P69514 · RDRP_CGMVS
- ProteinReplicase large subunit
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1648 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
The replicase large subunit is an RNA-dependent RNA polymerase active in viral RNA replication.
The replicase small subunit is a methyltransferase active in RNA capping and an RNA helicase. It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).
Miscellaneous
Readthrough of the terminator codon UAG occurs between codons for Lys-1144 and Gln-1146.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | mRNA methyltransferase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | DNA-templated transcription | |
Biological Process | mRNA modification | |
Biological Process | RNA processing | |
Biological Process | symbiont-mediated suppression of host innate immune response | |
Biological Process | viral RNA genome replication | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplicase large subunit
- EC number
- Alternative names
- Cleaved into 1 chains
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Virgaviridae > Tobamovirus > Cucumber green mottle mosaic virus
- Virus hosts
Accessions
- Primary accessionP69514
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000041173 | 1-1144 | Replicase small subunit | |||
Sequence: MANINEQINNQRDAAASGRNNLVSQLASKRVYDEAVRSLDHQDRRPKMNFSRVVSTEHTRLVTDAYPEFSISFTATKNSVHSLAGGLRLLELEYMMMQVPYGSPCYDIGGNYTQHLFKGRSYVHCCNPCLDLKDVARNVMYNDMITQHVQRHKGSGGCRPLPTFQIDAFRRYDSSPCAVTCSDVFQECSYDFGSGRDNHAVSLHSIYDIPYSSIGPALHRKNVRVCYAAFHFSEALLLGSPVGNLNSIGAQFRVDGDDVHFLFSEESTLHYTHSLENIKLIVMRTYFPADDRFVYIKEFMVKRVDTFFFRLVRADTHMLHKSVGHYSKSKSEYFALNTPPIFQDKATFSVWFPEAKRKVLIPKFELSRFLSGNVKISRMLVDADFVHTIINHISTYDNKALVWKNVQSFVESIRSRVIVNGVSVKSEWNVPVDQLTDISFSIFLLVKVRKVQIELMSDKVVIEARGLLRRFADSLKSAVEGLGDCVYDALVQTGWFDTSSDELKVLLPEPFMTFSDYLEGMYEADAKIERESVSELLASGDDLFKKIDEIRNNYSGVEFDVEKFQEFCKELNVNPMLIGHVIEAIFSQKAGVTVTGLGTLSPEMGASVALSSTSVDTCEDMDVTEDMEDIVLMADKSHSYMSPEMARWADVKYGNNKGALVEYKVGTSMTLPATWAEKGKAVLPLSGICVRKPQFSKPLDEEDDLRLSNMNFFKVSDLKLKKTITPVVYTGTIRERQMKNYIDYLSASLGSTLGNLERIVRSDWNGTEESMQTFGLYDCEKCKWLLLPAEKKHAWAVVLASDDTTRIIFLSYDESGSPIIDKKNWKRFAVCSETKVYSVIRSLEVLNKEAIVDPGVHITLVDGVPGCGKTAEIIARVNWKTDLVLTPGREAAAMIRRRACALHKSPVATNDNVRTFDSFVMNRKIFKFDAVYVDEGLMVHTGLLNFALKISGCKKAFVFGDAKQIPFINRVMNFDYPKELRTLIVDNVERRYVTHRCPRDVTSFLNTIYKAAVATTSPVVHSVKAIKVSGAGILRPELTKIKGKIITFTQSDKQSLIKSGYNDVNTVHEIQGETFEETAVVRATPTPIGLIARDSPHVLVALTRHTKAMVYYTVVFDAVTSIIADVEKVDQSILTMFATTVPTK | ||||||
Chain | PRO_0000041172 | 1-1648 | Replicase large subunit | |||
Sequence: MANINEQINNQRDAAASGRNNLVSQLASKRVYDEAVRSLDHQDRRPKMNFSRVVSTEHTRLVTDAYPEFSISFTATKNSVHSLAGGLRLLELEYMMMQVPYGSPCYDIGGNYTQHLFKGRSYVHCCNPCLDLKDVARNVMYNDMITQHVQRHKGSGGCRPLPTFQIDAFRRYDSSPCAVTCSDVFQECSYDFGSGRDNHAVSLHSIYDIPYSSIGPALHRKNVRVCYAAFHFSEALLLGSPVGNLNSIGAQFRVDGDDVHFLFSEESTLHYTHSLENIKLIVMRTYFPADDRFVYIKEFMVKRVDTFFFRLVRADTHMLHKSVGHYSKSKSEYFALNTPPIFQDKATFSVWFPEAKRKVLIPKFELSRFLSGNVKISRMLVDADFVHTIINHISTYDNKALVWKNVQSFVESIRSRVIVNGVSVKSEWNVPVDQLTDISFSIFLLVKVRKVQIELMSDKVVIEARGLLRRFADSLKSAVEGLGDCVYDALVQTGWFDTSSDELKVLLPEPFMTFSDYLEGMYEADAKIERESVSELLASGDDLFKKIDEIRNNYSGVEFDVEKFQEFCKELNVNPMLIGHVIEAIFSQKAGVTVTGLGTLSPEMGASVALSSTSVDTCEDMDVTEDMEDIVLMADKSHSYMSPEMARWADVKYGNNKGALVEYKVGTSMTLPATWAEKGKAVLPLSGICVRKPQFSKPLDEEDDLRLSNMNFFKVSDLKLKKTITPVVYTGTIRERQMKNYIDYLSASLGSTLGNLERIVRSDWNGTEESMQTFGLYDCEKCKWLLLPAEKKHAWAVVLASDDTTRIIFLSYDESGSPIIDKKNWKRFAVCSETKVYSVIRSLEVLNKEAIVDPGVHITLVDGVPGCGKTAEIIARVNWKTDLVLTPGREAAAMIRRRACALHKSPVATNDNVRTFDSFVMNRKIFKFDAVYVDEGLMVHTGLLNFALKISGCKKAFVFGDAKQIPFINRVMNFDYPKELRTLIVDNVERRYVTHRCPRDVTSFLNTIYKAAVATTSPVVHSVKAIKVSGAGILRPELTKIKGKIITFTQSDKQSLIKSGYNDVNTVHEIQGETFEETAVVRATPTPIGLIARDSPHVLVALTRHTKAMVYYTVVFDAVTSIIADVEKVDQSILTMFATTVPTKXQLMQNSLYVHRNIFLPVSKTGFYTDMQEFYDRCLPGNSFVLNDFDAVTMRLRDNEFNLQPCRLTLSNLDPVPALIKNEAQNFLIPVLRTACERPRIPGLLENLVAMIKRNMNTPDLAGTVDITNMSISIVDNFFSSFVRDEVLLDHLDCVRASSIQSFSDWFSCQPTSAVGQLANFNFIDLPAFDTYMHMIKRQPKSRLDTSIQSEYPALQTIVYHPKVVNAVFGPVFKYLTTKFLSMVDSSKFFFYTRKKPEDLQEFFSDLSSHSDYEILELDVSKYDKSQSDFHFSIEMAIWEKLGLDDILAWMWSMGHKRTILQDFQAGIKTLIYYQRKSGDVTTFIGNTFIIAACVASMLPLDKCFKASFCGDDSLIYLPKGLEYPDIQATANLVWNFEAKLFRKKYGYFCGKYIIHHANGCIVYPDPLKLISKLGNKSLVGYEHVEEFRISLLDVAHSLFNGAYFHLLDDAIHELFPNAGGCSFVINCLCKYLSDKRLFRSLYIDVSK |
Interaction
Subunit
Heterodimer of a large and a small subunit.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-20 | Disordered | ||||
Sequence: MANINEQINNQRDAAASGRN | ||||||
Domain | 72-287 | Alphavirus-like MT | ||||
Sequence: SFTATKNSVHSLAGGLRLLELEYMMMQVPYGSPCYDIGGNYTQHLFKGRSYVHCCNPCLDLKDVARNVMYNDMITQHVQRHKGSGGCRPLPTFQIDAFRRYDSSPCAVTCSDVFQECSYDFGSGRDNHAVSLHSIYDIPYSSIGPALHRKNVRVCYAAFHFSEALLLGSPVGNLNSIGAQFRVDGDDVHFLFSEESTLHYTHSLENIKLIVMRTYF | ||||||
Domain | 831-991 | +RNA virus helicase ATP-binding | ||||
Sequence: CSETKVYSVIRSLEVLNKEAIVDPGVHITLVDGVPGCGKTAEIIARVNWKTDLVLTPGREAAAMIRRRACALHKSPVATNDNVRTFDSFVMNRKIFKFDAVYVDEGLMVHTGLLNFALKISGCKKAFVFGDAKQIPFINRVMNFDYPKELRTLIVDNVERR | ||||||
Domain | 992-1144 | +RNA virus helicase C-terminal | ||||
Sequence: YVTHRCPRDVTSFLNTIYKAAVATTSPVVHSVKAIKVSGAGILRPELTKIKGKIITFTQSDKQSLIKSGYNDVNTVHEIQGETFEETAVVRATPTPIGLIARDSPHVLVALTRHTKAMVYYTVVFDAVTSIIADVEKVDQSILTMFATTVPTK | ||||||
Domain | 1413-1526 | RdRp catalytic | ||||
Sequence: YEILELDVSKYDKSQSDFHFSIEMAIWEKLGLDDILAWMWSMGHKRTILQDFQAGIKTLIYYQRKSGDVTTFIGNTFIIAACVASMLPLDKCFKASFCGDDSLIYLPKGLEYPD |
Sequence similarities
Belongs to the tobamovirus RNA-directed RNA polymerase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,648
- Mass (Da)186,551
- Last updated2005-03-15 v1
- Checksum1D1AFFEEE7B65595
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D12505 EMBL· GenBank· DDBJ | BAA18895.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
D12505 EMBL· GenBank· DDBJ | BAA18896.1 EMBL· GenBank· DDBJ | Genomic RNA |