P72074 · LST_NEIGO
- ProteinN-acetyllactosaminide alpha-2,3-sialyltransferase
- Genelst
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids371 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the transfer of sialic acid from the substrate CMP-N-acetylneuraminate to the terminal galactose residue of the lacto-N-neotetraose branch of surface lipooligosaccharide (LOS), forming an alpha-2,3-sialyl linkage. Thus, functions in the sialylation of LOS, which plays a role in the evasion of the host immune response by protecting N.gonorrhoeae from complement-mediated serum killing and from phagocytic killing by neutrophils, and which is critical for establishing infection.
Catalytic activity
- a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + CMP + H+
Pathway
Bacterial outer membrane biogenesis; lipooligosaccharide biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 255 | CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Active site | 258 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 278-282 | CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI) | ||||
Sequence: APHPR | ||||||
Active site | 280 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 299-300 | CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI) | ||||
Sequence: IE | ||||||
Binding site | 322-323 | CMP-N-acetyl-beta-neuraminate (UniProtKB | ChEBI) | ||||
Sequence: SG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell outer membrane | |
Molecular Function | glycosyltransferase activity | |
Biological Process | lipopolysaccharide biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameN-acetyllactosaminide alpha-2,3-sialyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Neisseriaceae > Neisseria
Accessions
- Primary accessionP72074
Subcellular Location
UniProt Annotation
GO Annotation
Cell outer membrane ; Peripheral membrane protein
Note: Is exclusively found in outer membrane preparations of the pathogen and is surface exposed. Inner membrane, periplasmic, cytoplasmic, and culture supernatant fractions are devoid of Lst.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene do not express sialyltransferase activity onto LOS.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000080572 | 1-371 | N-acetyllactosaminide alpha-2,3-sialyltransferase | |||
Sequence: MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFNEEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAERAYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEIKTFDDGTNNLIRESSYLGGEFAVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGLKNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQYVAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNVHVYALKPASLPEDYWLKPVYALFRQADIPILTFDDKN |
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length371
- Mass (Da)42,682
- Last updated1997-02-01 v1
- ChecksumC197AD342E87F461