P70385 · DHB3_MOUSE
- Protein17-beta-hydroxysteroid dehydrogenase type 3
- GeneHsd17b3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids305 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids (PubMed:32557858).
Favors the reduction of androstenedione to testosterone (PubMed:32557858).
Testosterone is the key androgen driving male development and function (PubMed:32190925, PubMed:32557858).
Uses NADPH while the two other EDH17B enzymes use NADH (By similarity).
Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17. Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (By similarity).
Plays a role in the rate-limiting-step for the maximum level of testosterone production by the testis but does not affect basal testosterone production (PubMed:32190925, PubMed:32557858).
Favors the reduction of androstenedione to testosterone (PubMed:32557858).
Testosterone is the key androgen driving male development and function (PubMed:32190925, PubMed:32557858).
Uses NADPH while the two other EDH17B enzymes use NADH (By similarity).
Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17. Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (By similarity).
Plays a role in the rate-limiting-step for the maximum level of testosterone production by the testis but does not affect basal testosterone production (PubMed:32190925, PubMed:32557858).
Catalytic activity
- a 17beta-hydroxy steroid + NADP+ = a 17-oxo steroid + H+ + NADPHThis reaction proceeds in the backward direction.
- 3beta-hydroxyandrost-5-en-17-one + H+ + NADPH = androst-5-en-3beta,17beta-diol + NADP+This reaction proceeds in the forward direction.
- 17beta-hydroxy-5alpha-androstan-3-one + NADP+ = 5alpha-androstan-3,17-dione + H+ + NADPHThis reaction proceeds in the backward direction.
- androsterone + H+ + NADPH = 5alpha-androstane-3alpha,17beta-diol + NADP+This reaction proceeds in the forward direction.
- 3beta-hydroxy-5alpha-androstan-17-one + H+ + NADPH = 5alpha-androstane-3beta,17beta-diol + NADP+This reaction proceeds in the forward direction.
- androst-4-ene-3,11,17-trione + H+ + NADPH = 17beta-hydroxyandrost-4-ene-3,11-dione + NADP+This reaction proceeds in the forward direction.
- 11beta-hydroxyandrost-4-ene-3,17-dione + H+ + NADPH = 11beta,17beta-dihydroxyandrost-4-ene-3-one + NADP+This reaction proceeds in the forward direction.
Pathway
Hormone biosynthesis; testosterone biosynthesis.
Steroid metabolism.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Molecular Function | 17-beta-hydroxysteroid dehydrogenase (NADP+) activity | |
Molecular Function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity | |
Molecular Function | testosterone 17-beta-dehydrogenase (NADP+) activity | |
Molecular Function | testosterone dehydrogenase (NAD+) activity | |
Biological Process | steroid biosynthetic process | |
Biological Process | testosterone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name17-beta-hydroxysteroid dehydrogenase type 3
- Short names17-beta-HSD 3
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP70385
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Null males have increased circulating luteinizing hormone (LH) levels (PubMed:32557858).
Null males present mild hypogonadism at adulthood represented by lowered weight of testes and several other androgen-sensitive tissues, a shortened anogenital distance, delayed puberty, and subfertility (fewer litters) (PubMed:32190925).
No phenotypic alterations in the null female (PubMed:32190925, PubMed:32557858).
Null males present mild hypogonadism at adulthood represented by lowered weight of testes and several other androgen-sensitive tissues, a shortened anogenital distance, delayed puberty, and subfertility (fewer litters) (PubMed:32190925).
No phenotypic alterations in the null female (PubMed:32190925, PubMed:32557858).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000054574 | 1-305 | 17-beta-hydroxysteroid dehydrogenase type 3 | |||
Sequence: MEKLFIAAGLFVGLVCLVKCMRFSQHLFLRFCKALPSSFLRSMGQWAVITGAGDGIGKAYSFELARHGLNVVLISRTLEKLQTIAEEIERTTGSCVKIVQADFTREDIYDHIKEHLEGLEIGILVNNVGMLPSFFPSHFLSTSGESQNLIHCNITSVVKMTQLVLKHMESRRKGLILNISSGAALRPWPLYSLYSASKAFVYTFSKALSVEYRDKGIIIQVLTPYSISTPMTKYLNNKMTKTADEFVKESLKYVTIGAESCGCLAHEIIAIILNRIPSRIFYSSTAQRFLLTRYSDYLKRNISNR |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the testes.
Developmental stage
Expression is restricted to Sertoli cells in fetal life, peaks in neonatal mice, declines thereafter until the age of 21 days, and appears in Leydig cells in adulthood.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length305
- Mass (Da)34,330
- Last updated2012-10-03 v2
- Checksum8EA9966B2777064D
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1Y7VJL6 | A0A1Y7VJL6_MOUSE | Hsd17b3 | 123 | ||
A0A1Y7VJ36 | A0A1Y7VJ36_MOUSE | Hsd17b3 | 193 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 95 | in Ref. 1; AAB06793 | ||||
Sequence: C → G | ||||||
Sequence conflict | 121 | in Ref. 1; AAB06793 | ||||
Sequence: I → N | ||||||
Sequence conflict | 142 | in Ref. 1; AAB06793 | ||||
Sequence: T → S |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U66827 EMBL· GenBank· DDBJ | AAB06793.1 EMBL· GenBank· DDBJ | mRNA | ||
CT009717 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466631 EMBL· GenBank· DDBJ | EDL16224.1 EMBL· GenBank· DDBJ | Genomic DNA |