P72010 · G1PDH_METTH
- ProteinGlycerol-1-phosphate dehydrogenase [NAD(P)+]
- GeneegsA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids347 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea. Is also able to catalyze the reverse reaction, i.e. the NAD(P)+-dependent oxidation of G1P but not of G3P. Is not active toward glycerol, dihydroxyacetone, glyceraldehyde-3-phosphate, glyceraldehyde and glycerol-2-phosphate.
Miscellaneous
G1PDH is a pro-R type dehydrogenase, which selectively transfers the pro-R hydrogen from NADH to dihydroxyacetonephosphate.
Catalytic activity
- NAD+ + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H+ + NADH
Cofactor
Note: Binds 1 zinc ion per subunit.
Activity regulation
Partially inhibited by divalent metal cations such as Co2+, Cu2+ and Ni2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.17 mM | DHAP | in the presence of NADH as coenzyme | ||||
0.58 mM | DHAP | in the presence of NADPH as coenzyme | ||||
0.129 mM | NADH | |||||
0.025 mM | NADPH | |||||
16.3 mM | G1P | in the presence of NAD as coenzyme | ||||
4.8 mM | G1P | in the presence of NADP as coenzyme | ||||
0.127 mM | NAD+ | |||||
0.27 mM | NADP+ |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
610 μmol/min/mg | for DHAP reduction with NADH as co | ||||
303 μmol/min/mg | for DHAP reduction with NADPH as co | ||||
39.8 μmol/min/mg | for G1P oxidation with NADH as co | ||||
23.9 μmol/min/mg | for G1P oxidation with NADPH as co |
pH Dependence
Optimum pH is 6.6-7-4. Activity decreases gradually at pH over 7.4 or below 6.6.
Temperature Dependence
Optimum temperature is 75 degrees Celsius.
Pathway
Membrane lipid metabolism; glycerophospholipid metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 94-98 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GKVID | ||||||
Binding site | 116-119 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: TAAS | ||||||
Binding site | 121 | substrate | ||||
Sequence: D | ||||||
Binding site | 125 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 168 | substrate | ||||
Sequence: D | ||||||
Binding site | 168 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 248 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 252 | substrate | ||||
Sequence: H | ||||||
Binding site | 264 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | glycerol-1-phosphate dehydrogenase (NAD+) activity | |
Molecular Function | glycerol-1-phosphate dehydrogenase (NADP+) activity | |
Molecular Function | metal ion binding | |
Biological Process | glycerophospholipid metabolic process | |
Biological Process | phospholipid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlycerol-1-phosphate dehydrogenase [NAD(P)+]
- EC number
- Short namesG1P dehydrogenase; G1PDH
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionP72010
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000157345 | 1-347 | Glycerol-1-phosphate dehydrogenase [NAD(P)+] | |||
Sequence: MDPRKIQLPREIYTGPGVIEDTGRICRDLRFEGRAMVVTGPRTLQIAGEAAIESLQAEGFEVDQVTVDDATMASVRNVQDGLDGVSVVLGVGGGKVIDVAKMSATLEGLHFISVPTAASHDGIASPRASIRNGEGTASLEASSPIGVIADTEIISRAPFRLLASGCADIISNYTAIMDWKLAHRLLNERYSESAAALSLMTAKMIIKSADAIKEGLEESARLAVKSLISSGIAISIAGSSRPASGSEHKFSHALDMIAPKPALHGEQCGVGTIMMMHLHGGDWQFIRDALARINAPTTAAELGIDPEYIIEALTMAHNIRRERYTILGDRGLTREAAERLAKITEVI |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length347
- Mass (Da)36,963
- Last updated1997-02-01 v1
- Checksum46D3600C30515413
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D88555 EMBL· GenBank· DDBJ | BAA13644.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE000666 EMBL· GenBank· DDBJ | AAB85116.1 EMBL· GenBank· DDBJ | Genomic DNA |