P60952 · CDC42_CANLF

Function

function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (By similarity).
Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation (By similarity).
Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity).
Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (By similarity).

Catalytic activity

Activity regulation

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site10-17GTP (UniProtKB | ChEBI)
Binding site57-61GTP (UniProtKB | ChEBI)
Binding site115-118GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentcytoplasm
Cellular Componentdendritic spine
Cellular Componentlamellipodium membrane
Cellular Componentmembrane
Cellular Componentmembrane-bounded organelle
Cellular Componentmidbody
Cellular Componentmitotic spindle
Cellular Componentplasma membrane
Cellular Componentspindle midzone
Molecular FunctionG protein activity
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionprotein kinase binding
Biological Processactin filament organization
Biological ProcessCdc42 protein signal transduction
Biological Processcell junction assembly
Biological Processdendritic spine morphogenesis
Biological Processendocytosis
Biological Processestablishment of epithelial cell apical/basal polarity
Biological Processestablishment or maintenance of cell polarity
Biological Processphagocytosis, engulfment
Biological Processpositive regulation of cytokinesis
Biological Processpositive regulation of filopodium assembly
Biological Processpositive regulation of pinocytosis
Biological Processpositive regulation of substrate adhesion-dependent cell spreading
Biological Processregulation of attachment of spindle microtubules to kinetochore
Biological Processregulation of filopodium assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cell division control protein 42 homolog
  • EC number
  • Alternative names
    • G25K GTP-binding protein

Gene names

    • Name
      CDC42

Organism names

Accessions

  • Primary accession
    P60952
  • Secondary accessions
    • P21181
    • P25763

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor
Midbody
Cytoplasm
Cell projection, lamellipodium membrane
; Peripheral membrane protein
Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome. In its active GTP-bound form localizes to the leading edge membrane of migrating dendritic cells.

Keywords

PTM/Processing

Features

Showing features for chain, modified residue, lipidation, propeptide.

TypeIDPosition(s)Description
ChainPRO_00000304231-188Cell division control protein 42 homolog
Modified residue64Phosphotyrosine; by SRC
Modified residue188Cysteine methyl ester
Lipidation188S-geranylgeranyl cysteine
PropeptidePRO_0000030424189-191Removed in mature form

Post-translational modification

Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI.

Keywords

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with DOCK11/Zizimin2; the interaction activates CDC42 by exchanging GDP for GTP. Interacts with DOCK9; the interaction activates CDC42 by exchanging GDP for GTP. Interacts with DOCK8 (via DHR-2 domain); the interaction activates CDC42 by exchanging GDP for GTP. Interacts with IQGAP1. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1. Interacts with USP6. Interacts with NEK6. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with ITGB1BP1. Interacts with ARHGDIA; this interaction inactivates and stabilizes CDC42. Interacts with ARHGDIB; this maintains CDC42 in the inactive, GDP-bound form. Interacts in (GTP-bound form) with FNBP1L and ABI1, but only in the presence of FNBP1L. Interacts with MARCKS (By similarity).
Interacts with CD151 and ITGB1 (By similarity).

Protein-protein interaction databases

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif32-40Effector region

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

P60952-2

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    Placental
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    191
  • Mass (Da)
    21,259
  • Last updated
    2011-02-08 v2
  • Checksum
    51A437E22A4D8FFF
MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

P60952-1

  • Name
    2
  • Synonyms
    Brain
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I3MX28A0A8I3MX28_CANLFCDC42166

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_010078163in isoform 2
Alternative sequenceVSP_010079182-191in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z49944
EMBL· GenBank· DDBJ
CAA90215.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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