P62834 · RAP1A_HUMAN

  • Protein
    Ras-related protein Rap-1A
  • Gene
    RAP1A
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed:17916086).
Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567).
May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions

Catalytic activity

Activity regulation

Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site10-18GTP (UniProtKB | ChEBI)
Binding site29-35GTP (UniProtKB | ChEBI)
Binding site60GTP (UniProtKB | ChEBI)
Binding site116-119GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentanchoring junction
Cellular Componentcell junction
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentearly endosome
Cellular Componentendosome membrane
Cellular Componentextracellular exosome
Cellular Componentglutamatergic synapse
Cellular Componentguanyl-nucleotide exchange factor complex
Cellular Componentlate endosome
Cellular Componentneuron projection
Cellular Componentperinuclear region of cytoplasm
Cellular Componentplasma membrane
Cellular Componentpresynapse
Cellular Componentspecific granule membrane
Cellular Componentsperm midpiece
Molecular FunctionG protein activity
Molecular FunctionGDP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionguanyl-nucleotide exchange factor activity
Molecular Functionprotein-containing complex binding
Molecular Functionsmall GTPase binding
Biological Processcellular response to cAMP
Biological Processcellular response to nerve growth factor stimulus
Biological Processestablishment of endothelial barrier
Biological Processnegative regulation of synaptic vesicle exocytosis
Biological Processnerve growth factor signaling pathway
Biological Processnervous system development
Biological Processpositive regulation of ERK1 and ERK2 cascade
Biological Processpositive regulation of GTPase activity
Biological Processpositive regulation of neuron projection development
Biological Processpositive regulation of protein kinase activity
Biological Processpositive regulation of vasculogenesis
Biological Processprotein localization to plasma membrane
Biological ProcessRap protein signal transduction
Biological Processregulation of cell junction assembly
Biological Processregulation of neurotransmitter receptor localization to postsynaptic specialization membrane
Biological Processsynaptic vesicle exocytosis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ras-related protein Rap-1A
  • EC number
  • Alternative names
    • C21KG
    • G-22K
    • GTP-binding protein smg p21A
    • Ras-related protein Krev-1

Gene names

    • Name
      RAP1A
    • Synonyms
      KREV1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    P62834
  • Secondary accessions
    • P10113

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor
Cytoplasm
Cell junction
Early endosome
Note: Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity).
Colocalized with RAPGEF2 in the perinuclear region

Keywords

Disease & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 139 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Keywords

Organism-specific databases

Miscellaneous

Chemistry

Genetic variation databases

PTM/Processing

Features

Showing features for chain, modified residue (large scale data), modified residue, lipidation, propeptide.

TypeIDPosition(s)SourceDescription
ChainPRO_00000301991-181UniProt
Modified residue (large scale data)11PRIDEPhosphoserine
Modified residue (large scale data)39PRIDEPhosphoserine
Modified residue181UniProtCysteine methyl ester
Lipidation181UniProtS-geranylgeranyl cysteine
PropeptidePRO_0000030200182-184UniProtRemoved in mature form

Keywords

Proteomic databases

2D gel databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A (PubMed:17916086).
Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1 (PubMed:17916086).
Found in a complex composed of CDH1, RAP1A and PKP3; PKP3 acts as a scaffold protein within the complex, the complex is required for CDH1 localization to mature desmosome cell junctions (PubMed:25208567).
In its GTP-bound form interacts with PLCE1 and RADIL (PubMed:11022048, PubMed:12444546, PubMed:17704304).
Interacts with SGSM1, SGSM2 and SGSM3 (PubMed:17509819).
Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain) (PubMed:10608844, PubMed:11359771).
Interacts with TBC1D21 (By similarity).
Interacts with RAP1GDS1 (PubMed:20709748).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P62834RAF1 P040492EBI-491414, EBI-365996
BINARY P62834RASSF5 Q8WWW0-23EBI-491414, EBI-960502
XENO P62834Rassf5 Q5EBH13EBI-491414, EBI-960530

Protein-protein interaction databases

Chemistry

Miscellaneous

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif32-40Effector region

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    184
  • Mass (Da)
    20,987
  • Last updated
    2004-08-16 v1
  • Checksum
    42C39290C98E0A92
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X12533
EMBL· GenBank· DDBJ
CAA31051.1
EMBL· GenBank· DDBJ
mRNA
M22995
EMBL· GenBank· DDBJ
AAA36150.1
EMBL· GenBank· DDBJ
mRNA
AF493912
EMBL· GenBank· DDBJ
AAM12626.1
EMBL· GenBank· DDBJ
mRNA
AL049557
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC014086
EMBL· GenBank· DDBJ
AAH14086.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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