P62620 · ISPG_ECOLI
- Protein4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
- GeneispG
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate, using flavodoxin as the reducing agent.
Catalytic activity
- (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
pH Dependence
Optimum pH is 9.
Pathway
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) | |
Molecular Function | 4-hydroxy-3-methylbut-2-enyl-diphosphate synthase activity (flavodoxin) | |
Molecular Function | iron ion binding | |
Molecular Function | metal ion binding | |
Biological Process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP62620
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 322 | Loss of activity. | ||||
Sequence: G → D | ||||||
Mutagenesis | 328 | Loss of activity. | ||||
Sequence: G → D | ||||||
Mutagenesis | 335 | Loss of activity. | ||||
Sequence: R → C |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000190572 | 1-372 | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) | |||
Sequence: MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK |
Proteomic databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P62620 | ecpB P77188 | 2 | EBI-550338, EBI-1113651 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)40,684
- Last updated2004-07-19 v1
- Checksum3666FD83D5CD4F9F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X64451 EMBL· GenBank· DDBJ | CAA45783.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY033515 EMBL· GenBank· DDBJ | AAK53460.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75568.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16402.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
M11843 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |