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P71875 · KSHA_MYCTU

Function

function

Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,510-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) as substrates.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster per subunit.
Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Binds 1 Fe cation.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3 μM4-BNC722
6.8 μM4-BNC-CoA722
17 μM1,4-BNC-CoA722
24 μMAD725
70 μM1,4-BNC722
110 μMADD725
kcat is 2.7 sec-1 for 1,4-BNC-CoA as substrate (at pH 7 and at 22 degrees Celsius) (PubMed:21987574).
kcat is 0.8 sec-1 for ADD as substrate (at pH 7 and at 25 degrees Celsius) (PubMed:19234303).
kcat is 0.61 sec-1 for 4-BNC-CoA as substrate (at pH 7 and at 22 degrees Celsius) (PubMed:21987574).
kcat is 0.25 sec-1 for 1,4-BNC as substrate (at pH 7 and at 22 degrees Celsius) (PubMed:21987574).
kcat is 0.08 sec-1 for 4-BNC as substrate (at pH 7 and at 22 degrees Celsius) (PubMed:21987574).
kcat is 0.07 sec-1 for AD as substrate (at pH 7 and at 25 degrees Celsius) (PubMed:19234303).

Pathway

Lipid metabolism; steroid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site67[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site69[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site86[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site89[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site175Fe cation (UniProtKB | ChEBI)
Binding site181Fe cation (UniProtKB | ChEBI)
Binding site186Fe cation (UniProtKB | ChEBI)
Binding site304Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function3-ketosteroid 9-alpha-monooxygenase activity
Molecular Functioniron ion binding
Molecular Functionketosteroid monooxygenase activity
Molecular Functionoxidoreductase activity
Biological Processcholesterol catabolic process
Biological Processprotein homotrimerization
Biological Processresponse to cholesterol
Biological Processsteroid biosynthetic process

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    3-ketosteroid-9-alpha-monooxygenase, oxygenase component
  • Alternative names
    • 3-ketosteroid-9-alpha-hydroxylase, oxygenase component
      (KSH
      )
    • Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase
      (EC:1.14.15.30
      ) . EC:1.14.15.30 (UniProtKB | ENZYME | Rhea)
    • Rieske-type oxygenase
      (RO
      )

Gene names

    • Name
      kshA
    • Ordered locus names
      Rv3526

Organism names

Accessions

  • Primary accession
    P71875
  • Secondary accessions
    • L0TCS9

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004040981-3863-ketosteroid-9-alpha-monooxygenase, oxygenase component

Proteomic databases

Expression

Induction

Induced by KstR.

Interaction

Subunit

Homotrimer (PubMed:19234303).
The two-component system 3-ketosteroid-9-alpha-monooxygenase is composed of an oxygenase component KshA and a reductase component KshB (PubMed:21987574).

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain26-128Rieske

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    386
  • Mass (Da)
    44,268
  • Last updated
    1997-07-01 v2
  • MD5 Checksum
    10002F3D8860021E0D543571049511FD
MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMINWLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP46348.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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