P70500 · CDIPT_RAT

  • Protein
    CDP-diacylglycerol--inositol 3-phosphatidyltransferase
  • Gene
    Cdipt
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Catalytic activity is higher with Mg2+ (By similarity).
According to PubMed:7998949 the cofactor is Mn2+, while Mg2+ is much less effective.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site47Mg2+ 1 (UniProtKB | ChEBI)
Binding site47Mg2+ 2 (UniProtKB | ChEBI)
Binding site50Mg2+ 1 (UniProtKB | ChEBI)
Binding site51a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI)
Binding site55a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI)
Binding site61a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI)
Binding site68Mg2+ 1 (UniProtKB | ChEBI)
Binding site68Mg2+ 2 (UniProtKB | ChEBI)
Active site72Proton acceptor
Binding site72Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular ComponentGolgi apparatus
Cellular Componentmembrane
Cellular Componentplasma membrane
Molecular Functionalcohol binding
Molecular Functioncarbohydrate binding
Molecular FunctionCDP-diacylglycerol-inositol 3-phosphatidyltransferase activity
Molecular Functiondiacylglycerol binding
Molecular Functionmanganese ion binding
Biological ProcessCDP-diacylglycerol metabolic process
Biological Processphosphatidylinositol biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CDP-diacylglycerol--inositol 3-phosphatidyltransferase
  • EC number
  • Alternative names
    • Phosphatidylinositol synthase (PI synthase; PtdIns synthase)

Gene names

    • Name
      Cdipt
    • Synonyms
      Pis1

Organism names

  • Taxonomic identifier
  • Strain
    • Wistar
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    P70500

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Multi-pass membrane protein
Cell membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-5Cytoplasmic
Transmembrane6-26Helical
Topological domain27Lumenal
Transmembrane28-48Helical
Topological domain49-73Cytoplasmic
Transmembrane74-94Helical
Topological domain95Lumenal
Transmembrane96-116Helical
Topological domain117-139Cytoplasmic
Transmembrane140-160Helical
Topological domain161-174Lumenal
Transmembrane175-195Helical
Topological domain196-213Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000568041-213CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in liver (at protein level). Widely expressed. Highly expressed in the brain and kidney; lower levels in heart, spleen, lung, liver, skeletal muscle and testis.

Induction

Inhibited by PtdIns (product inhibition).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    213
  • Mass (Da)
    23,613
  • Last updated
    1997-02-01 v1
  • Checksum
    93C7137664EAABB2
MPEENIFLFVPNLIGYARIVFAIISFYFMPCCPFTASSFYLLSGLLDAFDGHAARALNQGTRFGAMLDMLTDRCATMCLLVNLALLYPRATLLFQLSMSLDVASHWLHLHSSVVRGSESHKMIDLSGNPVLRIYYTSRPALFTLCAGNELFYCLLYLFNFSEGPLVGSVGLFRMGLWITAPIALLKSIISVIHLVTAARNMAALDAADRAKKK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8L2Q6A1A0A8L2Q6A1_RATCdipt227

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D82928
EMBL· GenBank· DDBJ
BAA11634.1
EMBL· GenBank· DDBJ
mRNA
AB022890
EMBL· GenBank· DDBJ
BAA82112.1
EMBL· GenBank· DDBJ
Genomic DNA
BC070876
EMBL· GenBank· DDBJ
AAH70876.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp