P69791 · PTQA_ECOLI
- ProteinPTS system N,N'-diacetylchitobiose-specific EIIA component
- GenechbA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids116 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913117, PubMed:10913118).
Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).
Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).
Cofactor
Note: Can also use copper and nickel with lower efficiency.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 89 | Tele-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | protein-containing complex | |
Cellular Component | transmembrane transporter complex | |
Molecular Function | protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity | |
Biological Process | N,N'-diacetylchitobiose import | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePTS system N,N'-diacetylchitobiose-specific EIIA component
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP69791
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 14 | in strain: ECOR 1 | ||||
Sequence: A → V | ||||||
Natural variant | 52 | in strain: ECOR 61 | ||||
Sequence: A → T | ||||||
Natural variant | 59 | in strain: ECOR 50 | ||||
Sequence: M → I | ||||||
Mutagenesis | 92 | Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation. | ||||
Sequence: D → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000186494 | 1-116 | PTS system N,N'-diacetylchitobiose-specific EIIA component | |||
Sequence: MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQGDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARELITELIELHEKLKA | ||||||
Modified residue | 89 | Phosphohistidine; by HPr | ||||
Sequence: H |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).
Interaction
Subunit
Forms a complex with ChbB (EIIB) (PubMed:10913119, PubMed:10913122, PubMed:19959833).
ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574).
The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).
ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574).
The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P69791 | chbB P69795 | 2 | EBI-1121924, EBI-1128186 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-113 | PTS EIIA type-3 | ||||
Sequence: EELEEVVMGLIINSGQARSLAYAALKQAKQGDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARELITELIELHEK |
Domain
The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length116
- Mass (Da)12,748
- Last updated2005-05-10 v1
- ChecksumF2951DC6700FA8A9
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 43-44 | in Ref. 1; CAA37071 | ||||
Sequence: KQ → NR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X52890 EMBL· GenBank· DDBJ | CAA37071.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93570 EMBL· GenBank· DDBJ | AAA23551.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93571 EMBL· GenBank· DDBJ | AAA23553.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93572 EMBL· GenBank· DDBJ | AAA23554.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93573 EMBL· GenBank· DDBJ | AAA23559.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93574 EMBL· GenBank· DDBJ | AAA23556.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93575 EMBL· GenBank· DDBJ | AAA23552.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93576 EMBL· GenBank· DDBJ | AAA23560.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93577 EMBL· GenBank· DDBJ | AAA23557.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93591 EMBL· GenBank· DDBJ | AAA23561.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93592 EMBL· GenBank· DDBJ | AAA23555.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M93593 EMBL· GenBank· DDBJ | AAA23558.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC74806.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA15517.1 EMBL· GenBank· DDBJ | Genomic DNA |