P62834 · RAP1A_HUMAN
- ProteinRas-related protein Rap-1A
- GeneRAP1A
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids184 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed:17916086).
Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567).
May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions
Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567).
May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Activity regulation
Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRas-related protein Rap-1A
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP62834
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Note: Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity).
Colocalized with RAPGEF2 in the perinuclear region
Colocalized with RAPGEF2 in the perinuclear region
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 139 variants from UniProt as well as other sources including ClinVar and dbSNP.
Keywords
- Disease
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue, lipidation, propeptide.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000030199 | 1-181 | UniProt | Ras-related protein Rap-1A | |||
Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSC | |||||||
Modified residue (large scale data) | 11 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 39 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 181 | UniProt | Cysteine methyl ester | ||||
Sequence: C | |||||||
Lipidation | 181 | UniProt | S-geranylgeranyl cysteine | ||||
Sequence: C | |||||||
Propeptide | PRO_0000030200 | 182-184 | UniProt | Removed in mature form | |||
Sequence: LLL |
Keywords
- PTM
Proteomic databases
2D gel databases
PTM databases
Expression
Interaction
Subunit
Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A (PubMed:17916086).
Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1 (PubMed:17916086).
Found in a complex composed of CDH1, RAP1A and PKP3; PKP3 acts as a scaffold protein within the complex, the complex is required for CDH1 localization to mature desmosome cell junctions (PubMed:25208567).
In its GTP-bound form interacts with PLCE1 and RADIL (PubMed:11022048, PubMed:12444546, PubMed:17704304).
Interacts with SGSM1, SGSM2 and SGSM3 (PubMed:17509819).
Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain) (PubMed:10608844, PubMed:11359771).
Interacts with TBC1D21 (By similarity).
Interacts with RAP1GDS1 (PubMed:20709748).
Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1 (PubMed:17916086).
Found in a complex composed of CDH1, RAP1A and PKP3; PKP3 acts as a scaffold protein within the complex, the complex is required for CDH1 localization to mature desmosome cell junctions (PubMed:25208567).
In its GTP-bound form interacts with PLCE1 and RADIL (PubMed:11022048, PubMed:12444546, PubMed:17704304).
Interacts with SGSM1, SGSM2 and SGSM3 (PubMed:17509819).
Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain) (PubMed:10608844, PubMed:11359771).
Interacts with TBC1D21 (By similarity).
Interacts with RAP1GDS1 (PubMed:20709748).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P62834 | RAF1 P04049 | 2 | EBI-491414, EBI-365996 | |
BINARY | P62834 | RASSF5 Q8WWW0-2 | 3 | EBI-491414, EBI-960502 | |
XENO | P62834 | Rassf5 Q5EBH1 | 3 | EBI-491414, EBI-960530 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 32-40 | Effector region | ||||
Sequence: YDPTIEDSY |
Sequence similarities
Belongs to the small GTPase superfamily. Ras family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length184
- Mass (Da)20,987
- Last updated2004-08-16 v1
- Checksum42C39290C98E0A92
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X12533 EMBL· GenBank· DDBJ | CAA31051.1 EMBL· GenBank· DDBJ | mRNA | ||
M22995 EMBL· GenBank· DDBJ | AAA36150.1 EMBL· GenBank· DDBJ | mRNA | ||
AF493912 EMBL· GenBank· DDBJ | AAM12626.1 EMBL· GenBank· DDBJ | mRNA | ||
AL049557 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC014086 EMBL· GenBank· DDBJ | AAH14086.1 EMBL· GenBank· DDBJ | mRNA |