P60877 · SNP25_MACMU
- ProteinSynaptosomal-associated protein 25
- GeneSNAP25
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids206 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane | |
Cellular Component | neuron projection | |
Cellular Component | perinuclear region of cytoplasm | |
Cellular Component | photoreceptor inner segment | |
Cellular Component | plasma membrane | |
Cellular Component | presynapse | |
Cellular Component | SNARE complex | |
Cellular Component | synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex | |
Molecular Function | SNAP receptor activity | |
Molecular Function | syntaxin-1 binding | |
Molecular Function | voltage-gated potassium channel activity | |
Biological Process | exocytosis | |
Biological Process | synaptic vesicle fusion to presynaptic active zone membrane | |
Biological Process | synaptic vesicle priming |
Names & Taxonomy
Protein names
- Recommended nameSynaptosomal-associated protein 25
- Short namesSNAP-25
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Macaca
Accessions
- Primary accessionP60877
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity).
Colocalizes with PLCL1 at the cell membrane (By similarity).
Colocalizes with PLCL1 at the cell membrane (By similarity).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, lipidation, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000213588 | 1-206 | Synaptosomal-associated protein 25 | ||
Lipidation | 85 | S-palmitoyl cysteine | |||
Lipidation | 88 | S-palmitoyl cysteine | |||
Lipidation | 90 | S-palmitoyl cysteine | |||
Lipidation | 92 | S-palmitoyl cysteine | |||
Modified residue | 138 | Phosphothreonine | |||
Modified residue | 154 | Phosphoserine | |||
Modified residue | 187 | Phosphoserine | |||
Post-translational modification
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus (By similarity).
Recruited to the SNARE complex following binding of the SNARE complex component STX1A to STXBP1 (By similarity).
This complex binds CPLX1. Found in a complex containing SYT1, SV2B and syntaxin-1. Found in a ternary complex with STX1A and VAMP8 (By similarity).
Interacts with HSC70 and with SYT9, forming a complex with DNAJC5 (By similarity).
The interaction with SYT9 is inhibited in presence of calcium (By similarity).
Isoform 1 and isoform 2 interact with BLOC1S6. Interacts with CENPF. Interacts with EQTN. Interacts with HGS. Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. Interacts with OTOF. Interacts with RIMS1. Interacts with SNAPIN. Interacts with STXBP6. Interacts with TRIM9. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Associates with the BLOC-1 complex (By similarity).
Interacts with PLCL1 (via C2 domain) (By similarity).
Interacts with PRRT2; this interaction may impair the formation of the SNARE complex (By similarity).
Interacts with alpha-synuclein/SNCA (By similarity).
Interacts with PRPH2 (By similarity).
Interacts with ROM1 (By similarity).
Interacts with STX3 (By similarity).
Recruited to the SNARE complex following binding of the SNARE complex component STX1A to STXBP1 (By similarity).
This complex binds CPLX1. Found in a complex containing SYT1, SV2B and syntaxin-1. Found in a ternary complex with STX1A and VAMP8 (By similarity).
Interacts with HSC70 and with SYT9, forming a complex with DNAJC5 (By similarity).
The interaction with SYT9 is inhibited in presence of calcium (By similarity).
Isoform 1 and isoform 2 interact with BLOC1S6. Interacts with CENPF. Interacts with EQTN. Interacts with HGS. Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. Interacts with OTOF. Interacts with RIMS1. Interacts with SNAPIN. Interacts with STXBP6. Interacts with TRIM9. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Associates with the BLOC-1 complex (By similarity).
Interacts with PLCL1 (via C2 domain) (By similarity).
Interacts with PRRT2; this interaction may impair the formation of the SNARE complex (By similarity).
Interacts with alpha-synuclein/SNCA (By similarity).
Interacts with PRPH2 (By similarity).
Interacts with ROM1 (By similarity).
Interacts with STX3 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-23 | Disordered | |||
Region | 1-75 | Interaction with CENPF | |||
Domain | 19-81 | t-SNARE coiled-coil homology 1 | |||
Region | 111-120 | Interaction with ZDHHC17 | |||
Domain | 140-202 | t-SNARE coiled-coil homology 2 | |||
Sequence similarities
Belongs to the SNAP-25 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length206
- Mass (Da)23,315
- Last updated2004-04-13 v1
- ChecksumFBED2B082A4CB6A6
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F7GMG8 | F7GMG8_MACMU | SNAP25 | 197 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF240770 EMBL· GenBank· DDBJ | AAF64477.1 EMBL· GenBank· DDBJ | mRNA |