P70351 · EZH1_MOUSE

  • Protein
    Histone-lysine N-methyltransferase EZH1
  • Gene
    Ezh1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Catalytic activity

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromosome, telomeric region
Cellular ComponentESC/E(Z) complex
Cellular Componentheterochromatin
Cellular Componentnucleus
Molecular Functionchromatin binding
Molecular Functionhistone H3K27 methyltransferase activity
Molecular Functionhistone H3K27 trimethyltransferase activity
Molecular Functiontranscription corepressor activity
Biological Processchromatin remodeling
Biological Processfacultative heterochromatin formation
Biological Processhepatocyte homeostasis
Biological Processheterochromatin formation
Biological Processliver regeneration
Biological Processmethylation
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processresponse to tetrachloromethane
Biological Processsubtelomeric heterochromatin formation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Histone-lysine N-methyltransferase EZH1
  • EC number
  • Alternative names
    • ENX-2
    • Enhancer of zeste homolog 1

Gene names

    • Name
      Ezh1
    • Synonyms
      Enx2

Organism names

  • Taxonomic identifier
  • Strains
    • 129/Sv
    • ILS
    • ISS
    • C57BL/6J
    • NOD
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P70351
  • Secondary accessions
    • A2A4K5
    • Q3TPR1
    • Q3U3V5
    • Q3UU02
    • Q8BR85

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Note: Colocalizes with trimethylated 'Lys-27' of histone H3.

Keywords

PTM/Processing

Features

Showing features for chain, cross-link.

TypeIDPosition(s)Description
ChainPRO_00002139911-747Histone-lysine N-methyltransferase EZH1
Cross-link327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed at high levels in kidney, adrenal gland, testis and brain.

Gene expression databases

Interaction

Subunit

Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM). Interacts with EZHIP; the interaction blocks EZH1 methyltransferase activity (PubMed:31451685).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P70351Jarid2 Q623154EBI-2531737, EBI-493592
BINARY P70351Mtf2 Q023953EBI-2531737, EBI-2531578

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for compositional bias, region, motif, domain.

TypeIDPosition(s)Description
Compositional bias188-216Basic and acidic residues
Region188-231Disordered
Region368-414Disordered
Compositional bias393-414Polar residues
Motif491-496Nuclear localization signal
Domain504-606CXC
Domain613-728SET

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

P70351-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    747
  • Mass (Da)
    85,188
  • Last updated
    1997-02-01 v1
  • Checksum
    43CECFBDF3E49192
MDIASPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQPVQPMKPVSGHPFLKKCTIESIFPGFDSQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEDGHNDPSDGKQDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEYAMLHNPRSKCSGRRRRRHPVVSASCSNASASAMAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASPAPAQLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPAQKKKRKHRLWAAHCRKIQLKKDNNSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF

P70351-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 555-747: QNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF → KSTLLSPSSTQVVGLGVPRLFSPAP

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0R4J1C0A0A0R4J1C0_MOUSEEzh1750

Sequence caution

The sequence AAH07135.1 differs from that shown. Reason: Erroneous initiation
The sequence AAL90764.1 differs from that shown. Reason: Erroneous initiation
The sequence AAL90765.1 differs from that shown. Reason: Erroneous initiation
The sequence BAA25018.1 differs from that shown. Reason: Erroneous initiation
The sequence BAE23827.1 differs from that shown. Reason: Erroneous initiation
The sequence BAE24446.1 differs from that shown. Reason: Erroneous initiation
The sequence BAE32680.1 differs from that shown. Reason: Erroneous initiation
The sequence BAE37674.1 differs from that shown. Reason: Erroneous initiation
The sequence EDL03899.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict, compositional bias, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict84in Ref. 5; BAE32680
Compositional bias188-216Basic and acidic residues
Compositional bias393-414Polar residues
Sequence conflict446in Ref. 2; AAD54021
Sequence conflict452in Ref. 2; AAD54021
Alternative sequenceVSP_036388555-747in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U60453
EMBL· GenBank· DDBJ
AAC53279.1
EMBL· GenBank· DDBJ
mRNA
AB004817
EMBL· GenBank· DDBJ
BAA25018.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AF104360
EMBL· GenBank· DDBJ
AAD54021.1
EMBL· GenBank· DDBJ
Genomic DNA
AF483490
EMBL· GenBank· DDBJ
AAL90764.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AF483491
EMBL· GenBank· DDBJ
AAL90765.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK045374
EMBL· GenBank· DDBJ
BAC32334.1
EMBL· GenBank· DDBJ
mRNA
AK138942
EMBL· GenBank· DDBJ
BAE23827.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK140694
EMBL· GenBank· DDBJ
BAE24446.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK154565
EMBL· GenBank· DDBJ
BAE32680.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK164192
EMBL· GenBank· DDBJ
BAE37674.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AL590969
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH466677
EMBL· GenBank· DDBJ
EDL03899.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
BC007135
EMBL· GenBank· DDBJ
AAH07135.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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