P70385 · DHB3_MOUSE

  • Protein
    17-beta-hydroxysteroid dehydrogenase type 3
  • Gene
    Hsd17b3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids (PubMed:32557858).
Favors the reduction of androstenedione to testosterone (PubMed:32557858).
Testosterone is the key androgen driving male development and function (PubMed:32190925, PubMed:32557858).
Uses NADPH while the two other EDH17B enzymes use NADH (By similarity).
Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17. Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (By similarity).
Plays a role in the rate-limiting-step for the maximum level of testosterone production by the testis but does not affect basal testosterone production (PubMed:32190925, PubMed:32557858).

Catalytic activity

  • a 17beta-hydroxy steroid + NADP+ = a 17-oxo steroid + H+ + NADPH
    This reaction proceeds in the backward direction.
  • NADP+ + testosterone = androst-4-ene-3,17-dione + H+ + NADPH
    This reaction proceeds in the backward direction.
    EC:1.1.1.64 (UniProtKB | ENZYME | Rhea)
  • 17beta-estradiol + NADP+ = estrone + H+ + NADPH
    This reaction proceeds in the backward direction.
    EC:1.1.1.62 (UniProtKB | ENZYME | Rhea)
  • 3beta-hydroxyandrost-5-en-17-one + H+ + NADPH = androst-5-en-3beta,17beta-diol + NADP+
    This reaction proceeds in the forward direction.
  • 17beta-hydroxy-5alpha-androstan-3-one + NADP+ = 5alpha-androstan-3,17-dione + H+ + NADPH
    This reaction proceeds in the backward direction.
  • androsterone + H+ + NADPH = 5alpha-androstane-3alpha,17beta-diol + NADP+
    This reaction proceeds in the forward direction.
  • 3beta-hydroxy-5alpha-androstan-17-one + H+ + NADPH = 5alpha-androstane-3beta,17beta-diol + NADP+
    This reaction proceeds in the forward direction.
  • androst-4-ene-3,11,17-trione + H+ + NADPH = 17beta-hydroxyandrost-4-ene-3,11-dione + NADP+
    This reaction proceeds in the forward direction.
  • 11beta-hydroxyandrost-4-ene-3,17-dione + H+ + NADPH = 11beta,17beta-dihydroxyandrost-4-ene-3-one + NADP+
    This reaction proceeds in the forward direction.

Pathway

Hormone biosynthesis; testosterone biosynthesis.
Steroid metabolism.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site44-73NADP+ (UniProtKB | ChEBI)
Binding site181substrate
Active site194Proton acceptor

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Molecular Function17-beta-hydroxysteroid dehydrogenase (NADP+) activity
Molecular Functionestradiol 17-beta-dehydrogenase [NAD(P)+] activity
Molecular Functiontestosterone 17-beta-dehydrogenase (NADP+) activity
Molecular Functiontestosterone dehydrogenase (NAD+) activity
Biological Processsteroid biosynthetic process
Biological Processtestosterone biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    17-beta-hydroxysteroid dehydrogenase type 3
  • Short names
    17-beta-HSD 3
  • Alternative names
    • Estradiol 17-beta-dehydrogenase 2 (EC:1.1.1.62
      ) . EC:1.1.1.62 (UniProtKB | ENZYME | Rhea)
    • Testicular 17-beta-hydroxysteroid dehydrogenase
    • Testosterone 17-beta-dehydrogenase 3
      (EC:1.1.1.64
      ) . EC:1.1.1.64 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      Hsd17b3
    • Synonyms
      Edh17b3

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • OF1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P70385
  • Secondary accessions
    • G3UWF9

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Null males have increased circulating luteinizing hormone (LH) levels (PubMed:32557858).
Null males present mild hypogonadism at adulthood represented by lowered weight of testes and several other androgen-sensitive tissues, a shortened anogenital distance, delayed puberty, and subfertility (fewer litters) (PubMed:32190925).
No phenotypic alterations in the null female (PubMed:32190925, PubMed:32557858).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000545741-30517-beta-hydroxysteroid dehydrogenase type 3

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the testes.

Developmental stage

Expression is restricted to Sertoli cells in fetal life, peaks in neonatal mice, declines thereafter until the age of 21 days, and appears in Leydig cells in adulthood.

Gene expression databases

Interaction

Protein-protein interaction databases

Chemistry

Miscellaneous

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    305
  • Mass (Da)
    34,330
  • Last updated
    2012-10-03 v2
  • Checksum
    8EA9966B2777064D
MEKLFIAAGLFVGLVCLVKCMRFSQHLFLRFCKALPSSFLRSMGQWAVITGAGDGIGKAYSFELARHGLNVVLISRTLEKLQTIAEEIERTTGSCVKIVQADFTREDIYDHIKEHLEGLEIGILVNNVGMLPSFFPSHFLSTSGESQNLIHCNITSVVKMTQLVLKHMESRRKGLILNISSGAALRPWPLYSLYSASKAFVYTFSKALSVEYRDKGIIIQVLTPYSISTPMTKYLNNKMTKTADEFVKESLKYVTIGAESCGCLAHEIIAIILNRIPSRIFYSSTAQRFLLTRYSDYLKRNISNR

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1Y7VJL6A0A1Y7VJL6_MOUSEHsd17b3123
A0A1Y7VJ36A0A1Y7VJ36_MOUSEHsd17b3193

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict95in Ref. 1; AAB06793
Sequence conflict121in Ref. 1; AAB06793
Sequence conflict142in Ref. 1; AAB06793

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U66827
EMBL· GenBank· DDBJ
AAB06793.1
EMBL· GenBank· DDBJ
mRNA
CT009717
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH466631
EMBL· GenBank· DDBJ
EDL16224.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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