P62869 · ELOB_MOUSE
- ProteinElongin-B
- GeneElob
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids118 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites (By similarity).
Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity).
In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (PubMed:27863225, PubMed:27863226).
Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity).
In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (PubMed:27863225, PubMed:27863226).
Core component of multiple cullin-2 and cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes), which mediate the ubiquitination of target proteins (PubMed:33590678).
By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes (By similarity).
Component the von Hippel-Lindau ubiquitination complex CBC(VHL) (By similarity).
A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (By similarity).
The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (By similarity).
As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A (By similarity).
ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (PubMed:33590678).
By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes (By similarity).
Component the von Hippel-Lindau ubiquitination complex CBC(VHL) (By similarity).
A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (By similarity).
The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (By similarity).
As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A (By similarity).
ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (PubMed:33590678).
Pathway
Protein modification; protein ubiquitination.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Cul2-RING ubiquitin ligase complex | |
Cellular Component | Cul5-RING ubiquitin ligase complex | |
Cellular Component | cytosol | |
Cellular Component | elongin complex | |
Cellular Component | transcription regulator complex | |
Cellular Component | VCB complex | |
Molecular Function | protein-containing complex binding | |
Molecular Function | transcription coactivator activity | |
Molecular Function | transcription corepressor binding | |
Molecular Function | ubiquitin protein ligase binding | |
Biological Process | protein ubiquitination | |
Biological Process | target-directed miRNA degradation | |
Biological Process | transcription elongation by RNA polymerase II | |
Biological Process | transcription initiation at RNA polymerase II promoter |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameElongin-B
- Short namesEloB
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP62869
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000114915 | 1-118 | Elongin-B | |||
Sequence: MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPEEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALRIEPFSSPPELPDVMKPQDSGGSANEQAVQ | ||||||
Modified residue | 84 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 108 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 111 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
2D gel databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit (PubMed:16498413).
The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226).
Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL or WSB1 (PubMed:10051596, PubMed:11384984, PubMed:23897481, PubMed:33590678).
As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1 (By similarity).
May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5 (By similarity).
The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226).
Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL or WSB1 (PubMed:10051596, PubMed:11384984, PubMed:23897481, PubMed:33590678).
As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1 (By similarity).
May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5 (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-79 | Ubiquitin-like | ||||
Sequence: MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPEEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAF | ||||||
Region | 91-118 | Disordered | ||||
Sequence: EPFSSPPELPDVMKPQDSGGSANEQAVQ |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length118
- Mass (Da)13,170
- Last updated2004-08-16 v1
- ChecksumBA702F24CEC0FC02
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A3B2WBM3 | A0A3B2WBM3_MOUSE | Elob | 90 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK019358 EMBL· GenBank· DDBJ | BAB31677.1 EMBL· GenBank· DDBJ | mRNA | ||
AK002223 EMBL· GenBank· DDBJ | BAB21946.1 EMBL· GenBank· DDBJ | mRNA | ||
AK002868 EMBL· GenBank· DDBJ | BAB22417.1 EMBL· GenBank· DDBJ | mRNA | ||
AK003277 EMBL· GenBank· DDBJ | BAB22684.1 EMBL· GenBank· DDBJ | mRNA | ||
AK008477 EMBL· GenBank· DDBJ | BAB25690.1 EMBL· GenBank· DDBJ | mRNA | ||
AK008534 EMBL· GenBank· DDBJ | BAB25727.1 EMBL· GenBank· DDBJ | mRNA | ||
AK012254 EMBL· GenBank· DDBJ | BAB28121.1 EMBL· GenBank· DDBJ | mRNA | ||
AK019181 EMBL· GenBank· DDBJ | BAB31590.1 EMBL· GenBank· DDBJ | mRNA | ||
AK019218 EMBL· GenBank· DDBJ | BAB31608.1 EMBL· GenBank· DDBJ | mRNA | ||
BC051927 EMBL· GenBank· DDBJ | AAH51927.2 EMBL· GenBank· DDBJ | mRNA | ||
BC056983 EMBL· GenBank· DDBJ | AAH56983.1 EMBL· GenBank· DDBJ | mRNA |