P70425 · RIT2_MOUSE
- ProteinGTP-binding protein Rit2
- GeneRit2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids217 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Binds and exchanges GTP and GDP. Binds and modulates the activation of POU4F1 as gene expression regulator.
Miscellaneous
Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Activity regulation
Alternates between an inactive form bound to GDP and an active form bound to GTP.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTP-binding protein Rit2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP70425
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with PLXNB3 at the plasma membrane.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 34 | Dominant negative. Loss of interaction with AFDN, RLF and RALGDS. | ||||
Sequence: S → N | ||||||
Mutagenesis | 52 | Loss of interaction with AFDN, RLF and RALGDS; when associated with L-78. | ||||
Sequence: T → A | ||||||
Mutagenesis | 78 | Constitutively active. Dramatic reduction of the rate of GTP hydrolysis. | ||||
Sequence: Q → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000082728 | 1-217 | GTP-binding protein Rit2 | |||
Sequence: MEVENEAHCCPGSSSGGSREYKVVMLGAGGVGKSAVTMQFISHQFPDYHDPTIEDAYKTQVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIPLVLVGNKIDLEQFRQVSTEEGMNLARDYNCAFFETSAALRFGIDDAFQGLVREIRRKESMLSLVERKLKRKDSLWKKIKASLKKKRENML |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in ganglion cell layer (GCL), inner plexiform layer (IPL) and inner nuclear layer (INL) of the retina. Expressed in retinal ganglion cells (RGCs). Expressed in horizontal, bipolar and amacrine cells, but not Mueller glia, of the INL (at protein level). Neuron-specific (PubMed:8824319).
Expressed in ganglion cell layer (GCL) and inner plexiform layer (IPL) (PubMed:23805044).
Expressed in ganglion cell layer (GCL) and inner plexiform layer (IPL) (PubMed:23805044).
Developmental stage
Expressed weakly in ganglion cell layer (GCL) and inner neuroblastic layer (NBL) of the embryonic retina at 15.5 dpc. Expression increases progressively in the retina from new borns at postnatal day 2 (P2), P5, P15 to 8 week-old adult (at protein level).
Gene expression databases
Interaction
Subunit
Interacts with PLXNB3 (By similarity).
Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Binds calmodulin. Interacts with POU4F1 (via N-terminus); the interaction controls POU4F1 transactivation activity on some neuronal target genes (PubMed:12934100).
Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Binds calmodulin. Interacts with POU4F1 (via N-terminus); the interaction controls POU4F1 transactivation activity on some neuronal target genes (PubMed:12934100).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | P70425 | RLF Q13129 | 2 | EBI-2649620, EBI-958266 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length217
- Mass (Da)24,802
- Last updated1997-02-01 v1
- Checksum189AA6DCCE75034F
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 3 | in Ref. 2; AAD13022 | ||||
Sequence: V → A | ||||||
Sequence conflict | 150 | in Ref. 2; AAD13022 | ||||
Sequence: N → T | ||||||
Sequence conflict | 176 | in Ref. 3; BAC32750 | ||||
Sequence: G → S | ||||||
Sequence conflict | 217 | in Ref. 2; AAD13022 | ||||
Sequence: L → I |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U71202 EMBL· GenBank· DDBJ | AAB42212.1 EMBL· GenBank· DDBJ | mRNA | ||
AF084463 EMBL· GenBank· DDBJ | AAD13022.1 EMBL· GenBank· DDBJ | mRNA | ||
AK040743 EMBL· GenBank· DDBJ | BAC30690.1 EMBL· GenBank· DDBJ | mRNA | ||
AK046425 EMBL· GenBank· DDBJ | BAC32724.1 EMBL· GenBank· DDBJ | mRNA | ||
AK046484 EMBL· GenBank· DDBJ | BAC32750.1 EMBL· GenBank· DDBJ | mRNA | ||
AK138206 EMBL· GenBank· DDBJ | BAE23581.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK139394 EMBL· GenBank· DDBJ | BAE23992.1 EMBL· GenBank· DDBJ | mRNA | ||
AK140133 EMBL· GenBank· DDBJ | BAE24250.1 EMBL· GenBank· DDBJ | mRNA | ||
AK140726 EMBL· GenBank· DDBJ | BAE24457.1 EMBL· GenBank· DDBJ | mRNA | ||
AK162862 EMBL· GenBank· DDBJ | BAE37088.1 EMBL· GenBank· DDBJ | mRNA | ||
AK162953 EMBL· GenBank· DDBJ | BAE37131.1 EMBL· GenBank· DDBJ | mRNA | ||
BC018267 EMBL· GenBank· DDBJ | AAH18267.1 EMBL· GenBank· DDBJ | mRNA |