P68183 · MALG_ECOLI
- ProteinMaltose/maltodextrin transport system permease protein MalG
- GenemalG
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids296 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Probably responsible for the translocation of the substrate across the membrane.
Miscellaneous
When MalF EAA loop mutations are made concomitantly with MalG EAA loop mutations, a complete loss of transport and complex formation is observed. This suggests that the MalF-MalG interaction may be important for the proper assembly and also for the correct function of the transporter.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | ATP-binding cassette (ABC) transporter complex | |
Cellular Component | enzyme IIA-maltose transporter complex | |
Cellular Component | maltose transport complex | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | ABC-type maltose transporter activity | |
Biological Process | maltodextrin transmembrane transport | |
Biological Process | maltose transport | |
Biological Process | negative regulation of maltose transport | |
Biological Process | negative regulation of transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMaltose/maltodextrin transport system permease protein MalG
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP68183
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-18 | Cytoplasmic | ||||
Sequence: MAMVQPKSQKARLFITHL | ||||||
Transmembrane | 19-39 | Helical | ||||
Sequence: LLLLFIAAIMFPLLMVVAISL | ||||||
Topological domain | 40-81 | Periplasmic | ||||
Sequence: RQGNFATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPV | ||||||
Transmembrane | 82-102 | Helical | ||||
Sequence: LLWLWNSVKVAGISAIGIVAL | ||||||
Topological domain | 103-123 | Cytoplasmic | ||||
Sequence: STTCAYAFARMRFPGKATLLK | ||||||
Transmembrane | 124-144 | Helical | ||||
Sequence: GMLIFQMFPAVLSLVALYALF | ||||||
Topological domain | 145-150 | Periplasmic | ||||
Sequence: DRLGEY | ||||||
Transmembrane | 151-171 | Helical | ||||
Sequence: IPFIGLNTHGGVIFAYLGGIA | ||||||
Topological domain | 172-204 | Cytoplasmic | ||||
Sequence: LHVWTIKGYFETIDSSLEEAAALDGATPWQAFR | ||||||
Transmembrane | 205-225 | Helical | ||||
Sequence: LVLLPLSVPILAVVFILSFIA | ||||||
Topological domain | 226-259 | Periplasmic | ||||
Sequence: AITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYL | ||||||
Transmembrane | 260-280 | Helical | ||||
Sequence: WGDFAAAAVMSALPITIVFLL | ||||||
Topological domain | 281-296 | Cytoplasmic | ||||
Sequence: AQRWLVNGLTAGGVKG |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 190 | Reduction of transport rate. | ||||
Sequence: E → A, C, K, or L | ||||||
Mutagenesis | 192 | Loss of transport and MalK dissociation from the membrane. | ||||
Sequence: A → D, S, or L | ||||||
Mutagenesis | 196 | No effect. | ||||
Sequence: G → A | ||||||
Mutagenesis | 196 | Loss of transport and MalK dissociation from the membrane. | ||||
Sequence: G → P | ||||||
Mutagenesis | 209 | No effect. | ||||
Sequence: P → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000060081 | 1-296 | Maltose/maltodextrin transport system permease protein MalG | |||
Sequence: MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNFATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVALSTTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHGGVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITIVFLLAQRWLVNGLTAGGVKG |
Proteomic databases
Interaction
Subunit
The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE). Protein stability and stable complex formation require YidC.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P68183 | malF P02916 | 5 | EBI-6400985, EBI-1118919 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 85-281 | ABC transmembrane type-1 | ||||
Sequence: LWNSVKVAGISAIGIVALSTTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHGGVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITIVFLLA |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length296
- Mass (Da)32,225
- Last updated1988-04-01 v1
- ChecksumE54418E91E344A29
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X02871 EMBL· GenBank· DDBJ | CAA26628.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00006 EMBL· GenBank· DDBJ | AAC43126.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC77002.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE78034.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
J02812 EMBL· GenBank· DDBJ | AAA79015.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X06663 EMBL· GenBank· DDBJ | CAA29861.1 EMBL· GenBank· DDBJ | Genomic DNA |