P62500 · T22D1_MOUSE

  • Protein
    TSC22 domain family protein 1
  • Gene
    Tsc22d1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Transcriptional repressor (By similarity).
Acts on the C-type natriuretic peptide (CNP) promoter (By similarity).
Acts to promote CASP3-mediated apoptosis (By similarity).
Positively regulates TGF-beta signaling by interacting with SMAD7 which inhibits binding of SMAD7 to TGFBR1, preventing recruitment of SMURF ubiquitin ligases to TGFBR1 and inhibiting SMURF-mediated ubiquitination and degradation of TGFBR1 (By similarity).
Contributes to enhancement of TGF-beta signaling by binding to and modulating the transcription activator activity of SMAD4 (By similarity).
Promotes TGF-beta-induced transcription of COL1A2; via its interaction with TFE3 at E-boxes in the gene proximal promoter (PubMed:20713358).
Plays a role in the repression of hematopoietic precursor cell growth (PubMed:19329776).
Promotes IL2 deprivation-induced apoptosis in T-lymphocytes, via repression of TSC22D3/GILZ transcription and activation of the caspase cascade (PubMed:26752201).

Isoform 1

May act to negatively regulate TGFB3 signaling and thereby inhibit cell death in mammary gland cells.

Isoform 2

Positively regulates cell death in response to TGFB3 during mammary gland involution.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentmitochondrion
Cellular Componentnucleus
Cellular Componentplasma membrane
Biological Processnegative regulation of apoptotic process
Biological Processnegative regulation of hematopoietic stem cell proliferation
Biological Processnegative regulation of programmed cell death
Biological Processpositive regulation of apoptotic process
Biological Processpositive regulation of cell population proliferation
Biological Processpositive regulation of programmed cell death
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processpositive regulation of transforming growth factor beta receptor signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    TSC22 domain family protein 1
  • Alternative names
    • Regulatory protein TSC-22
    • TGFB-stimulated clone 22 homolog
    • TSC22-related inducible leucine zipper 1b
    • Transforming growth factor beta-1-induced transcript 4 protein

Gene names

    • Name
      Tsc22d1
    • Synonyms
      Kiaa1994
      , Tgfb1i4
      , Tilz1b, Tsc22

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P62500
  • Secondary accessions
    • Q00992
    • Q69Z44
    • Q6PDK1
    • Q9EQN4

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Cell membrane
; Peripheral membrane protein

Isoform 1

Cytoplasm
Nucleus
Mitochondrion

Isoform 2

Cytoplasm
Nucleus
Mitochondrion

Keywords

Phenotypes & Variants

Disruption phenotype

Increase in DNA synthesis in hematopoietic precursor cells from bone marrow, these cells have a greater ability to proliferate and repopulate an irradiated recipient.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1024Abolishes homodimerization; when associated with A-1030.
Mutagenesis1030Abolishes homodimerization; when associated with A-1024.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002193661-1077TSC22 domain family protein 1
Modified residue263Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in bone marrow cells (at protein level) (PubMed:19329776).
Expressed in T-cells (PubMed:26752201).
Expressed in the brain (PubMed:19329776).

Isoform 1

Expressed in the myoepithelial cells of the mammary gland ducts and alveoli, expression is consistent throughout pregnancy, lactation and involution (at protein level) (PubMed:19745830).
Expressed in the cortex, medulla and papilla of the kidney (PubMed:17147695).

Isoform 2

Expressed in the myoepithelial cells of the mammary gland, expression significantly increases in the secretory luminal epithelium of the mammary gland at the initiation of involution, with levels decreasing from day 3 of involution onwards (at protein level) (PubMed:19745830).
Expressed in the cortex, medulla and papilla of the kidney (PubMed:17147695).

Induction

Induced by Tgfb1, Pparg and other growth factors (PubMed:12468551, PubMed:1587811).
Induced by Tgfb3 in mammary gland cells (PubMed:19745830).
Induced by TGF-beta via up-regulation of Ybx1 regulator miR-216a which decreases Ybx1 abundance and binding of Ybx1 to Tsc22d1 transcripts, thereby increasing Tsc22d1 translation (PubMed:20713358).
Induced in the glomeruli of a diabetic mouse model (PubMed:20713358).

Gene expression databases

Interaction

Subunit

Forms homodimers (PubMed:12468551).
Forms heterodimers (By similarity).
Component of a complex composed of TSC22D1 (via N-terminus), TGFBR1 and TGFBR2; the interaction between TSC22D1 and TGFBR1 is inhibited by SMAD7 and promoted by TGFB1 (By similarity).
Interacts with SMAD7; the interaction requires TGF-beta and the interaction is inhibited by TGFBR1 (By similarity).
Interacts with TPT1/fortilin; interaction results in the destabilization of TSC22D1 protein and prevents TSC22D1-mediated apoptosis (By similarity).
Interacts with SMAD4 (via N-terminus) (By similarity).
Interacts with ACVRL1/ALK1, ACVR1/ALK2, BMPR1A/ALK3, ACVR1B/ALK4, BMPR1B/ALK6, ACVR2A/ACTRII, and BMPR2 (By similarity).
Interacts with SMAD6 (By similarity).
Interacts with TFE3; the interaction is enhanced in the presence of TGF-beta (PubMed:20713358).

Isoform 1

Forms a heterodimer with TSC22D4/THG1.

Isoform 2

Forms a heterodimer with TSC22D4/THG1 (By similarity).
Interacts with histone H1-2 (By similarity).
Interacts with GNL3 (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P62500Tsc22d4 Q9EQN32EBI-8296837, EBI-7821198

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-98Required for interaction with TGFBR1 and promotion of TGF-beta signaling
Region22-112Disordered
Compositional bias59-84Pro residues
Compositional bias85-99Polar residues
Region125-283Disordered
Compositional bias172-188Polar residues
Compositional bias216-240Basic residues
Compositional bias458-489Polar residues
Region458-492Disordered
Region842-874Disordered
Region909-947Disordered
Compositional bias930-947Polar residues
Region1010-1031Leucine-zipper
Region1042-1077Disordered

Sequence similarities

Belongs to the TSC-22/Dip/Bun family.

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

P62500-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    TSC22D1-1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    1,077
  • Mass (Da)
    109,811
  • Last updated
    2008-09-02 v2
  • Checksum
    35749C0C1CA1E1B6
MHQPPESTAAAAAAADISARKMAHPAMFPRRGSGGGSASALNAAGTGVSGAAPSSEDFPPPSLLQPPPPAASSTQGPQPPPPQSLNLLSQAQLQGQPLAPGGTQMKKKSGFQITSVTPAQISASISSNNSIAEDTESYDDLDESHTEDLSSSEILDVSLSRATDLGEPERSSSEETLNNFQEAETPGAVSPNQPHLPQPHLPHLPQQNVVINGNAHPHHLHHHHHPHHGHHLHHGHHHSSHAAVAGPSIPGGPPSSPVSRKLSTTGSSDGGVPVAPPPAVPSSGLPASVMTNIRTPSTTGSLGINSVTGTSATNNVNIAAVGSFSPSVTNSVHGNANINTSNIPNAASISGGPGVTSVVNSSILSGMGNGTVSSSPVANSVLNAAAGITVGVVSSQQQQQQQQQPTVNTSRFRVVKLDSTSEPFKKGRWTCTEFYEKENAVPATEGVAINKVVETVKQTPTEASSSERESTSGSSVSSSVSTLSHYTESVGSGEMMGAPAVVAPQQPPLPPAPPGLQGVALQQLEFSSPAPQSIAAVSMPQSISQSQMSQVQLQPQELSFQQKQTLQPVPLQATMSAATGIQPSPVSVVGVTAAVGQQPSVSSLAQPQLPYSQTAPPVQTPLPGAPPQQLQYGQQQPMVPAQIAPGHGQPVTQNPTSEYVQQQQQPIFQAALSSGQSSSTGTGAGISVIPVAQAQGIQLPGQPTAVQTQPAGAAGQPIGQAQTAVSTVPTGGQIASIGQQANIPTAVQQPSTQVTPSVIQQGAPPSSQVVLPAPTGIIHQGVQTRASSLPQQLVIAPQSTLVTVPPQPQGVETVAQGVVSQQLPTGSPLPSASTISVTNQVSSAAPSGMPSVPTNLVPPQNIAQPPATQNGSLVQSVSQSPLIATNINLPLAQQIPLSSTQFSTQSLAQAIGSQMEDARRPAEPSLGGLPQTMSGDSGGMSAVSDGSSSSLAAPASLFPLKVLPLTTPLVDGEDESSGASVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELIEKNSQLEQENNLLKTLASPEQLAQFQAQLQTGSPPATTQPQGTTQPPAQPASQGSGSTA

P62500-2

  • Name
    2
  • Synonyms
    TSC22D1-2
    , TSC-22
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-934: Missing
    • 935-975: GDSGGMSAVSDGSSSSLAAPASLFPLKVLPLTTPLVDGEDE → MKSQWCRPVAMDLGVYQLRHFSISFLSSLLGTENASVRLDN

P62500-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 9 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
D3YW86D3YW86_MOUSETsc22d1107
D3Z0V7D3Z0V7_MOUSETsc22d1995
H3BLI9H3BLI9_MOUSETsc22d186
H3BLH4H3BLH4_MOUSETsc22d1132
H3BK98H3BK98_MOUSETsc22d1133
H3BJV9H3BJV9_MOUSETsc22d182
H3BK07H3BK07_MOUSETsc22d1128
H3BJ00H3BJ00_MOUSETsc22d195
E9QLZ1E9QLZ1_MOUSETsc22d11077

Sequence caution

The sequence AAG41218.1 differs from that shown. Reason: Erroneous initiation
The sequence AAH58660.1 differs from that shown. Reason: Erroneous initiation
The sequence BAD32600.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for alternative sequence, compositional bias, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0353271-934in isoform 2
Compositional bias59-84Pro residues
Compositional bias85-99Polar residues
Compositional bias172-188Polar residues
Compositional bias216-240Basic residues
Alternative sequenceVSP_035328303-384in isoform 3
Sequence conflict395in Ref. 4; AAG41218
Sequence conflict396-398in Ref. 2; BAD32600
Sequence conflict449in Ref. 4; AAG41218 and 3; AAH58660
Compositional bias458-489Polar residues
Sequence conflict551in Ref. 4; AAG41218
Sequence conflict591in Ref. 4; AAG41218 and 3; AAH58660
Sequence conflict660in Ref. 2; BAD32600
Sequence conflict714in Ref. 2; BAD32600
Sequence conflict720in Ref. 4; AAG41218
Sequence conflict737in Ref. 4; AAG41218
Compositional bias930-947Polar residues
Alternative sequenceVSP_035329935-975in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X62940
EMBL· GenBank· DDBJ
CAA44712.1
EMBL· GenBank· DDBJ
mRNA
AK173322
EMBL· GenBank· DDBJ
BAD32600.1
EMBL· GenBank· DDBJ
mRNA Different initiation
BC058660
EMBL· GenBank· DDBJ
AAH58660.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AF201285
EMBL· GenBank· DDBJ
AAG41218.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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