P69744 · TRPV5_MOUSE
- ProteinTransient receptor potential cation channel subfamily V member 5
- GeneTrpv5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids723 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine (PubMed:12077127, PubMed:14679186).
Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (PubMed:14679186).
The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (PubMed:12077127).
Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (PubMed:14679186).
The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (PubMed:12077127).
Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Catalytic activity
- Ca2+(in) = Ca2+(out)
Activity regulation
Activated by WNK3.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | calcium channel complex | |
Cellular Component | plasma membrane | |
Molecular Function | calcium channel activity | |
Molecular Function | calmodulin binding | |
Molecular Function | metal ion binding | |
Biological Process | calcium ion homeostasis | |
Biological Process | calcium ion import across plasma membrane | |
Biological Process | calcium ion transmembrane transport | |
Biological Process | calcium ion transport | |
Biological Process | calcium ion transport into cytosol | |
Biological Process | intracellular calcium ion homeostasis | |
Biological Process | protein homotetramerization | |
Biological Process | regulation of urine volume |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTransient receptor potential cation channel subfamily V member 5
- Short namesTrpV5
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP69744
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Multi-pass membrane protein
Note: Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells.
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-320 | Cytoplasmic | ||||
Sequence: MGAKTPWIQLQKLLNWWVRDQDWNQHVDQLHMLQQKSIWESPLLRAAKENDMCTLKKLQHDQNCDFRQRGALGETALHVAALYDNLDAAIMLMEAAPYLVTESTLCEPFVGQTALHIAVMNQNVNLVRALLARGASASARATGSAFHRSSHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILVLQPNKTFACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNTVMFQHLMQKRKRIQWSFGPLTSSLYDLTEIDSWGEELSFLELVVSSKKKEARQILEQTPVKELVSLKWKKYGQP | ||||||
Transmembrane | 321-341 | Helical | ||||
Sequence: YFCLLGALYIFYMVCFTTCCV | ||||||
Topological domain | 342-378 | Extracellular | ||||
Sequence: YRPLKFRDANRTHVRDNTIMEQKSLQEAYVTYQDKIR | ||||||
Transmembrane | 379-401 | Helical | ||||
Sequence: LVGELVTVIGAVIILLLEIPDIF | ||||||
Topological domain | 402-412 | Extracellular | ||||
Sequence: RVGASRYFGQT | ||||||
Transmembrane | 413-435 | Helical | ||||
Sequence: VLGGPFHVIIITYASLVLLTMAM | ||||||
Topological domain | 436-441 | Cytoplasmic | ||||
Sequence: RLTNVN | ||||||
Transmembrane | 442-462 | Helical | ||||
Sequence: GEVVPMSMALVLGWCSVMYFA | ||||||
Topological domain | 463-485 | Extracellular | ||||
Sequence: RGFQMLGPFTIMIQKMIFGDLLR | ||||||
Transmembrane | 486-506 | Helical | ||||
Sequence: FCWLMAMVILGFASAFYIIFQ | ||||||
Intramembrane | 517-537 | Pore-forming | ||||
Sequence: SDYPTAMFSTFELFLTIIDGP | ||||||
Transmembrane | 550-570 | Helical | ||||
Sequence: VTYATFAIIATLLMLNLFIAM | ||||||
Topological domain | 571-723 | Cytoplasmic | ||||
Sequence: MGDTHWRVAQERDELWRAQVVATTVMLERKMPRFLWPRSGICGCEYGLGDRWFLRVEHHQEQNPYRVLRYVEAFKSSDKEEVQEQLSEKQPSGTETGTLARGSVVLQTPPLSRTTSLSSNSHRGWEILRRNTLGHLNLGLDPGEGDGEEIYQF |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mutant mice appear grossly normal and are fertile. They display polyuria and increased urinary excretion of Ca2+, due to defective Ca2+ reabsorption in the kidney distal convoluted tubules. Likewise, they display increased urinary excretion of phosphate. Besides, their urine has a lower pH. The polyuria and the urine acidification may be a response to the high urinary Ca2+ levels, preventing the formation of kidney stones (PubMed:14679186).
Serum Ca2+ and phosphate levels are normal, probably due to increased expression of TRPV6 and increased Ca2+ absorption in the intestine. The increased expression of TRPV6 may be due to the increased serum levels of 1,25-dihydroxy-vitamin D3 that are observed in mutant mice (PubMed:14679186, PubMed:27102152).
Age-related changes in trabecular and cortical bone mass are accelerated in male mutant mice, including reduced trabecular and cortical bone thickness. Still, this has no effect on bone strength (PubMed:27102152).
Serum Ca2+ and phosphate levels are normal, probably due to increased expression of TRPV6 and increased Ca2+ absorption in the intestine. The increased expression of TRPV6 may be due to the increased serum levels of 1,25-dihydroxy-vitamin D3 that are observed in mutant mice (PubMed:14679186, PubMed:27102152).
Age-related changes in trabecular and cortical bone mass are accelerated in male mutant mice, including reduced trabecular and cortical bone thickness. Still, this has no effect on bone strength (PubMed:27102152).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 403-406 | Induces faster Ca2+-dependent channel inactivation. | ||||
Sequence: VGAS → LGVT | ||||||
Mutagenesis | 593 | Abolishes interaction with S100A10, plasma membrane localization and channel activity. | ||||
Sequence: T → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000215351 | 1-723 | Transient receptor potential cation channel subfamily V member 5 | |||
Sequence: MGAKTPWIQLQKLLNWWVRDQDWNQHVDQLHMLQQKSIWESPLLRAAKENDMCTLKKLQHDQNCDFRQRGALGETALHVAALYDNLDAAIMLMEAAPYLVTESTLCEPFVGQTALHIAVMNQNVNLVRALLARGASASARATGSAFHRSSHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILVLQPNKTFACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNTVMFQHLMQKRKRIQWSFGPLTSSLYDLTEIDSWGEELSFLELVVSSKKKEARQILEQTPVKELVSLKWKKYGQPYFCLLGALYIFYMVCFTTCCVYRPLKFRDANRTHVRDNTIMEQKSLQEAYVTYQDKIRLVGELVTVIGAVIILLLEIPDIFRVGASRYFGQTVLGGPFHVIIITYASLVLLTMAMRLTNVNGEVVPMSMALVLGWCSVMYFARGFQMLGPFTIMIQKMIFGDLLRFCWLMAMVILGFASAFYIIFQTEDPDNLGEFSDYPTAMFSTFELFLTIIDGPANYRVDLPFMYSVTYATFAIIATLLMLNLFIAMMGDTHWRVAQERDELWRAQVVATTVMLERKMPRFLWPRSGICGCEYGLGDRWFLRVEHHQEQNPYRVLRYVEAFKSSDKEEVQEQLSEKQPSGTETGTLARGSVVLQTPPLSRTTSLSSNSHRGWEILRRNTLGHLNLGLDPGEGDGEEIYQF | ||||||
Modified residue | 678 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 682 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in kidney cortex (at protein level).
Gene expression databases
Interaction
Subunit
Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6 (PubMed:12574114).
Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane (PubMed:12660155).
Interacts with calmodulin (PubMed:15123711).
Interacts with BSPRY, which results in its inactivation (PubMed:16380433).
Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane (PubMed:12660155).
Interacts with calmodulin (PubMed:15123711).
Interacts with BSPRY, which results in its inactivation (PubMed:16380433).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P69744 | Wnk4 Q80UE6 | 2 | EBI-538447, EBI-295378 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for repeat, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 38-68 | ANK 1 | ||||
Sequence: IWESPLLRAAKENDMCTLKKLQHDQNCDFRQ | ||||||
Repeat | 72-101 | ANK 2 | ||||
Sequence: LGETALHVAALYDNLDAAIMLMEAAPYLVT | ||||||
Repeat | 110-139 | ANK 3 | ||||
Sequence: VGQTALHIAVMNQNVNLVRALLARGASASA | ||||||
Repeat | 156-185 | ANK 4 | ||||
Sequence: YGEHPLSFAACVGSEEIVRLLIEHGADIRA | ||||||
Repeat | 189-222 | ANK 5 | ||||
Sequence: LGNTVLHILVLQPNKTFACQMYNLLLSYDGGDHL | ||||||
Repeat | 232-261 | ANK 6 | ||||
Sequence: QGLTPFKLAGVEGNTVMFQHLMQKRKRIQW | ||||||
Region | 591-595 | Interaction with S100A10 | ||||
Sequence: VATTV | ||||||
Region | 643-646 | Involved in Ca2+-dependent inactivation | ||||
Sequence: AFKS | ||||||
Region | 651-673 | Disordered | ||||
Sequence: EVQEQLSEKQPSGTETGTLARGS | ||||||
Compositional bias | 657-673 | Polar residues | ||||
Sequence: SEKQPSGTETGTLARGS | ||||||
Region | 693-723 | Involved in Ca2+-dependent inactivation | ||||
Sequence: RGWEILRRNTLGHLNLGLDPGEGDGEEIYQF |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length723
- Mass (Da)82,218
- Last updated2011-07-27 v2
- Checksum6668C67C47CBB443
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0A6YW69 | A0A0A6YW69_MOUSE | Trpv5 | 674 |
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 302 | in Ref. 2; AK085479 | ||||
Sequence: E → K | ||||||
Compositional bias | 657-673 | Polar residues | ||||
Sequence: SEKQPSGTETGTLARGS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF336378 EMBL· GenBank· DDBJ | AAM53408.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK085479 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BC110554 EMBL· GenBank· DDBJ | AAI10555.1 EMBL· GenBank· DDBJ | mRNA | ||
BC110555 EMBL· GenBank· DDBJ | AAI10556.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466533 EMBL· GenBank· DDBJ | EDL13503.1 EMBL· GenBank· DDBJ | Genomic DNA |