P69726 · VPR_HV1H2
- ProteinProtein Vpr
- Genevpr
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions.
During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest. Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the VPR carried into the virion. Additionally, VPR induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, VPR may also induce cell death to bystander cells.
Miscellaneous
HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell | |
Cellular Component | host cell nucleus | |
Cellular Component | host extracellular space | |
Cellular Component | virion component | |
Molecular Function | molecular adaptor activity | |
Biological Process | apoptotic process | |
Biological Process | biological process involved in interaction with host | |
Biological Process | DNA-templated transcription | |
Biological Process | monoatomic ion transmembrane transport | |
Biological Process | negative regulation of DNA repair | |
Biological Process | protein homooligomerization | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | symbiont entry into host cell | |
Biological Process | symbiont-mediated activation of host apoptosis | |
Biological Process | symbiont-mediated arrest of host cell cycle during G2/M transition | |
Biological Process | viral penetration into host nucleus |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein Vpr
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Pararnavirae > Artverviricota > Revtraviricetes > Ortervirales > Retroviridae > Orthoretrovirinae > Lentivirus > Human immunodeficiency virus type 1
- Virus hosts
Accessions
- Primary accessionP69726
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Incorporation into virion is dependent on p6 GAG sequences. Lacks a canonical nuclear localization signal, thus import into nucleus may function independently of the human importin pathway. Detected in high quantity in the serum and cerebrospinal fluid of AIDS patient.
Keywords
- Cellular component
Phenotypes & Variants
Keywords
- Disease
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000085451 | 1-96 | Protein Vpr | |||
Sequence: MEQAPEDQGPQREPHNEWTLELLEELKNEAVRHFPRIWLHGLGQHIYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS | ||||||
Modified residue | 79 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 94 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 96 | Phosphoserine; by host | ||||
Sequence: S |
Post-translational modification
Phosphorylated on several residues by host. These phosphorylations regulate VPR activity for the nuclear import of the HIV-1 pre-integration complex.
Keywords
- PTM
PTM databases
Interaction
Subunit
Homooligomer, may form homodimer. Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C-terminus of the P6gag protein. Interacts with host UNG. May interact with host RAD23A/HHR23A. Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediating ubiquitination of host proteins such as TERT and ZGPAT and arrest of the cell cycle in G2 phase.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length96
- Mass (Da)11,323
- Last updated2015-02-04 v2
- Checksum42892A4249E83D3E
Sequence caution
Keywords
- Technical term