P69791 · PTQA_ECOLI

  • Protein
    PTS system N,N'-diacetylchitobiose-specific EIIA component
  • Gene
    chbA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913117, PubMed:10913118).
Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Can also use copper and nickel with lower efficiency.

Features

Showing features for active site.

1116102030405060708090100110
TypeIDPosition(s)Description
Active site89Tele-phosphohistidine intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentprotein-containing complex
Cellular Componenttransmembrane transporter complex
Molecular Functionprotein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity
Biological ProcessN,N'-diacetylchitobiose import
Biological Processphosphoenolpyruvate-dependent sugar phosphotransferase system

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 4.A.3.2.1the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

Names & Taxonomy

Protein names

  • Recommended name
    PTS system N,N'-diacetylchitobiose-specific EIIA component
  • Alternative names
    • EIIA-Chb
    • EIII-Chb
    • IIIcel
    • N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component

Gene names

    • Name
      chbA
    • Synonyms
      celC
    • Ordered locus names
      b1736, JW1725

Organism names

  • Taxonomic identifier
  • Strains
    • 6
    • 28
    • 35
    • 37
    • 50
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P69791
  • Secondary accessions
    • P17335
    • Q47092
    • Q47093
    • Q47094
    • Q57128

Proteomes

Subcellular Location

Phenotypes & Variants

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant14in strain: ECOR 1
Natural variant52in strain: ECOR 61
Natural variant59in strain: ECOR 50
Mutagenesis92Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001864941-116PTS system N,N'-diacetylchitobiose-specific EIIA component
Modified residue89Phosphohistidine; by HPr

Keywords

Proteomic databases

PTM databases

Expression

Induction

By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).

Interaction

Subunit

Forms a complex with ChbB (EIIB) (PubMed:10913119, PubMed:10913122, PubMed:19959833).
ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574).
The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P69791chbB P697952EBI-1121924, EBI-1128186
View interactors in UniProtKB
View CPX-4683 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain15-113PTS EIIA type-3

Domain

The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    116
  • Mass (Da)
    12,748
  • Last updated
    2005-05-10 v1
  • Checksum
    F2951DC6700FA8A9
MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQGDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARELITELIELHEKLKA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict43-44in Ref. 1; CAA37071

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X52890
EMBL· GenBank· DDBJ
CAA37071.1
EMBL· GenBank· DDBJ
Genomic DNA
M93570
EMBL· GenBank· DDBJ
AAA23551.1
EMBL· GenBank· DDBJ
Genomic DNA
M93571
EMBL· GenBank· DDBJ
AAA23553.1
EMBL· GenBank· DDBJ
Genomic DNA
M93572
EMBL· GenBank· DDBJ
AAA23554.1
EMBL· GenBank· DDBJ
Genomic DNA
M93573
EMBL· GenBank· DDBJ
AAA23559.1
EMBL· GenBank· DDBJ
Genomic DNA
M93574
EMBL· GenBank· DDBJ
AAA23556.1
EMBL· GenBank· DDBJ
Genomic DNA
M93575
EMBL· GenBank· DDBJ
AAA23552.1
EMBL· GenBank· DDBJ
Genomic DNA
M93576
EMBL· GenBank· DDBJ
AAA23560.1
EMBL· GenBank· DDBJ
Genomic DNA
M93577
EMBL· GenBank· DDBJ
AAA23557.1
EMBL· GenBank· DDBJ
Genomic DNA
M93591
EMBL· GenBank· DDBJ
AAA23561.1
EMBL· GenBank· DDBJ
Genomic DNA
M93592
EMBL· GenBank· DDBJ
AAA23555.1
EMBL· GenBank· DDBJ
Genomic DNA
M93593
EMBL· GenBank· DDBJ
AAA23558.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC74806.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA15517.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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