P70425 · RIT2_MOUSE

  • Protein
    GTP-binding protein Rit2
  • Gene
    Rit2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Binds and exchanges GTP and GDP. Binds and modulates the activation of POU4F1 as gene expression regulator.

Miscellaneous

Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.

Catalytic activity

Activity regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP.

Features

Showing features for binding site.

121720406080100120140160180200
TypeIDPosition(s)Description
Binding site27-34GTP (UniProtKB | ChEBI)
Binding site74-78GTP (UniProtKB | ChEBI)
Binding site133-136GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcell body
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentdendritic tree
Cellular ComponentGolgi apparatus
Cellular Componentmembrane raft
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular Functioncalmodulin binding
Molecular Functionchromatin binding
Molecular FunctionG protein activity
Molecular FunctionGDP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionsemaphorin receptor binding
Biological Processadenylate cyclase-activating G protein-coupled receptor signaling pathway
Biological Processmaintenance of protein location in cell
Biological Processnegative regulation of neuron projection development
Biological Processpositive regulation of MAPK cascade
Biological Processpositive regulation of neuron projection development
Biological Processpositive regulation of transcription by RNA polymerase II
Biological ProcessRas protein signal transduction
Biological Processregulation of calcium-mediated signaling
Biological Processregulation of Cdc42 protein signal transduction
Biological Processregulation of endocytosis
Biological Processregulation of protein phosphorylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    GTP-binding protein Rit2
  • EC number
  • Alternative names
    • Ras-like protein expressed in neurons
    • Ras-like without CAAX protein 2

Gene names

    • Name
      Rit2
    • Synonyms
      Rin, Roc2

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P70425
  • Secondary accessions
    • Q3UST1
    • Q3UUP4
    • Q8BQT5
    • Q9QWX5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis34Dominant negative. Loss of interaction with AFDN, RLF and RALGDS.
Mutagenesis52Loss of interaction with AFDN, RLF and RALGDS; when associated with L-78.
Mutagenesis78Constitutively active. Dramatic reduction of the rate of GTP hydrolysis.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000827281-217GTP-binding protein Rit2

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in ganglion cell layer (GCL), inner plexiform layer (IPL) and inner nuclear layer (INL) of the retina. Expressed in retinal ganglion cells (RGCs). Expressed in horizontal, bipolar and amacrine cells, but not Mueller glia, of the INL (at protein level). Neuron-specific (PubMed:8824319).
Expressed in ganglion cell layer (GCL) and inner plexiform layer (IPL) (PubMed:23805044).

Developmental stage

Expressed weakly in ganglion cell layer (GCL) and inner neuroblastic layer (NBL) of the embryonic retina at 15.5 dpc. Expression increases progressively in the retina from new borns at postnatal day 2 (P2), P5, P15 to 8 week-old adult (at protein level).

Gene expression databases

Interaction

Subunit

Interacts with PLXNB3 (By similarity).
Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Binds calmodulin. Interacts with POU4F1 (via N-terminus); the interaction controls POU4F1 transactivation activity on some neuronal target genes (PubMed:12934100).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO P70425RLF Q131292EBI-2649620, EBI-958266

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    217
  • Mass (Da)
    24,802
  • Last updated
    1997-02-01 v1
  • Checksum
    189AA6DCCE75034F
MEVENEAHCCPGSSSGGSREYKVVMLGAGGVGKSAVTMQFISHQFPDYHDPTIEDAYKTQVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIPLVLVGNKIDLEQFRQVSTEEGMNLARDYNCAFFETSAALRFGIDDAFQGLVREIRRKESMLSLVERKLKRKDSLWKKIKASLKKKRENML

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F8WGU6F8WGU6_MOUSERit277
F2Z432F2Z432_MOUSERit297

Sequence caution

The sequence BAE23581.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict3in Ref. 2; AAD13022
Sequence conflict150in Ref. 2; AAD13022
Sequence conflict176in Ref. 3; BAC32750
Sequence conflict217in Ref. 2; AAD13022

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U71202
EMBL· GenBank· DDBJ
AAB42212.1
EMBL· GenBank· DDBJ
mRNA
AF084463
EMBL· GenBank· DDBJ
AAD13022.1
EMBL· GenBank· DDBJ
mRNA
AK040743
EMBL· GenBank· DDBJ
BAC30690.1
EMBL· GenBank· DDBJ
mRNA
AK046425
EMBL· GenBank· DDBJ
BAC32724.1
EMBL· GenBank· DDBJ
mRNA
AK046484
EMBL· GenBank· DDBJ
BAC32750.1
EMBL· GenBank· DDBJ
mRNA
AK138206
EMBL· GenBank· DDBJ
BAE23581.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK139394
EMBL· GenBank· DDBJ
BAE23992.1
EMBL· GenBank· DDBJ
mRNA
AK140133
EMBL· GenBank· DDBJ
BAE24250.1
EMBL· GenBank· DDBJ
mRNA
AK140726
EMBL· GenBank· DDBJ
BAE24457.1
EMBL· GenBank· DDBJ
mRNA
AK162862
EMBL· GenBank· DDBJ
BAE37088.1
EMBL· GenBank· DDBJ
mRNA
AK162953
EMBL· GenBank· DDBJ
BAE37131.1
EMBL· GenBank· DDBJ
mRNA
BC018267
EMBL· GenBank· DDBJ
AAH18267.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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