P73257 · ARLY_SYNY3
- ProteinArgininosuccinate lyase
- GeneargH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids461 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the last step of arginine biosynthesis, the conversion of argininosuccinate into L-arginine and fumarate.
Miscellaneous
A mutant overexpressing this enzyme grows faster than the wild-type strain under photoautotrophic conditions with 5 mM NaNO3 as a nitrogen source (PubMed:38478146).
In contrast, the growth of the mutant is similar under photoautotrophic conditions with 5 mM arginine as a nitrogen source (PubMed:38478146).
In contrast, the growth of the mutant is similar under photoautotrophic conditions with 5 mM arginine as a nitrogen source (PubMed:38478146).
Catalytic activity
- 2-(N(omega)-L-arginino)succinate = fumarate + L-arginineThis reaction proceeds in the forward direction.
Activity regulation
Strongly inhibited by L-arginine (PubMed:35583703).
Inhibitory effects are lowered at pH 7.0 compared to those at pH 8.0 (PubMed:35583703).
At 45 degrees Celsius and pH 8.0, activity decreases to 94%, 74% and 37% in the presence of 0.6 mM, 2.8 mM and 10 mM arginine, respectively (PubMed:35583703).
Activity also decreases to 86% in the presence of 10 mM sodium succinate or sodium citrate (PubMed:35583703).
Activity does not decrease in the presence of 1 mM or 10 mM L-lysine, which has a similar structure to arginine (PubMed:35583703).
Inhibitory effects are lowered at pH 7.0 compared to those at pH 8.0 (PubMed:35583703).
At 45 degrees Celsius and pH 8.0, activity decreases to 94%, 74% and 37% in the presence of 0.6 mM, 2.8 mM and 10 mM arginine, respectively (PubMed:35583703).
Activity also decreases to 86% in the presence of 10 mM sodium succinate or sodium citrate (PubMed:35583703).
Activity does not decrease in the presence of 1 mM or 10 mM L-lysine, which has a similar structure to arginine (PubMed:35583703).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.182 mM | argininosuccinate | 8.0 | 45 |
kcat is 6.78 sec-1 with argininosuccinate as substrate.
pH Dependence
Optimum pH is 8.0 (PubMed:35583703).
Shows more than 35% enzymatic activity in terms of relative activity at pH 7.5-9.5 (PubMed:35583703).
Shows more than 35% enzymatic activity in terms of relative activity at pH 7.5-9.5 (PubMed:35583703).
Temperature Dependence
Optimum temperature is 45 degrees Celsius (PubMed:35583703).
Shows more than 35% enzymatic activity in terms of relative activity at 20-50 degrees Celsius (PubMed:35583703).
Inactive above 55 degrees Celsius (PubMed:35583703).
Shows more than 35% enzymatic activity in terms of relative activity at 20-50 degrees Celsius (PubMed:35583703).
Inactive above 55 degrees Celsius (PubMed:35583703).
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | argininosuccinate lyase activity | |
Biological Process | arginine biosynthetic process via ornithine |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameArgininosuccinate lyase
- EC number
- Short namesASAL
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Synechococcales > Merismopediaceae > Synechocystis
Accessions
- Primary accessionP73257
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000137841 | 1-461 | Argininosuccinate lyase | |||
Sequence: MTKKTWSDRFEGTLHPAIALFNASIGFDIELIEYDLDGSIAHGKMLAKTGIISPGEAEQLVQGLEQIRQEYRAGNFNPGVDQEDVHFAVERRLTELVGDVGKKLHTARSRNDQVGTDVRLYLRAQIDDIRQRLRDFQAVLLQLAETNVETLIPGYTHLQRAQPVSLAHHLLAYFQMAQRDWQRLGEIRARTNVSPLGSGALAGTTFPIDRHYSAELLGFAGVYANSLDGVSDRDFAIEFLNAASLIMVHLSRLSEEMILWASQEFSFISLTDSCATGSSIMPQKKNPDVPELIRGKAGRVMGHLQGMLVLMKGLPLAYNKDLQEDKEALFDAVKTVQVSLEAMTILLDEGIVFRQERLAEAVAEDFSNATDVADYLAAKGVPFREAYNLVGKVVKTSLAAGKLLKDLTLAEWQALHPAFEEDIYQAITPQQVVAARNSYGGTGFEQVKMAIANAKAELSQT |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length461
- Mass (Da)50,948
- Last updated1997-02-01 v1
- ChecksumB6E8C81B1CFB5FE2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000022 EMBL· GenBank· DDBJ | BAA17284.1 EMBL· GenBank· DDBJ | Genomic DNA |