P62869 · ELOB_MOUSE

  • Protein
    Elongin-B
  • Gene
    Elob
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites (By similarity).
Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity).
In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (PubMed:27863225, PubMed:27863226).
Core component of multiple cullin-2 and cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes), which mediate the ubiquitination of target proteins (PubMed:33590678).
By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes (By similarity).
Component the von Hippel-Lindau ubiquitination complex CBC(VHL) (By similarity).
A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (By similarity).
The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (By similarity).
As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A (By similarity).
ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (PubMed:33590678).

Pathway

Protein modification; protein ubiquitination.

GO annotations

AspectTerm
Cellular ComponentCul2-RING ubiquitin ligase complex
Cellular ComponentCul5-RING ubiquitin ligase complex
Cellular Componentcytosol
Cellular Componentelongin complex
Cellular Componenttranscription regulator complex
Cellular ComponentVCB complex
Molecular Functionprotein-containing complex binding
Molecular Functiontranscription coactivator activity
Molecular Functiontranscription corepressor binding
Molecular Functionubiquitin protein ligase binding
Biological Processprotein ubiquitination
Biological Processtarget-directed miRNA degradation
Biological Processtranscription elongation by RNA polymerase II
Biological Processtranscription initiation at RNA polymerase II promoter

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Elongin-B
  • Short names
    EloB
  • Alternative names
    • Elongin 18 kDa subunit
    • RNA polymerase II transcription factor SIII subunit B
    • SIII p18
    • Transcription elongation factor B polypeptide 2

Gene names

    • Name
      Elob
    • Synonyms
      Tceb2

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P62869
  • Secondary accessions
    • Q63529
    • Q80W20

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00001149151-118Elongin-B
Modified residue84Phosphothreonine
Modified residue108Phosphoserine
Modified residue111Phosphoserine

Keywords

Proteomic databases

2D gel databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit (PubMed:16498413).
The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226).
Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL or WSB1 (PubMed:10051596, PubMed:11384984, PubMed:23897481, PubMed:33590678).
As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1 (By similarity).
May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5 (By similarity).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-79Ubiquitin-like
Region91-118Disordered

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    118
  • Mass (Da)
    13,170
  • Last updated
    2004-08-16 v1
  • Checksum
    BA702F24CEC0FC02
MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPEEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALRIEPFSSPPELPDVMKPQDSGGSANEQAVQ

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A3B2WBM3A0A3B2WBM3_MOUSEElob90

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK019358
EMBL· GenBank· DDBJ
BAB31677.1
EMBL· GenBank· DDBJ
mRNA
AK002223
EMBL· GenBank· DDBJ
BAB21946.1
EMBL· GenBank· DDBJ
mRNA
AK002868
EMBL· GenBank· DDBJ
BAB22417.1
EMBL· GenBank· DDBJ
mRNA
AK003277
EMBL· GenBank· DDBJ
BAB22684.1
EMBL· GenBank· DDBJ
mRNA
AK008477
EMBL· GenBank· DDBJ
BAB25690.1
EMBL· GenBank· DDBJ
mRNA
AK008534
EMBL· GenBank· DDBJ
BAB25727.1
EMBL· GenBank· DDBJ
mRNA
AK012254
EMBL· GenBank· DDBJ
BAB28121.1
EMBL· GenBank· DDBJ
mRNA
AK019181
EMBL· GenBank· DDBJ
BAB31590.1
EMBL· GenBank· DDBJ
mRNA
AK019218
EMBL· GenBank· DDBJ
BAB31608.1
EMBL· GenBank· DDBJ
mRNA
BC051927
EMBL· GenBank· DDBJ
AAH51927.2
EMBL· GenBank· DDBJ
mRNA
BC056983
EMBL· GenBank· DDBJ
AAH56983.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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