P70351 · EZH1_MOUSE
- ProteinHistone-lysine N-methyltransferase EZH1
- GeneEzh1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids747 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Catalytic activity
- L-lysyl27-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl27-[histone H3] + 3 S-adenosyl-L-homocysteine
RHEA-COMP:15548 CHEBI:29969 Position: 27+ 3 CHEBI:59789 = 3 CHEBI:15378 + RHEA-COMP:15535 CHEBI:61961 Position: 27+ 3 CHEBI:57856
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | ESC/E(Z) complex | |
Cellular Component | heterochromatin | |
Cellular Component | nucleus | |
Molecular Function | chromatin binding | |
Molecular Function | histone H3K27 methyltransferase activity | |
Molecular Function | histone H3K27 trimethyltransferase activity | |
Molecular Function | transcription corepressor activity | |
Biological Process | chromatin remodeling | |
Biological Process | facultative heterochromatin formation | |
Biological Process | hepatocyte homeostasis | |
Biological Process | heterochromatin formation | |
Biological Process | liver regeneration | |
Biological Process | methylation | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | response to tetrachloromethane | |
Biological Process | subtelomeric heterochromatin formation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistone-lysine N-methyltransferase EZH1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP70351
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with trimethylated 'Lys-27' of histone H3.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000213991 | 1-747 | Histone-lysine N-methyltransferase EZH1 | |||
Sequence: MDIASPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQPVQPMKPVSGHPFLKKCTIESIFPGFDSQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEDGHNDPSDGKQDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEYAMLHNPRSKCSGRRRRRHPVVSASCSNASASAMAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASPAPAQLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPAQKKKRKHRLWAAHCRKIQLKKDNNSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF | ||||||
Cross-link | 327 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at high levels in kidney, adrenal gland, testis and brain.
Gene expression databases
Interaction
Subunit
Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM). Interacts with EZHIP; the interaction blocks EZH1 methyltransferase activity (PubMed:31451685).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P70351 | Jarid2 Q62315 | 4 | EBI-2531737, EBI-493592 | |
BINARY | P70351 | Mtf2 Q02395 | 3 | EBI-2531737, EBI-2531578 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 188-216 | Basic and acidic residues | ||||
Sequence: DEEEDGHNDPSDGKQDDSKEDLPVTRKRK | ||||||
Region | 188-231 | Disordered | ||||
Sequence: DEEEDGHNDPSDGKQDDSKEDLPVTRKRKRHAIEGNKKSSKKQF | ||||||
Region | 368-414 | Disordered | ||||
Sequence: VSASCSNASASAMAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQK | ||||||
Compositional bias | 393-414 | Polar residues | ||||
Sequence: GNDWASSSSEANSRCQTPTKQK | ||||||
Motif | 491-496 | Nuclear localization signal | ||||
Sequence: QKKKRK | ||||||
Domain | 504-606 | CXC | ||||
Sequence: CRKIQLKKDNNSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCS | ||||||
Domain | 613-728 | SET | ||||
Sequence: KHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYR |
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P70351-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length747
- Mass (Da)85,188
- Last updated1997-02-01 v1
- Checksum43CECFBDF3E49192
P70351-2
- Name2
- Differences from canonical
- 555-747: QNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF → KSTLLSPSSTQVVGLGVPRLFSPAP
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0R4J1C0 | A0A0R4J1C0_MOUSE | Ezh1 | 750 |
Sequence caution
Features
Showing features for sequence conflict, compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 84 | in Ref. 5; BAE32680 | ||||
Sequence: T → A | ||||||
Compositional bias | 188-216 | Basic and acidic residues | ||||
Sequence: DEEEDGHNDPSDGKQDDSKEDLPVTRKRK | ||||||
Compositional bias | 393-414 | Polar residues | ||||
Sequence: GNDWASSSSEANSRCQTPTKQK | ||||||
Sequence conflict | 446 | in Ref. 2; AAD54021 | ||||
Sequence: H → Y | ||||||
Sequence conflict | 452 | in Ref. 2; AAD54021 | ||||
Sequence: N → Y | ||||||
Alternative sequence | VSP_036388 | 555-747 | in isoform 2 | |||
Sequence: QNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF → KSTLLSPSSTQVVGLGVPRLFSPAP |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U60453 EMBL· GenBank· DDBJ | AAC53279.1 EMBL· GenBank· DDBJ | mRNA | ||
AB004817 EMBL· GenBank· DDBJ | BAA25018.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AF104360 EMBL· GenBank· DDBJ | AAD54021.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF483490 EMBL· GenBank· DDBJ | AAL90764.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AF483491 EMBL· GenBank· DDBJ | AAL90765.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK045374 EMBL· GenBank· DDBJ | BAC32334.1 EMBL· GenBank· DDBJ | mRNA | ||
AK138942 EMBL· GenBank· DDBJ | BAE23827.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK140694 EMBL· GenBank· DDBJ | BAE24446.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK154565 EMBL· GenBank· DDBJ | BAE32680.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK164192 EMBL· GenBank· DDBJ | BAE37674.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AL590969 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466677 EMBL· GenBank· DDBJ | EDL03899.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
BC007135 EMBL· GenBank· DDBJ | AAH07135.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |