P70318 · TIAR_MOUSE
- ProteinNucleolysin TIAR
- GeneTial1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids392 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation (By similarity).
Shows a preference for uridine-rich RNAs (By similarity).
Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA (PubMed:11514562).
Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1 (PubMed:17488725).
Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG) (By similarity).
Possesses nucleolytic activity against cytotoxic lymphocyte target cells (By similarity).
May be involved in apoptosis (By similarity).
Shows a preference for uridine-rich RNAs (By similarity).
Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA (PubMed:11514562).
Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1 (PubMed:17488725).
Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG) (By similarity).
Possesses nucleolytic activity against cytotoxic lymphocyte target cells (By similarity).
May be involved in apoptosis (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytolytic granule | |
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic stress granule | |
Cellular Component | cytosol | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | ribonucleoprotein complex | |
Molecular Function | DNA binding | |
Molecular Function | mRNA 3'-UTR AU-rich region binding | |
Molecular Function | protein-RNA adaptor activity | |
Biological Process | apoptotic process | |
Biological Process | germ cell development | |
Biological Process | negative regulation of cell population proliferation | |
Biological Process | positive regulation of hippo signaling | |
Biological Process | positive regulation of miRNA-mediated gene silencing | |
Biological Process | positive regulation of stem cell proliferation | |
Biological Process | stem cell division | |
Biological Process | stem cell proliferation |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNucleolysin TIAR
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP70318
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Nuclear import seems to be coupled to RNA polymerase II transcription and may be dependent on RNA-binding (PubMed:16278295).
Accumulates in cytoplasmic stress granules (SG) following cellular damage (By similarity).
Accumulates in cytoplasmic stress granules (SG) following cellular damage (By similarity).
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 117-121 | Impairs nuclear localization. | ||||
Sequence: FVGDL → DVPDD | ||||||
Mutagenesis | 156-160 | Impairs nuclear localization. | ||||
Sequence: GYGFV → PDGDE | ||||||
Mutagenesis | 225-229 | Abolishes nuclear export. | ||||
Sequence: YCGGI → DRPPD | ||||||
Mutagenesis | 258-264 | Abolishes nuclear export. | ||||
Sequence: GYSFVRF → PDSDERD | ||||||
Mutagenesis | 293-300 | No effect on nuclear localization. | ||||
Sequence: KESPDMTK → RESPDMTR |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000081979 | 1-392 | Nucleolysin TIAR | |||
Sequence: MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEQPDSRRVNSSVGFSVLQHTSNDPYCFVEFYEHRDAAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPFGKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQETNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVYGNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASFPTQ | ||||||
Modified residue | 139 | N6-acetyllysine | ||||
Sequence: K | ||||||
Modified residue | 218 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed both in primordial germ cells (PGCs) and in neighboring somatic cells.
Gene expression databases
Interaction
Subunit
Interacts with FASTK.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P70318 | Ddx21 Q8K2L4 | 2 | EBI-299820, EBI-7977861 | |
BINARY | P70318 | Hnrnpm Q3THB3 | 2 | EBI-299820, EBI-4282050 | |
BINARY | P70318 | Srsf1 Q6PDM2 | 3 | EBI-299820, EBI-2550360 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 9-102 | RRM 1 | ||||
Sequence: RTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEQPDSRRVNSSVGFSVLQHTSNDPYCFVEFYEHRDAAAALAAMNGRKILGKEVKVNWATT | ||||||
Domain | 114-192 | RRM 2 | ||||
Sequence: FHVFVGDLSPEITTEDIKSAFAPFGKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATR | ||||||
Domain | 222-294 | RRM 3 | ||||
Sequence: CTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKE | ||||||
Region | 363-392 | Disordered | ||||
Sequence: GAQPPQGQAPPPVIPPPNQAGYGMASFPTQ |
Domain
The RRM 2 domain is required for the binding to target RNA, and the RRM 1 and RRM 3 domains seem to contribute to the affinity of the interaction with RNA.
The RRM2 domain and the C-terminal residues 290-339 contribute to nuclear localization.
The RRM3 domain mediates nuclear export and cytoplasmic localization in a manner dependent on RNA- binding.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length392
- Mass (Da)43,389
- Last updated1997-02-01 v1
- Checksum66E6AF14B9EBE77A
Computationally mapped potential isoform sequences
There are 8 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0U1RPL4 | A0A0U1RPL4_MOUSE | Tial1 | 42 | ||
A0A0U1RPD3 | A0A0U1RPD3_MOUSE | Tial1 | 244 | ||
A0A0U1RPE1 | A0A0U1RPE1_MOUSE | Tial1 | 106 | ||
A0A0U1RPB0 | A0A0U1RPB0_MOUSE | Tial1 | 140 | ||
A0A0U1RP00 | A0A0U1RP00_MOUSE | Tial1 | 107 | ||
D3Z3Y4 | D3Z3Y4_MOUSE | Tial1 | 336 | ||
D6RGU1 | D6RGU1_MOUSE | Tial1 | 135 | ||
Q921W2 | Q921W2_MOUSE | Tial1 | 375 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U55861 EMBL· GenBank· DDBJ | AAC52870.1 EMBL· GenBank· DDBJ | mRNA |