P63072 · UBD_MOUSE

  • Protein
    Ubiquitin D
  • Gene
    Ubd
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner. Probably functions as a survival factor. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases.

Features

Showing features for site.

116220406080100120140160
TypeIDPosition(s)Description
Site161-162Activation by thioester intermediate formation with UBA6

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentaggresome
Cellular Componentcytoplasm
Cellular Componentfibrillar center
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular Functionproteasome binding
Biological Processaggresome assembly
Biological Processmyeloid dendritic cell differentiation
Biological Processpositive regulation of apoptotic process
Biological Processpositive regulation of canonical NF-kappaB signal transduction
Biological Processprotein modification by small protein conjugation
Biological Processprotein ubiquitination
Biological Processregulation of mitotic cell cycle phase transition
Biological Processresponse to tumor necrosis factor
Biological Processresponse to type II interferon
Biological Processubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin D
  • Alternative names
    • Diubiquitin
    • Ubiquitin-like protein FAT10

Gene names

    • Name
      Ubd
    • Synonyms
      Fat10

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P63072
  • Secondary accessions
    • Q4FJZ5
    • Q9WV10

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm
Note: Accumulates in aggresomes under proteasome inhibition conditions.

Keywords

Phenotypes & Variants

Disruption phenotype

Spontaneous sporadic apoptotic death. Higher sensitivity toward endotoxin challenge. Abrogated TNF-alpha-induced NF-kappa-B activation and reduced induction of NF-kappa-B-regulated genes. Impaired TNF-alpha-induced I-kappa-B-alpha degradation and nuclear translocation of p65 in RTECs. Reduced expression of LMP2.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis161-162Impaired conjugation of target proteins.

Variants

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The viewer provides 13 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001148941-162Ubiquitin D

Post-translational modification

Can be acetylated.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Mostly expressed in thymus and intestine.

Induction

Rapidly degraded by the proteasome. Cell-cycle regulation with highest expression during the S-phase (at protein level). Over expressed in hepatocytes by drug injury (e.g. DDC; diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate). Inducible by the pro-inflammatory cytokines tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg).

Gene expression databases

Interaction

Subunit

Interacts directly with the 26S proteasome. The interaction with NUB1 via the N-terminal ubiquitin domain facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates its own degradation and that of its conjugates. Interacts (via ubiquitin-like 1 domain) with the spindle checkpoint protein MAD2L1 during mitosis. Present in aggresomes of proteasome inhibited cells. Interacts with HDAC6 under proteasome impairment conditions (By similarity).
Forms a thioester with UBA6 in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg) (PubMed:17889673).
Interacts with SQSTM1 and TP53/p53 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain3-78Ubiquitin-like 1
Domain87-160Ubiquitin-like 2

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    162
  • Mass (Da)
    18,376
  • Last updated
    2004-09-13 v1
  • Checksum
    6107D8604F96455F
MASVRTCVVRSDQWRLMTFETTENDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRKLSSYGIDKETTIHLTLKVVKPSDEELPLFLVESKNEGQRHLLRVRRSSSVAQVKEMIESVTSVIPKKQVVNCNGKKLEDGKIMADYNIKSGSLLFLTTHCTGG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF314088
EMBL· GenBank· DDBJ
AAG27477.1
EMBL· GenBank· DDBJ
Genomic DNA
AK008116
EMBL· GenBank· DDBJ
BAB25471.1
EMBL· GenBank· DDBJ
mRNA
AK008552
EMBL· GenBank· DDBJ
BAB25738.1
EMBL· GenBank· DDBJ
mRNA
CT010257
EMBL· GenBank· DDBJ
CAJ18465.1
EMBL· GenBank· DDBJ
mRNA
AL078630
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC027627
EMBL· GenBank· DDBJ
AAH27627.2
EMBL· GenBank· DDBJ
mRNA
BC036383
EMBL· GenBank· DDBJ
AAH36383.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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