P60723 · RL4_ECOLI
- ProteinLarge ribosomal subunit protein uL4
- GenerplD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids201 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA (PubMed:3298242).
It is important during the early stages of 50S assembly (PubMed:3298242).
It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (PubMed:6170935, PubMed:7556101).
It is important during the early stages of 50S assembly (PubMed:3298242).
It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (PubMed:6170935, PubMed:7556101).
Protein uL4 is a both a transcriptional repressor and a translational repressor protein; these two functions are independent of each other. It regulates transcription of the S10 operon (to which uL4 belongs) by causing premature termination of transcription within the S10 leader; termination absolutely requires the NusA protein. uL4 controls the translation of the S10 operon by binding to its mRNA. The regions of uL4 that control regulation (residues 131-210) are different from those required for ribosome assembly (residues 89-103).
Forms part of the polypeptide exit tunnel.
Can regulate expression from Citrobacter freundii, Haemophilus influenzae, Morganella morganii, Salmonella typhimurium, Serratia marcescens, Vibrio cholerae and Yersinia enterocolitica (but not Pseudomonas aeruginosa) S10 leaders in vitro.
Miscellaneous
The erythromycin sensitive allele is dominant over the resistant allele.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | cytosolic large ribosomal subunit | |
Cellular Component | large ribosomal subunit | |
Molecular Function | DNA binding | |
Molecular Function | endoribonuclease inhibitor activity | |
Molecular Function | mRNA 5'-UTR binding | |
Molecular Function | RNA-binding transcription regulator activity | |
Molecular Function | rRNA binding | |
Molecular Function | structural constituent of ribosome | |
Molecular Function | translation repressor activity | |
Biological Process | cytoplasmic translation | |
Biological Process | DNA-templated transcription termination | |
Biological Process | negative regulation of cytoplasmic translation | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | negative regulation of translation | |
Biological Process | response to antibiotic | |
Biological Process | ribosome assembly | |
Biological Process | transcriptional attenuation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameLarge ribosomal subunit protein uL4
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP60723
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis, natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 40-88 | Regulates the S10 operon normally. Assembles into ribosomes in vivo. | ||||
Sequence: Missing | ||||||
Natural variant | 63 | in strain: N282; confers erythromycin resistance. Ribosomes bind erythromycin poorly and have reduced peptidyltransferase activity. 50S subunits assemble normally, even in the presence of drug, but assembly is cold-sensitive at 20 degrees Celsius | ||||
Sequence: K → E | ||||||
Mutagenesis | 67-103 | Regulates the S10 operon normally. Does not assemble into ribosomes in vivo. | ||||
Sequence: Missing | ||||||
Mutagenesis | 93-101 | Regulates the S10 operon normally. Not stably associated with the ribosome in vivo. | ||||
Sequence: Missing | ||||||
Mutagenesis | 131 | Does not regulate the S10 operon; assembles into ribosomes in vivo. | ||||
Sequence: T → I | ||||||
Mutagenesis | 134 | Does not regulate the S10 operon in vivo. | ||||
Sequence: L → P | ||||||
Mutagenesis | 160 | Does not regulate the S10 operon; assembles into ribosomes in vivo. | ||||
Sequence: A → V | ||||||
Mutagenesis | 167 | Does not regulate the S10 operon in vivo. | ||||
Sequence: V → D | ||||||
Mutagenesis | 171-201 | Loss of S10 operon regulation. Assembles into ribosomes in vivo. | ||||
Sequence: DATGIDPVSLIAFDKVVMTADAVKQVEEMLA → RRSK |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000129215 | 1-201 | Large ribosomal subunit protein uL4 | |||
Sequence: MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA |
Proteomic databases
Interaction
Subunit
Part of the 50S ribosomal subunit (PubMed:10094780, PubMed:11511371, PubMed:12809609, PubMed:16272117, PubMed:24844575, PubMed:25310980, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:3298242, PubMed:34403461, PubMed:34504068, PubMed:7556101, Ref.1). In TnaC-stalled ribosomes forms part of the binding pocket for L-Trp with the leader peptide and uL22 (PubMed:34403461, PubMed:34504068).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P60723 | coaD P0A6I6 | 2 | EBI-545597, EBI-553173 | |
BINARY | P60723 | def P0A6K3 | 4 | EBI-545597, EBI-548913 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 44-71 | Disordered | ||||
Sequence: RAQKTRAEVTGSGKKPWRQKGTGRARSG |
Sequence similarities
Belongs to the universal ribosomal protein uL4 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length201
- Mass (Da)22,087
- Last updated1986-07-21 v1
- Checksum3A953206B0F083B5
Mass Spectrometry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X02613 EMBL· GenBank· DDBJ | CAA26461.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U18997 EMBL· GenBank· DDBJ | AAA58116.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC76344.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE77972.1 EMBL· GenBank· DDBJ | Genomic DNA |