P60877 · SNP25_MACMU

Function

function

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentmembrane
Cellular Componentneuron projection
Cellular Componentperinuclear region of cytoplasm
Cellular Componentphotoreceptor inner segment
Cellular Componentplasma membrane
Cellular Componentpresynapse
Cellular ComponentSNARE complex
Cellular Componentsynaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex
Molecular FunctionSNAP receptor activity
Molecular Functionsyntaxin-1 binding
Molecular Functionvoltage-gated potassium channel activity
Biological Processexocytosis
Biological Processsynaptic vesicle fusion to presynaptic active zone membrane
Biological Processsynaptic vesicle priming

Names & Taxonomy

Protein names

  • Recommended name
    Synaptosomal-associated protein 25
  • Short names
    SNAP-25
  • Alternative names
    • Synaptosomal-associated 25 kDa protein

Gene names

    • Name
      SNAP25

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Macaca

Accessions

  • Primary accession
    P60877
  • Secondary accessions
    • P13795
    • P36974
    • P70557
    • P70558
    • Q8IXK3

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor
Synapse, synaptosome
Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity).
Colocalizes with PLCL1 at the cell membrane (By similarity).

Keywords

PTM/Processing

Features

Showing features for chain, lipidation, modified residue.

Type
IDPosition(s)Description
ChainPRO_00002135881-206Synaptosomal-associated protein 25
Lipidation85S-palmitoyl cysteine
Lipidation88S-palmitoyl cysteine
Lipidation90S-palmitoyl cysteine
Lipidation92S-palmitoyl cysteine
Modified residue138Phosphothreonine
Modified residue154Phosphoserine
Modified residue187Phosphoserine

Post-translational modification

Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).

Keywords

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus (By similarity).
Recruited to the SNARE complex following binding of the SNARE complex component STX1A to STXBP1 (By similarity).
This complex binds CPLX1. Found in a complex containing SYT1, SV2B and syntaxin-1. Found in a ternary complex with STX1A and VAMP8 (By similarity).
Interacts with HSC70 and with SYT9, forming a complex with DNAJC5 (By similarity).
The interaction with SYT9 is inhibited in presence of calcium (By similarity).
Isoform 1 and isoform 2 interact with BLOC1S6. Interacts with CENPF. Interacts with EQTN. Interacts with HGS. Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. Interacts with OTOF. Interacts with RIMS1. Interacts with SNAPIN. Interacts with STXBP6. Interacts with TRIM9. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Associates with the BLOC-1 complex (By similarity).
Interacts with PLCL1 (via C2 domain) (By similarity).
Interacts with PRRT2; this interaction may impair the formation of the SNARE complex (By similarity).
Interacts with alpha-synuclein/SNCA (By similarity).
Interacts with PRPH2 (By similarity).
Interacts with ROM1 (By similarity).
Interacts with STX3 (By similarity).

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-23Disordered
Region1-75Interaction with CENPF
Domain19-81t-SNARE coiled-coil homology 1
Region111-120Interaction with ZDHHC17
Domain140-202t-SNARE coiled-coil homology 2

Sequence similarities

Belongs to the SNAP-25 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    206
  • Mass (Da)
    23,315
  • Last updated
    2004-04-13 v1
  • Checksum
    FBED2B082A4CB6A6
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F7GMG8F7GMG8_MACMUSNAP25197

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF240770
EMBL· GenBank· DDBJ
AAF64477.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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