P69744 · TRPV5_MOUSE

  • Protein
    Transient receptor potential cation channel subfamily V member 5
  • Gene
    Trpv5
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine (PubMed:12077127, PubMed:14679186).
Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (PubMed:14679186).
The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (PubMed:12077127).
Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).

Catalytic activity

Activity regulation

Activated by WNK3.

Features

Showing features for binding site.

1723100200300400500600700
TypeIDPosition(s)Description
Binding site535Ca2+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentapical plasma membrane
Cellular Componentcalcium channel complex
Cellular Componentplasma membrane
Molecular Functioncalcium channel activity
Molecular Functioncalmodulin binding
Molecular Functionmetal ion binding
Biological Processcalcium ion homeostasis
Biological Processcalcium ion import across plasma membrane
Biological Processcalcium ion transmembrane transport
Biological Processcalcium ion transport
Biological Processcalcium ion transport into cytosol
Biological Processintracellular calcium ion homeostasis
Biological Processprotein homotetramerization
Biological Processregulation of urine volume

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transient receptor potential cation channel subfamily V member 5
  • Short names
    TrpV5
  • Alternative names
    • Calcium transport protein 2 (CaT2)
    • Epithelial calcium channel 1 (ECaC1)
    • Osm-9-like TRP channel 3 (OTRPC3)

Gene names

    • Name
      Trpv5

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P69744
  • Secondary accessions
    • Q2TB50

Proteomes

Organism-specific databases

Subcellular Location

Apical cell membrane
; Multi-pass membrane protein
Note: Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells.

Features

Showing features for topological domain, transmembrane, intramembrane.

TypeIDPosition(s)Description
Topological domain1-320Cytoplasmic
Transmembrane321-341Helical
Topological domain342-378Extracellular
Transmembrane379-401Helical
Topological domain402-412Extracellular
Transmembrane413-435Helical
Topological domain436-441Cytoplasmic
Transmembrane442-462Helical
Topological domain463-485Extracellular
Transmembrane486-506Helical
Intramembrane517-537Pore-forming
Transmembrane550-570Helical
Topological domain571-723Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Mutant mice appear grossly normal and are fertile. They display polyuria and increased urinary excretion of Ca2+, due to defective Ca2+ reabsorption in the kidney distal convoluted tubules. Likewise, they display increased urinary excretion of phosphate. Besides, their urine has a lower pH. The polyuria and the urine acidification may be a response to the high urinary Ca2+ levels, preventing the formation of kidney stones (PubMed:14679186).
Serum Ca2+ and phosphate levels are normal, probably due to increased expression of TRPV6 and increased Ca2+ absorption in the intestine. The increased expression of TRPV6 may be due to the increased serum levels of 1,25-dihydroxy-vitamin D3 that are observed in mutant mice (PubMed:14679186, PubMed:27102152).
Age-related changes in trabecular and cortical bone mass are accelerated in male mutant mice, including reduced trabecular and cortical bone thickness. Still, this has no effect on bone strength (PubMed:27102152).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis403-406Induces faster Ca2+-dependent channel inactivation.
Mutagenesis593Abolishes interaction with S100A10, plasma membrane localization and channel activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002153511-723Transient receptor potential cation channel subfamily V member 5
Modified residue678Phosphothreonine
Modified residue682Phosphoserine

Post-translational modification

Glycosylated.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in kidney cortex (at protein level).

Gene expression databases

Interaction

Subunit

Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6 (PubMed:12574114).
Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane (PubMed:12660155).
Interacts with calmodulin (PubMed:15123711).
Interacts with BSPRY, which results in its inactivation (PubMed:16380433).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P69744Wnk4 Q80UE62EBI-538447, EBI-295378

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for repeat, region, compositional bias.

TypeIDPosition(s)Description
Repeat38-68ANK 1
Repeat72-101ANK 2
Repeat110-139ANK 3
Repeat156-185ANK 4
Repeat189-222ANK 5
Repeat232-261ANK 6
Region591-595Interaction with S100A10
Region643-646Involved in Ca2+-dependent inactivation
Region651-673Disordered
Compositional bias657-673Polar residues
Region693-723Involved in Ca2+-dependent inactivation

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    723
  • Mass (Da)
    82,218
  • Last updated
    2011-07-27 v2
  • Checksum
    6668C67C47CBB443
MGAKTPWIQLQKLLNWWVRDQDWNQHVDQLHMLQQKSIWESPLLRAAKENDMCTLKKLQHDQNCDFRQRGALGETALHVAALYDNLDAAIMLMEAAPYLVTESTLCEPFVGQTALHIAVMNQNVNLVRALLARGASASARATGSAFHRSSHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILVLQPNKTFACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNTVMFQHLMQKRKRIQWSFGPLTSSLYDLTEIDSWGEELSFLELVVSSKKKEARQILEQTPVKELVSLKWKKYGQPYFCLLGALYIFYMVCFTTCCVYRPLKFRDANRTHVRDNTIMEQKSLQEAYVTYQDKIRLVGELVTVIGAVIILLLEIPDIFRVGASRYFGQTVLGGPFHVIIITYASLVLLTMAMRLTNVNGEVVPMSMALVLGWCSVMYFARGFQMLGPFTIMIQKMIFGDLLRFCWLMAMVILGFASAFYIIFQTEDPDNLGEFSDYPTAMFSTFELFLTIIDGPANYRVDLPFMYSVTYATFAIIATLLMLNLFIAMMGDTHWRVAQERDELWRAQVVATTVMLERKMPRFLWPRSGICGCEYGLGDRWFLRVEHHQEQNPYRVLRYVEAFKSSDKEEVQEQLSEKQPSGTETGTLARGSVVLQTPPLSRTTSLSSNSHRGWEILRRNTLGHLNLGLDPGEGDGEEIYQF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0A6YW69A0A0A6YW69_MOUSETrpv5674

Sequence caution

The sequence AK085479 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict302in Ref. 2; AK085479
Compositional bias657-673Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF336378
EMBL· GenBank· DDBJ
AAM53408.1
EMBL· GenBank· DDBJ
Genomic DNA
AK085479
EMBL· GenBank· DDBJ
-mRNA No translation available.
BC110554
EMBL· GenBank· DDBJ
AAI10555.1
EMBL· GenBank· DDBJ
mRNA
BC110555
EMBL· GenBank· DDBJ
AAI10556.1
EMBL· GenBank· DDBJ
mRNA
CH466533
EMBL· GenBank· DDBJ
EDL13503.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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