P63479 · ALR1_STAAM
- ProteinAlanine racemase 1
- Genealr1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids382 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the interconversion of L-alanine and D-alanine.
Catalytic activity
- L-alanine = D-alanine
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.89 mM | D-alanine | 30 | ||||
2.77 mM | L-alanine | 30 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
91 μmol/min/mg | 30 | toward D-alanine | |||
250 μmol/min/mg | 30 | toward L-alanine |
Pathway
Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 39 | Proton acceptor; specific for D-alanine | ||||
Sequence: K | ||||||
Binding site | 138 | substrate | ||||
Sequence: R | ||||||
Active site | 265 | Proton acceptor; specific for L-alanine | ||||
Sequence: Y | ||||||
Binding site | 312 | substrate | ||||
Sequence: M |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | alanine racemase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | D-alanine biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlanine racemase 1
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionP63479
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000114568 | 1-382 | Alanine racemase 1 | |||
Sequence: MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQLVFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTTIALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK | ||||||
Modified residue | 39 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length382
- Mass (Da)42,809
- Last updated2004-10-11 v1
- ChecksumA46B7E55F5A65E41
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000017 EMBL· GenBank· DDBJ | BAB58232.1 EMBL· GenBank· DDBJ | Genomic DNA |