P70500 · CDIPT_RAT
- ProteinCDP-diacylglycerol--inositol 3-phosphatidyltransferase
- GeneCdipt
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids213 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.
Catalytic activity
- a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H+This reaction proceeds in the forward and the backward directions.
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Catalytic activity is higher with Mg2+ (By similarity).
According to PubMed:7998949 the cofactor is Mn2+, while Mg2+ is much less effective.
According to PubMed:7998949 the cofactor is Mn2+, while Mg2+ is much less effective.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 47 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 47 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 50 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 51 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 55 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 61 | a CDP-1,2-diacyl-sn-glycerol (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 68 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 68 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 72 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 72 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi apparatus | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | alcohol binding | |
Molecular Function | carbohydrate binding | |
Molecular Function | CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity | |
Molecular Function | diacylglycerol binding | |
Molecular Function | manganese ion binding | |
Biological Process | CDP-diacylglycerol metabolic process | |
Biological Process | phosphatidylinositol biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCDP-diacylglycerol--inositol 3-phosphatidyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP70500
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-5 | Cytoplasmic | ||||
Sequence: MPEEN | ||||||
Transmembrane | 6-26 | Helical | ||||
Sequence: IFLFVPNLIGYARIVFAIISF | ||||||
Topological domain | 27 | Lumenal | ||||
Sequence: Y | ||||||
Transmembrane | 28-48 | Helical | ||||
Sequence: FMPCCPFTASSFYLLSGLLDA | ||||||
Topological domain | 49-73 | Cytoplasmic | ||||
Sequence: FDGHAARALNQGTRFGAMLDMLTDR | ||||||
Transmembrane | 74-94 | Helical | ||||
Sequence: CATMCLLVNLALLYPRATLLF | ||||||
Topological domain | 95 | Lumenal | ||||
Sequence: Q | ||||||
Transmembrane | 96-116 | Helical | ||||
Sequence: LSMSLDVASHWLHLHSSVVRG | ||||||
Topological domain | 117-139 | Cytoplasmic | ||||
Sequence: SESHKMIDLSGNPVLRIYYTSRP | ||||||
Transmembrane | 140-160 | Helical | ||||
Sequence: ALFTLCAGNELFYCLLYLFNF | ||||||
Topological domain | 161-174 | Lumenal | ||||
Sequence: SEGPLVGSVGLFRM | ||||||
Transmembrane | 175-195 | Helical | ||||
Sequence: GLWITAPIALLKSIISVIHLV | ||||||
Topological domain | 196-213 | Cytoplasmic | ||||
Sequence: TAARNMAALDAADRAKKK |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000056804 | 1-213 | CDP-diacylglycerol--inositol 3-phosphatidyltransferase | |||
Sequence: MPEENIFLFVPNLIGYARIVFAIISFYFMPCCPFTASSFYLLSGLLDAFDGHAARALNQGTRFGAMLDMLTDRCATMCLLVNLALLYPRATLLFQLSMSLDVASHWLHLHSSVVRGSESHKMIDLSGNPVLRIYYTSRPALFTLCAGNELFYCLLYLFNFSEGPLVGSVGLFRMGLWITAPIALLKSIISVIHLVTAARNMAALDAADRAKKK |
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in liver (at protein level). Widely expressed. Highly expressed in the brain and kidney; lower levels in heart, spleen, lung, liver, skeletal muscle and testis.
Induction
Inhibited by PtdIns (product inhibition).
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length213
- Mass (Da)23,613
- Last updated1997-02-01 v1
- Checksum93C7137664EAABB2
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8L2Q6A1 | A0A8L2Q6A1_RAT | Cdipt | 227 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D82928 EMBL· GenBank· DDBJ | BAA11634.1 EMBL· GenBank· DDBJ | mRNA | ||
AB022890 EMBL· GenBank· DDBJ | BAA82112.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC070876 EMBL· GenBank· DDBJ | AAH70876.1 EMBL· GenBank· DDBJ | mRNA |