P59936 · KA121_TITSE

Function

function

Potently blocks Kv1.3/KCNA3, Kv1.2/KCNA2, and Shaker potassium channels (PubMed:24590385) and inhibits high conductance calcium-activated potassium channels (PubMed:10082164).
Is potent against Kv1.3/KCNN3 (IC50=0.55 nM) and Kv1.2/KCNN2 (IC50=6.19 nM) (PubMed:24590385).
It also stimulates the release of NO, IL-6 and TNF-alpha in J774.1 cells and presents a pro-inflammatory activity in mice (PubMed:21549737, PubMed:23085190).

Miscellaneous

The N-terminal C2-C5 disulfide bridge unique to this toxin does not appear to confer stability to the protein.
Negative results: inhibits with a low efficiency Kv1.1/KCNA1, Kv1.5/KCNA5, Kv1.6/KCNA6, Kv4.3/KCND3, Kv7.1/KCNQ1, Kv7.2/KCNQ2, Kv7.4/KCNQ4, and ERG/KCNH2, and does not inhibit Kv1.4/KCNA4, Kv2.1/KCNB1, and Kv3.1/KCNC1.

Features

Showing features for site.

16251015202530354045505560
MQVFYGLLLMFILCSTIHLSEQWCSTCLDLACGASRECYDPCFKAFGRAHGKCMNNKCRCYT
TypeIDPosition(s)Description
Site52Basic residue of the functional dyad
Site61Aromatic residue of the functional dyad

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functionion channel inhibitor activity
Molecular Functionpotassium channel regulator activity
Molecular Functiontoxin activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Potassium channel toxin alpha-KTx 12.1
  • Alternative names
    • Butantoxin
      (BuTX
      )
    • Tityustoxin-6
      (Ts6
      )
    • TsTX-IV

Organism names

Accessions

  • Primary accession
    P59936
  • Secondary accessions
    • A0A218QXB7

Subcellular Location

Keywords

Phenotypes & Variants

Toxic dose

LD50 is 826 ug/kg by intravenous injection into mice.

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_000006683823-62Potassium channel toxin alpha-KTx 12.1
Disulfide bond24↔27
Disulfide bond32↔53
Disulfide bond38↔58
Disulfide bond42↔60

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Family & Domains

Domain

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    62
  • Mass (Da)
    7,083
  • Last updated
    2022-08-03 v3
  • Checksum
    73A73207EE885334
MQVFYGLLLMFILCSTIHLSEQWCSTCLDLACGASRECYDPCFKAFGRAHGKCMNNKCRCYT

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict62in Ref. 4; AA sequence

Mass Spectrometry

Molecular mass is 4,508.08 Da. Determined by MALDI.
Molecular mass is 4,505.8 Da. Determined by Electrospray.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GEUW01000052
EMBL· GenBank· DDBJ
JAW06993.1
EMBL· GenBank· DDBJ
mRNA
GEUW01000010
EMBL· GenBank· DDBJ
JAW07035.1
EMBL· GenBank· DDBJ
mRNA
MK159306
EMBL· GenBank· DDBJ
QCZ41150.1
EMBL· GenBank· DDBJ
mRNA
MT450709
EMBL· GenBank· DDBJ
QPD99045.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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