMed:35583703).
Activity does not decrease in the presence of 1 mM or 10 mM L-lysine, which has a similar structure to arginine (PubMed:35583703).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.182 mMargininosuccinate8.045
kcat is 6.78 sec-1 with argininosuccinate as substrate.

pH Dependence

Optimum pH is 8.0 (PubMed:35583703).
Shows more than 35% enzymatic activity in terms of relative activity at pH 7.5-9.5 (PubMed:35583703).

Temperature Dependence

Optimum temperature is 45 degrees Celsius (PubMed:35583703).
Shows more than 35% enzymatic activity in terms of relative activity at 20-50 degrees Celsius (PubMed:35583703).
Inactive above 55 degrees Celsius (PubMed:35583703).

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionargininosuccinate lyase activity
Biological Processarginine biosynthetic process via ornithine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Argininosuccinate lyase
  • EC number
  • Short names
    ASAL
  • Alternative names
    • Arginosuccinase
    • SyArgH

Gene names

    • Name
      argH
    • Ordered locus names
      slr1133

Organism names

Accessions

  • Primary accession
    P73257

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001378411-461Argininosuccinate lyase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    461
  • Mass (Da)
    50,948
  • Last updated
    1997-02-01 v1
  • Checksum
    B6E8C81B1CFB5FE2
MTKKTWSDRFEGTLHPAIALFNASIGFDIELIEYDLDGSIAHGKMLAKTGIISPGEAEQLVQGLEQIRQEYRAGNFNPGVDQEDVHFAVERRLTELVGDVGKKLHTARSRNDQVGTDVRLYLRAQIDDIRQRLRDFQAVLLQLAETNVETLIPGYTHLQRAQPVSLAHHLLAYFQMAQRDWQRLGEIRARTNVSPLGSGALAGTTFPIDRHYSAELLGFAGVYANSLDGVSDRDFAIEFLNAASLIMVHLSRLSEEMILWASQEFSFISLTDSCATGSSIMPQKKNPDVPELIRGKAGRVMGHLQGMLVLMKGLPLAYNKDLQEDKEALFDAVKTVQVSLEAMTILLDEGIVFRQERLAEAVAEDFSNATDVADYLAAKGVPFREAYNLVGKVVKTSLAAGKLLKDLTLAEWQALHPAFEEDIYQAITPQQVVAARNSYGGTGFEQVKMAIANAKAELSQT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000022
EMBL· GenBank· DDBJ
BAA17284.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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